##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AviII BbrPI BbsI BclI BfrI
BglII BpuAI BsaAI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2227 GGTACC G^GTACC
Aor51HI 618 AGCGCT AGC^GCT
Asp718I 2227 GGTACC G^GTACC
AvrII 3946 CCTAGG C^CTAGG
BbeI 3987 GGCGCC GGCGC^C
BciVI 2524 GTATCC GTATCC(6/5)
BlnI 3946 CCTAGG C^CTAGG
BsaBI 3142 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 460 CGGCCG CG[GA][CT]^CG
BsmI 2661 GAATGC GAATGC(1/-1)
BstXI 1780 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 3378 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1235 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 460 CGGCCG C^GGCCG
EclXI 460 CGGCCG C^GGCCG
Eco47III 618 AGCGCT AGC^GCT
Eco52I 460 CGGCCG C^GGCCG
FseI 457 GGCCGGCC GGCCGG^CC
KasI 3987 GGCGCC G^GCGCC
KpnI 2227 GGTACC GGTAC^C
MaeII 4104 ACGT A^CGT
MamI 3142 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 4175 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 3987 GGCGCC GG^CGCC
NcoI 1179 CCATGG C^CATGG
NspI 3017 ACATGC [GA]CATG^[CT]
SapI 593 GAAGAGC GCTCTTC(1/4)
SexAI 4011 ACCTGGT A^CC[AT]GGT
SfoI 3987 GGCGCC GGC^GCC
SmaI 2560 CCCGGG CCC^GGG
XmaCI 2560 CCCGGG C^CCGGG
XmaI 2560 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1321 AAATTC [GA]^AATT[CT]
2193 GAATTT
ApoI 1321 AAATTC [GA]^AATT[CT]
2193 GAATTT
AspI 95 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3798 GACAGGGTC
BamHI 785 GGATCC G^GATCC
4411 GGATCC
BlpI 255 GCTCAGC GC^T[ACGT]AGC
2256 GCTGAGC
Bpu1102I 255 GCTCAGC GC^T[ACGT]AGC
2256 GCTGAGC
BsaWI 2843 ACCGGA [AT]^CCGG[AT]
4095 ACCGGA
BsmBI 3993 CGTCTC CGTCTC(1/5)
3763 GAGACG
BstAPI 937 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2916 GCAGGCCCTGC
CelII 255 GCTCAGC GC^T[ACGT]AGC
2256 GCTGAGC
KspI 2359 CCGCGG CCGC^GG
2904 CCGCGG
MaeIII 121 GTGAC ^GT[ACGT]AC
4131 GTGAC
MseI 3545 TTAA T^TAA
4525 TTAA
PaeR7I 1103 CTCGAG C^TCGAG
2181 CTCGAG
PflFI 95 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3798 GACAGGGTC
SacII 2359 CCGCGG CCGC^GG
2904 CCGCGG
SfiI 406 GGCCAAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3075 GGCCCAGCGGGCC
Tru9I 3545 TTAA T^TAA
4525 TTAA
Tsp45I 121 GTGAC ^GT[GC]AC
4131 GTGAC
Tsp509I 1322 AATT ^AATT
2194 AATT
Tth111I 95 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3798 GACAGGGTC
XhoI 1103 CTCGAG C^TCGAG
2181 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI TaqI TfiI TseI TspRI
TthHB8I Van91I XcmI XhoII