##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AgeI Alw44I ApaLI
AscI Asp718I AspI AviII BbrPI
BcgI BcnI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoO65I EcoRI FseI FspI
KpnI KspI MfeI MluI MunI
NciI NcoI NdeI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PvuI RcaI
RsrII SacI SacII SapI SexAI
SfiI SmaI SphI SspBI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 219 TCCGGA T^CCGGA
AflII 1299 CTTAAG C^TTAAG
AflIII 2697 ACATGT A^C[GA][CT]GT
AhdI 4178 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 393 AGCGCT AGC^GCT
ApaI 3163 GGGCCC GGGCC^C
Asp700I 958 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4178 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3442 CCTAGG C^CTAGG
BamHI 867 GGATCC G^GATCC
BbvCI 2625 CCTCAGC CCTCAGC(-5/-2)
BciVI 1403 GGATAC GTATCC(6/5)
BclI 3001 TGATCA T^GATCA
BfrI 1299 CTTAAG C^TTAAG
BglII 999 AGATCT A^GATCT
BlnI 3442 CCTAGG C^CTAGG
BsaAI 1406 TACGTA [CT]AC^GT[GA]
BsaWI 219 TCCGGA [AT]^CCGG[AT]
BseAI 219 TCCGGA T^CCGGA
BspEI 219 TCCGGA T^CCGGA
BspLU11I 2697 ACATGT A^CATGT
BsrBI 77 GAGCGG CCGCTC(-3/-3)
BsrDI 739 CATTGC GCAATG(2/0)
BstBI 606 TTCGAA TT^CGAA
BstXI 3933 CCATAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 3226 TTTAAA TTT^AAA
Eam1105I 4178 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3313 CTCTTC CTCTTC(1/4)
Eco47III 393 AGCGCT AGC^GCT
EcoRV 365 GATATC GAT^ATC
EcoT22I 3667 ATGCAT ATGCA^T
FbaI 3001 TGATCA T^GATCA
HpaI 1826 GTTAAC GTT^AAC
Ksp632I 3313 CTCTTC CTCTTC(1/4)
MroI 219 TCCGGA T^CCGGA
MslI 4059 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 2190 CAGCTG C[AC]G^C[GT]G
NaeI 124 GCCGGC GCC^GGC
NgoMIV 124 GCCGGC G^CCGGC
NsiI 3667 ATGCAT ATGCA^T
NspV 606 TTCGAA TT^CGAA
PpuMI 635 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2107 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2190 CAGCTG CAG^CTG
SbfI 4 CCTGCAGG CCTGCA^GG
ScaI 1234 AGTACT AGT^ACT
SfuI 606 TTCGAA TT^CGAA
SgrAI 123 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 1406 TACGTA TAC^GTA
SpeI 1822 ACTAGT A^CTAGT
Sse8387I 4 CCTGCAGG CCTGCA^GG
XbaI 1670 TCTAGA T^CTAGA
XcmI 1873 CCACTTTATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 958 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 150 GTCGAC GT^[AC][GT]AC
527 GTCGAC
AccII 74 CGCG CG^CG
236 CGCG
AclI 163 AACGTT AA^CGTT
2266 AACGTT
AcsI 2412 AAATTC [GA]^AATT[CT]
3981 AAATTC
AlwNI 185 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1104 CAGCTTCTG
ApoI 2412 AAATTC [GA]^AATT[CT]
3981 AAATTC
AspHI 482 GTGCTC G[AT]GC[AT]^C
548 GAGCAC
AvaI 1584 CCCGAG C^[CT]CG[GA]G
4079 CTCGGG
BalI 2794 TGGCCA TGG^CCA
3997 TGGCCA
BbeI 121 GGCGCC GGCGC^C
2654 GGCGCC
BglI 653 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1950 GCCCTTGTGGC
BlpI 230 GCTCAGC GC^T[ACGT]AGC
1680 GCTTAGC
Bpu1102I 230 GCTCAGC GC^T[ACGT]AGC
1680 GCTTAGC
BseRI 461 CTCCTC GAGGAG(10/8)
1418 GAGGAG
BsgI 4150 CTGCAC GTGCAG(16/14)
2563 GTGCAG
BsiHKAI 482 GTGCTC G[AT]GC[AT]^C
548 GAGCAC
BsmI 642 GAATGC GAATGC(1/-1)
3669 GCATTC
BsoBI 1584 CCCGAG C^[CT]CG[GA]G
4079 CTCGGG
BsrFI 124 GCCGGC [GA]^CCGG[CT]
932 GCCGGT
BstAPI 818 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4119 GCAGAGGTTGC
BstUI 74 CGCG CG^CG
236 CGCG
CelII 230 GCTCAGC GC^T[ACGT]AGC
1680 GCTTAGC
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
932 GCCGGT
DraIII 476 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
693 CACACTGTG
DsaI 948 CCGTGG C^C[GA][CT]GG
3710 CCGTGG
EcoNI 1245 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1992 CCTGGCTAAGG
HgaI 227 GACGC GACGC(5/10)
1053 GACGC
KasI 121 GGCGCC G^GCGCC
2654 GGCGCC
MluNI 2794 TGGCCA TGG^CCA
3997 TGGCCA
MscI 2794 TGGCCA TGG^CCA
3997 TGGCCA
MvnI 74 CGCG CG^CG
236 CGCG
NarI 121 GGCGCC GG^CGCC
2654 GGCGCC
Psp1406I 163 AACGTT AA^CGTT
2266 AACGTT
SalI 150 GTCGAC G^TCGAC
527 GTCGAC
SfoI 121 GGCGCC GGC^GCC
2654 GGCGCC
Tsp45I 1432 GTCAC ^GT[GC]AC
4166 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AocI AseI AsnI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I XhoII