##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII BbeI BbrPI
BbvCI BcgI BclI BlpI Bpu1102I
BsaAI BsaHI BsiEI BsiWI BsoBI
BspDI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 385 CGCG CG^CG
AccIII 3626 TCCGGA T^CCGGA
AclI 3711 AACGTT AA^CGTT
AflII 4425 CTTAAG C^TTAAG
AflIII 260 ACACGT A^C[GA][CT]GT
AocI 3426 CCTGAGG CC^T[ACGT]AGG
AspI 3857 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3229 GGATAC GTATCC(6/5)
BcnI 188 CCGGG CC^[GC]GG
BfrI 4425 CTTAAG C^TTAAG
BglI 2162 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2732 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3626 TCCGGA [AT]^CCGG[AT]
BseAI 3626 TCCGGA T^CCGGA
BsmBI 1666 CGTCTC CGTCTC(1/5)
BsmI 1967 GAATGC GAATGC(1/-1)
Bsp1407I 2000 TGTACA T^GTACA
BspEI 3626 TCCGGA T^CCGGA
BsrGI 2000 TGTACA T^GTACA
BstUI 385 CGCG CG^CG
Bsu36I 3426 CCTGAGG CC^T[ACGT]AGG
CfoI 386 GCGC GCG^C
DraIII 2014 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3426 CCTGAGG CC^T[ACGT]AGG
EcoNI 5 CCTAGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1290 GAATTC G^AATTC
EcoT22I 4494 ATGCAT ATGCA^T
HhaI 386 GCGC GCG^C
HinP1I 386 GCGC G^CGC
HindIII 687 AAGCTT A^AGCTT
MamI 2732 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3626 TCCGGA T^CCGGA
MvnI 385 CGCG CG^CG
NciI 188 CCGGG CC^[GC]GG
NsiI 4494 ATGCAT ATGCA^T
PflFI 3857 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 768 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3711 AACGTT AA^CGTT
SexAI 3826 ACCAGGT A^CC[AT]GGT
SphI 3750 GCATGC GCATG^C
SspBI 2000 TGTACA T^GTACA
StuI 3878 AGGCCT AGG^CCT
Tth111I 3857 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 768 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvrII 5 CCTAGG C^CTAGG
1579 CCTAGG
BamHI 96 GGATCC G^GATCC
613 GGATCC
BanI 1 GGCACC G^G[CT][GA]CC
1469 GGTGCC
BbsI 3997 GAAGAC GAAGAC(2/6)
2244 GTCTTC
BglII 4010 AGATCT A^GATCT
4161 AGATCT
BlnI 5 CCTAGG C^CTAGG
1579 CCTAGG
BpuAI 3997 GAAGAC GAAGAC(2/6)
2244 GTCTTC
BsaI 216 GAGACC GGTCTC(1/5)
2766 GGTCTC
BsgI 2108 CTGCAC GTGCAG(16/14)
1653 GTGCAG
BspHI 266 TCATGA T^CATGA
2722 TCATGA
BsrDI 1636 CATTGC GCAATG(2/0)
2114 CATTGC
BstXI 1094 CCATGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3252 CCACCTTCCTGG
DsaI 4240 CCATGG C^C[GA][CT]GG
4284 CCATGG
EcoRV 1121 GATATC GAT^ATC
2428 GATATC
HapII 188 CCGG C^CGG
3627 CCGG
HpaII 188 CCGG C^CGG
3627 CCGG
MspI 188 CCGG C^CGG
3627 CCGG
NcoI 4240 CCATGG C^CATGG
4284 CCATGG
PpuMI 415 AGGTCCT [GA]G^G[AT]CC[CT]
4003 AGGACCT
RcaI 266 TCATGA T^CATGA
2722 TCATGA
TaqI 2866 TCGA T^CGA
3664 TCGA
TthHB8I 2866 TCGA T^CGA
3664 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII