##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BcgI
BclI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NspV PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 1048 CGCG CG^CG
AcyI 2746 GGCGTC G[GA]^CG[CT]C
Alw44I 1586 GTGCAC G^TGCAC
Aor51HI 3994 AGCGCT AGC^GCT
ApaLI 1586 GTGCAC G^TGCAC
BamHI 3188 GGATCC G^GATCC
BanI 2778 GGCACC G^G[CT][GA]CC
BbsI 1517 GAAGAC GAAGAC(2/6)
BciVI 2385 GGATAC GTATCC(6/5)
BpuAI 1517 GAAGAC GAAGAC(2/6)
BsaBI 3834 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2746 GGCGTC G[GA]^CG[CT]C
BsaWI 3721 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3932 TGTACA T^GTACA
BspMI 397 GCAGGT ACCTGC(4/8)
BsrGI 3932 TGTACA T^GTACA
BstEII 2472 GGTGACC G^GT[ACGT]ACC
BstPI 2472 GGTGACC G^GT[ACGT]ACC
BstUI 1048 CGCG CG^CG
Eco47III 3994 AGCGCT AGC^GCT
EcoO65I 2472 GGTGACC G^GT[ACGT]ACC
EcoT22I 1359 ATGCAT ATGCA^T
FauI 521 CCCGC CCCGC(4/6)
Hin1I 2746 GGCGTC G[GA]^CG[CT]C
MamI 3834 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1568 CAATTG C^AATTG
MspA1I 702 CAGCTG C[AC]G^C[GT]G
MunI 1568 CAATTG C^AATTG
MvnI 1048 CGCG CG^CG
NdeI 2160 CATATG CA^TATG
NsiI 1359 ATGCAT ATGCA^T
PvuII 702 CAGCTG CAG^CTG
SacI 878 GAGCTC GAGCT^C
SapI 530 GAAGAGC GCTCTTC(1/4)
SspBI 3932 TGTACA T^GTACA
StuI 891 AGGCCT AGG^CCT
XbaI 4040 TCTAGA T^CTAGA
XcmI 3594 CCAAGGGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 1676 CTTAAG C^TTAAG
3575 CTTAAG
AocI 320 CCTGAGG CC^T[ACGT]AGG
972 CCTTAGG
ApaI 863 GGGCCC GGGCC^C
4028 GGGCCC
AspI 586 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4217 GACCCTGTC
BalI 339 TGGCCA TGG^CCA
3606 TGGCCA
BbvCI 1482 GCTGAGG CCTCAGC(-5/-2)
4007 GCTGAGG
BfrI 1676 CTTAAG C^TTAAG
3575 CTTAAG
BglI 856 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3637 GCCAAAGAGGC
BglII 190 AGATCT A^GATCT
2943 AGATCT
BlpI 1792 GCTAAGC GC^T[ACGT]AGC
1995 GCTTAGC
Bpu1102I 1792 GCTAAGC GC^T[ACGT]AGC
1995 GCTTAGC
BspHI 1102 TCATGA T^CATGA
1724 TCATGA
Bst1107I 1551 GTATAC GTA^TAC
1627 GTATAC
BstZ17I 1551 GTATAC GTA^TAC
1627 GTATAC
Bsu36I 320 CCTGAGG CC^T[ACGT]AGG
972 CCTTAGG
CelII 1792 GCTAAGC GC^T[ACGT]AGC
1995 GCTTAGC
DraI 2172 TTTAAA TTT^AAA
3166 TTTAAA
Eco81I 320 CCTGAGG CC^T[ACGT]AGG
972 CCTTAGG
HaeII 568 GGCGCT [GA]GCGC^[CT]
3994 AGCGCT
MluNI 339 TGGCCA TGG^CCA
3606 TGGCCA
MscI 339 TGGCCA TGG^CCA
3606 TGGCCA
PacI 1871 TTAATTAA TTAAT^TAA
1894 TTAATTAA
PflFI 586 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4217 GACCCTGTC
PflMI 830 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2374 CCAAGCACTGG
RcaI 1102 TCATGA T^CATGA
1724 TCATGA
SmaI 466 CCCGGG CCC^GGG
4031 CCCGGG
Tth111I 586 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4217 GACCCTGTC
Van91I 830 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2374 CCAAGCACTGG
XmaCI 466 CCCGGG C^CCGGG
4031 CCCGGG
XmaI 466 CCCGGG C^CCGGG
4031 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI