##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AclI AflII AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BbrPI BclI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BspDI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HindIII
HpaI MamI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3702 GACGTC GACGT^C
Acc65I 2644 GGTACC G^GTACC
Alw44I 4313 GTGCAC G^TGCAC
ApaLI 4313 GTGCAC G^TGCAC
Asp718I 2644 GGTACC G^GTACC
AspI 856 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4002 GGATCC G^GATCC
BbeI 2620 GGCGCC GGCGC^C
BcgI 688 CGACGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 488 GGATAC GTATCC(6/5)
BlpI 1508 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1508 GCTGAGC GC^T[ACGT]AGC
BsmI 281 GCATTC GAATGC(1/-1)
BspLU11I 2548 ACATGT A^CATGT
BstAPI 1361 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1508 GCTGAGC GC^T[ACGT]AGC
EcoRI 1964 GAATTC G^AATTC
HincII 298 GTTGAC GT[CT]^[GA]AC
HindII 298 GTTGAC GT[CT]^[GA]AC
KasI 2620 GGCGCC G^GCGCC
KpnI 2644 GGTACC GGTAC^C
MfeI 4338 CAATTG C^AATTG
MluI 3782 ACGCGT A^CGCGT
MunI 4338 CAATTG C^AATTG
NarI 2620 GGCGCC GG^CGCC
PaeR7I 1092 CTCGAG C^TCGAG
PflFI 856 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1845 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1194 GAGCTC GAGCT^C
SapI 1469 GAAGAGC GCTCTTC(1/4)
SfoI 2620 GGCGCC GGC^GCC
SmaI 3530 CCCGGG CCC^GGG
SmlI 1092 CTCGAG C^T[CT][GA]AG
SphI 1078 GCATGC GCATG^C
Tth111I 856 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1092 CTCGAG C^TCGAG
XmaCI 3530 CCCGGG C^CCGGG
XmaI 3530 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 189 TCCGGA T^CCGGA
819 TCCGGA
AcsI 1964 GAATTC [GA]^AATT[CT]
3338 AAATTC
AhdI 1703 GACGGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4320 GACTGTGAGTC
ApoI 1964 GAATTC [GA]^AATT[CT]
3338 AAATTC
Asp700I 121 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3324 GAAAGTGTTC
AspEI 1703 GACGGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4320 GACTGTGAGTC
BbvCI 948 CCTCAGC CCTCAGC(-5/-2)
2806 GCTGAGG
BseAI 189 TCCGGA T^CCGGA
819 TCCGGA
Bsp1407I 258 TGTACA T^GTACA
708 TGTACA
BspEI 189 TCCGGA T^CCGGA
819 TCCGGA
BspMI 1039 ACCTGC ACCTGC(4/8)
400 GCAGGT
BsrDI 2156 CATTGC GCAATG(2/0)
693 GCAATG
BsrGI 258 TGTACA T^GTACA
708 TGTACA
Eam1105I 1703 GACGGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4320 GACTGTGAGTC
EcoT22I 1577 ATGCAT ATGCA^T
1982 ATGCAT
HaeII 2620 GGCGCC [GA]GCGC^[CT]
2687 GGCGCT
MroI 189 TCCGGA T^CCGGA
819 TCCGGA
NsiI 1577 ATGCAT ATGCA^T
1982 ATGCAT
SbfI 1040 CCTGCAGG CCTGCA^GG
3822 CCTGCAGG
Sse8387I 1040 CCTGCAGG CCTGCA^GG
3822 CCTGCAGG
SspBI 258 TGTACA T^GTACA
708 TGTACA
XcmI 1477 CCAAAGGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1540 CCACAACACGGCTGG
XmnI 121 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3324 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII