##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII BamHI BbeI BbrPI
BclI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MroI MspA1I MunI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 504 CGCG CG^CG
AhdI 2258 GACGGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2112 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 499 GGGCCC GGGCC^C
AspEI 2258 GACGGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 3901 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 459 GGATAC GTATCC(6/5)
BglI 3996 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1869 AGATCT A^GATCT
BsgI 26 GTGCAG GTGCAG(16/14)
BsmI 2494 GCATTC GAATGC(1/-1)
Bsp1407I 2988 TGTACA T^GTACA
BspDI 2596 ATCGAT AT^CGAT
BspMI 4427 ACCTGC ACCTGC(4/8)
BsrFI 453 ACCGGC [GA]^CCGG[CT]
BsrGI 2988 TGTACA T^GTACA
BstEII 254 GGTGACC G^GT[ACGT]ACC
BstPI 254 GGTGACC G^GT[ACGT]ACC
BstUI 504 CGCG CG^CG
Cfr10I 453 ACCGGC [GA]^CCGG[CT]
ClaI 2596 ATCGAT AT^CGAT
DsaI 3369 CCATGG C^C[GA][CT]GG
Eam1105I 2258 GACGGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 254 GGTGACC G^GT[ACGT]ACC
EcoRI 488 GAATTC G^AATTC
HpaI 1102 GTTAAC GTT^AAC
MvnI 504 CGCG CG^CG
NcoI 3369 CCATGG C^CATGG
NdeI 4115 CATATG CA^TATG
NheI 907 GCTAGC G^CTAGC
PflMI 573 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 680 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 691 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 479 GTCGAC G^TCGAC
SmaI 686 CCCGGG CCC^GGG
SspBI 2988 TGTACA T^GTACA
SspI 2215 AATATT AAT^ATT
StuI 2648 AGGCCT AGG^CCT
Van91I 573 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 686 CCCGGG C^CCGGG
XmaI 686 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 539 CTTAAG C^TTAAG
1303 CTTAAG
AspI 473 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3818 GACGGAGTC
AvaI 686 CCCGGG C^[CT]CG[GA]G
2226 CTCGGG
AvrII 1755 CCTAGG C^CTAGG
1879 CCTAGG
BanI 990 GGTGCC G^G[CT][GA]CC
3941 GGTGCC
BanII 299 GGGCTC G[GA]GC[CT]^C
499 GGGCCC
BbsI 158 GTCTTC GAAGAC(2/6)
4124 GTCTTC
BbvCI 633 CCTCAGC CCTCAGC(-5/-2)
3912 CCTCAGC
BcnI 686 CCCGG CC^[GC]GG
687 CCGGG
BfrI 539 CTTAAG C^TTAAG
1303 CTTAAG
BlnI 1755 CCTAGG C^CTAGG
1879 CCTAGG
BpuAI 158 GTCTTC GAAGAC(2/6)
4124 GTCTTC
BsaBI 58 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
843 GATTTTCATC
BsmBI 3816 GAGACG CGTCTC(1/5)
3983 GAGACG
BsoBI 686 CCCGGG C^[CT]CG[GA]G
2226 CTCGGG
BsrDI 1161 GCAATG GCAATG(2/0)
3719 GCAATG
DraII 498 GGGGCCC [GA]G^G[ACGT]CC[CT]
680 GGGACCC
EarI 125 CTCTTC CTCTTC(1/4)
2071 GAAGAG
EcoO109I 498 GGGGCCC [GA]G^G[ACGT]CC[CT]
680 GGGACCC
HaeII 144 AGCGCC [GA]GCGC^[CT]
357 GGCGCT
HincII 479 GTCGAC GT[CT]^[GA]AC
1102 GTTAAC
HindII 479 GTCGAC GT[CT]^[GA]AC
1102 GTTAAC
Ksp632I 125 CTCTTC CTCTTC(1/4)
2071 GAAGAG
MamI 58 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
843 GATTTTCATC
NciI 686 CCCGG CC^[GC]GG
687 CCGGG
PflFI 473 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3818 GACGGAGTC
ScaI 591 AGTACT AGT^ACT
2993 AGTACT
SfcI 1266 CTGTAG C^T[GA][CT]AG
2281 CTATAG
SpeI 1567 ACTAGT A^CTAGT
3249 ACTAGT
TaqI 480 TCGA T^CGA
2597 TCGA
Tsp45I 255 GTGAC ^GT[GC]AC
2531 GTCAC
Tth111I 473 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3818 GACGGAGTC
TthHB8I 480 TCGA T^CGA
2597 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaII BalI
BbvI BfaI BmyI BpmI BsaAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspLU11I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII