##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbeI BclI
BglII BlnI BsaBI BsaHI BsiEI
BspDI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KspI MamI MfeI MluI MunI
NarI NdeI NheI NotI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AflII 4020 CTTAAG C^TTAAG
Aor51HI 1517 AGCGCT AGC^GCT
BamHI 1557 GGATCC G^GATCC
BbrPI 2265 CACGTG CAC^GTG
BbsI 2650 GAAGAC GAAGAC(2/6)
BciVI 1622 GTATCC GTATCC(6/5)
BfrI 4020 CTTAAG C^TTAAG
BpuAI 2650 GAAGAC GAAGAC(2/6)
Bsp1407I 2027 TGTACA T^GTACA
BsrBI 1784 GAGCGG CCGCTC(-3/-3)
BsrDI 1006 GCAATG GCAATG(2/0)
BsrGI 2027 TGTACA T^GTACA
BstEII 4323 GGTGACC G^GT[ACGT]ACC
BstPI 4323 GGTGACC G^GT[ACGT]ACC
BstYI 1557 GGATCC [GA]^GATC[CT]
DrdI 1267 GACAATAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1517 AGCGCT AGC^GCT
EcoO65I 4323 GGTGACC G^GT[ACGT]ACC
HgaI 4620 GCGTC GACGC(5/10)
HincII 1866 GTTGAC GT[CT]^[GA]AC
HindII 1866 GTTGAC GT[CT]^[GA]AC
HindIII 1376 AAGCTT A^AGCTT
MflI 1557 GGATCC [GA]^GATC[CT]
NruI 732 TCGCGA TCG^CGA
PaeR7I 1205 CTCGAG C^TCGAG
PmaCI 2265 CACGTG CAC^GTG
PmlI 2265 CACGTG CAC^GTG
SphI 1079 GCATGC GCATG^C
SspBI 2027 TGTACA T^GTACA
SspI 2599 AATATT AAT^ATT
XbaI 4341 TCTAGA T^CTAGA
XhoI 1205 CTCGAG C^TCGAG
XhoII 1557 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1605 GGTACC G^GTACC
4146 GGTACC
AccI 373 GTCTAC GT^[AC][GT]AC
3648 GTAGAC
AccII 733 CGCG CG^CG
3095 CGCG
AccIII 2445 TCCGGA T^CCGGA
2523 TCCGGA
Alw44I 579 GTGCAC G^TGCAC
2667 GTGCAC
ApaLI 579 GTGCAC G^TGCAC
2667 GTGCAC
Asp718I 1605 GGTACC G^GTACC
4146 GGTACC
AspI 3401 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4783 GACTCTGTC
BsaAI 1528 CACGTA [CT]AC^GT[GA]
2265 CACGTG
BsaI 4591 GAGACC GGTCTC(1/5)
203 GGTCTC
BseAI 2445 TCCGGA T^CCGGA
2523 TCCGGA
BsiWI 1055 CGTACG C^GTACG
1530 CGTACG
BsmI 2010 GAATGC GAATGC(1/-1)
3310 GAATGC
BspEI 2445 TCCGGA T^CCGGA
2523 TCCGGA
BsrFI 1408 GCCGGC [GA]^CCGG[CT]
4508 GCCGGC
BstUI 733 CGCG CG^CG
3095 CGCG
Cfr10I 1408 GCCGGC [GA]^CCGG[CT]
4508 GCCGGC
KpnI 1605 GGTACC GGTAC^C
4146 GGTACC
MroI 2445 TCCGGA T^CCGGA
2523 TCCGGA
MvnI 733 CGCG CG^CG
3095 CGCG
NaeI 1408 GCCGGC GCC^GGC
4508 GCCGGC
NcoI 1672 CCATGG C^CATGG
3475 CCATGG
NgoMIV 1408 GCCGGC G^CCGGC
4508 GCCGGC
NspI 1079 GCATGC [GA]CATG^[CT]
2539 ACATGC
PflFI 3401 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4783 GACTCTGTC
SacI 169 GAGCTC GAGCT^C
3371 GAGCTC
SapI 1495 GAAGAGC GCTCTTC(1/4)
3373 GCTCTTC
SmaI 1307 CCCGGG CCC^GGG
4715 CCCGGG
TaqI 1206 TCGA T^CGA
4589 TCGA
Tth111I 3401 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4783 GACTCTGTC
TthHB8I 1206 TCGA T^CGA
4589 TCGA
XmaCI 1307 CCCGGG C^CCGGG
4715 CCCGGG
XmaI 1307 CCCGGG C^CCGGG
4715 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI