##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BbeI BciVI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HindIII HpaI KasI KpnI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3756 GACGTC GACGT^C
AcsI 2961 GAATTC [GA]^AATT[CT]
AflII 1756 CTTAAG C^TTAAG
Alw44I 2086 GTGCAC G^TGCAC
ApaLI 2086 GTGCAC G^TGCAC
ApoI 2961 GAATTC [GA]^AATT[CT]
BbvCI 1528 GCTGAGG CCTCAGC(-5/-2)
BcgI 3448 CGACCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1756 CTTAAG C^TTAAG
BglII 4055 AGATCT A^GATCT
BsaBI 2571 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1167 GAGACC GGTCTC(1/5)
BsmI 2256 GAATGC GAATGC(1/-1)
BspHI 1666 TCATGA T^CATGA
BstEII 820 GGTGACC G^GT[ACGT]ACC
BstPI 820 GGTGACC G^GT[ACGT]ACC
DraIII 2392 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1185 GACACTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 820 GGTGACC G^GT[ACGT]ACC
EcoRI 2961 GAATTC G^AATTC
EcoT22I 3949 ATGCAT ATGCA^T
HaeII 2346 AGCGCC [GA]GCGC^[CT]
HincII 78 GTTGAC GT[CT]^[GA]AC
HindII 78 GTTGAC GT[CT]^[GA]AC
KspI 2007 CCGCGG CCGC^GG
MamI 2571 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2215 CATATG CA^TATG
NheI 1128 GCTAGC G^CTAGC
NsiI 3949 ATGCAT ATGCA^T
PpuMI 1904 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1666 TCATGA T^CATGA
SacI 1337 GAGCTC GAGCT^C
SacII 2007 CCGCGG CCGC^GG
SbfI 1040 CCTGCAGG CCTGCA^GG
SphI 974 GCATGC GCATG^C
Sse8387I 1040 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 2558 GGCGTC G[GA]^CG[CT]C
3756 GACGTC
AocI 15 CCTGAGG CC^T[ACGT]AGG
2476 CCTGAGG
AvrII 2797 CCTAGG C^CTAGG
3475 CCTAGG
BbrPI 1605 CACGTG CAC^GTG
2880 CACGTG
BclI 1365 TGATCA T^GATCA
1919 TGATCA
BlnI 2797 CCTAGG C^CTAGG
3475 CCTAGG
BlpI 3396 GCTGAGC GC^T[ACGT]AGC
4192 GCTCAGC
Bpu1102I 3396 GCTGAGC GC^T[ACGT]AGC
4192 GCTCAGC
BsaAI 1605 CACGTG [CT]AC^GT[GA]
2880 CACGTG
BsaHI 2558 GGCGTC G[GA]^CG[CT]C
3756 GACGTC
BsmBI 3758 CGTCTC CGTCTC(1/5)
3754 GAGACG
Bsp1407I 3383 TGTACA T^GTACA
3589 TGTACA
BsrDI 4279 CATTGC GCAATG(2/0)
3293 GCAATG
BsrFI 1229 GCCGGC [GA]^CCGG[CT]
3369 GCCGGC
BsrGI 3383 TGTACA T^GTACA
3589 TGTACA
BssSI 526 CACGAG CACGAG(-5/-1)
3025 CTCGTG
BstAPI 2879 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3076 GCAGGCCCTGC
Bsu36I 15 CCTGAGG CC^T[ACGT]AGG
2476 CCTGAGG
CelII 3396 GCTGAGC GC^T[ACGT]AGC
4192 GCTCAGC
Cfr10I 1229 GCCGGC [GA]^CCGG[CT]
3369 GCCGGC
Eco81I 15 CCTGAGG CC^T[ACGT]AGG
2476 CCTGAGG
FbaI 1365 TGATCA T^GATCA
1919 TGATCA
Hin1I 2558 GGCGTC G[GA]^CG[CT]C
3756 GACGTC
NaeI 1229 GCCGGC GCC^GGC
3369 GCCGGC
NgoMIV 1229 GCCGGC G^CCGGC
3369 GCCGGC
PmaCI 1605 CACGTG CAC^GTG
2880 CACGTG
PmlI 1605 CACGTG CAC^GTG
2880 CACGTG
SexAI 3180 ACCTGGT A^CC[AT]GGT
4200 ACCTGGT
SspBI 3383 TGTACA T^GTACA
3589 TGTACA
TaqI 44 TCGA T^CGA
2959 TCGA
TthHB8I 44 TCGA T^CGA
2959 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI