##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BclI
BglI BlnI BlpI Bpu1102I BsaBI
BsgI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII BssSI BstAPI BstBI
CelII ClaI CpoI DraIII Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
FspI HaeII KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmeI PshBI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 4459 GACGTC GACGT^C
AccI 3581 GTATAC GT^[AC][GT]AC
AccII 1148 CGCG CG^CG
AccIII 1184 TCCGGA T^CCGGA
AclI 1506 AACGTT AA^CGTT
AcyI 4459 GACGTC G[GA]^CG[CT]C
AgeI 1337 ACCGGT A^CCGGT
ApaI 2569 GGGCCC GGGCC^C
Asp700I 3794 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 4186 TGGCCA TGG^CCA
BamHI 2631 GGATCC G^GATCC
BbrPI 1609 CACGTG CAC^GTG
BcgI 2895 GCAGGGCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1791 GTATCC GTATCC(6/5)
BglII 3508 AGATCT A^GATCT
BsaAI 1609 CACGTG [CT]AC^GT[GA]
BsaHI 4459 GACGTC G[GA]^CG[CT]C
BsaI 2598 GGTCTC GGTCTC(1/5)
BseAI 1184 TCCGGA T^CCGGA
BsiEI 3491 CGGCCG CG[GA][CT]^CG
BspEI 1184 TCCGGA T^CCGGA
BspLU11I 2727 ACATGT A^CATGT
BsrBI 310 GAGCGG CCGCTC(-3/-3)
BsrDI 2825 GCAATG GCAATG(2/0)
BsrFI 1337 ACCGGT [GA]^CCGG[CT]
Bst1107I 3581 GTATAC GTA^TAC
BstEII 4020 GGTCACC G^GT[ACGT]ACC
BstPI 4020 GGTCACC G^GT[ACGT]ACC
BstUI 1148 CGCG CG^CG
BstZ17I 3581 GTATAC GTA^TAC
Cfr10I 1337 ACCGGT [GA]^CCGG[CT]
DraI 3830 TTTAAA TTT^AAA
DrdI 832 GACAACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3491 CGGCCG C^GGCCG
EclXI 3491 CGGCCG C^GGCCG
Eco52I 3491 CGGCCG C^GGCCG
EcoO65I 4020 GGTCACC G^GT[ACGT]ACC
Hin1I 4459 GACGTC G[GA]^CG[CT]C
HincII 3851 GTTAAC GT[CT]^[GA]AC
HindII 3851 GTTAAC GT[CT]^[GA]AC
HpaI 3851 GTTAAC GTT^AAC
MluNI 4186 TGGCCA TGG^CCA
MroI 1184 TCCGGA T^CCGGA
MscI 4186 TGGCCA TGG^CCA
MvnI 1148 CGCG CG^CG
PinAI 1337 ACCGGT A^CCGGT
PmaCI 1609 CACGTG CAC^GTG
PmlI 1609 CACGTG CAC^GTG
Psp1406I 1506 AACGTT AA^CGTT
ScaI 3903 AGTACT AGT^ACT
SmaI 287 CCCGGG CCC^GGG
SspI 4165 AATATT AAT^ATT
StuI 353 AGGCCT AGG^CCT
XmaCI 287 CCCGGG C^CCGGG
XmaI 287 CCCGGG C^CCGGG
XmnI 3794 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1764 GAATTC [GA]^AATT[CT]
4180 GAATTC
AflII 871 CTTAAG C^TTAAG
913 CTTAAG
AflIII 1608 ACACGT A^C[GA][CT]GT
2727 ACATGT
AlwNI 2877 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3740 CAGCATCTG
AocI 2418 CCTGAGG CC^T[ACGT]AGG
4015 CCTAAGG
ApoI 1764 GAATTC [GA]^AATT[CT]
4180 GAATTC
AvaI 287 CCCGGG C^[CT]CG[GA]G
2066 CCCGAG
BbvCI 1162 CCTCAGC CCTCAGC(-5/-2)
4364 GCTGAGG
BfrI 871 CTTAAG C^TTAAG
913 CTTAAG
BsaWI 1184 TCCGGA [AT]^CCGG[AT]
1337 ACCGGT
BsmI 669 GAATGC GAATGC(1/-1)
2742 GAATGC
BsoBI 287 CCCGGG C^[CT]CG[GA]G
2066 CCCGAG
Bsu36I 2418 CCTGAGG CC^T[ACGT]AGG
4015 CCTAAGG
CfoI 183 GCGC GCG^C
1147 GCGC
EaeI 3491 CGGCCG [CT]^GGCC[GA]
4186 TGGCCA
Eco81I 2418 CCTGAGG CC^T[ACGT]AGG
4015 CCTAAGG
EcoRI 1764 GAATTC G^AATTC
4180 GAATTC
HgaI 876 GACGC GACGC(5/10)
517 GCGTC
HhaI 183 GCGC GCG^C
1147 GCGC
HinP1I 183 GCGC G^CGC
1147 GCGC
HindIII 1124 AAGCTT A^AGCTT
1277 AAGCTT
NcoI 1524 CCATGG C^CATGG
2159 CCATGG
PpuMI 2878 AGGTCCT [GA]G^G[AT]CC[CT]
2953 AGGACCC
PshAI 1888 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3620 GACAGCTGTC
SapI 907 GAAGAGC GCTCTTC(1/4)
4041 GAAGAGC
XbaI 2047 TCTAGA T^CTAGA
2233 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII