##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbsI BciVI BclI BfrI
BlnI BpuAI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI EcoRV
EcoT22I FbaI FspI HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
ScaI SfuI SnaBI SpeI SspBI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 4227 ACGTGT A^C[GA][CT]GT
AgeI 4083 ACCGGT A^CCGGT
Alw44I 4223 GTGCAC G^TGCAC
ApaLI 4223 GTGCAC G^TGCAC
AspI 1018 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4226 CACGTG CAC^GTG
BsaAI 4226 CACGTG [CT]AC^GT[GA]
BsaWI 4083 ACCGGT [AT]^CCGG[AT]
BsmBI 867 CGTCTC CGTCTC(1/5)
BssSI 403 CACGAG CACGAG(-5/-1)
BstEII 55 GGTGACC G^GT[ACGT]ACC
BstPI 55 GGTGACC G^GT[ACGT]ACC
DraI 4529 TTTAAA TTT^AAA
EcoO65I 55 GGTGACC G^GT[ACGT]ACC
HincII 3953 GTCGAC GT[CT]^[GA]AC
HindII 3953 GTCGAC GT[CT]^[GA]AC
HindIII 1157 AAGCTT A^AGCTT
MseI 4530 TTAA T^TAA
NspI 337 GCATGC [GA]CATG^[CT]
PflFI 1018 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4083 ACCGGT A^CCGGT
PmaCI 4226 CACGTG CAC^GTG
PmlI 4226 CACGTG CAC^GTG
SalI 3953 GTCGAC G^TCGAC
SexAI 126 ACCTGGT A^CC[AT]GGT
SfiI 3928 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3394 CGCCGGCG C[GA]^CCGG[CT]G
SphI 337 GCATGC GCATG^C
StuI 4216 AGGCCT AGG^CCT
Tru9I 4530 TTAA T^TAA
Tth111I 1018 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1162 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 41 GTCTAC GT^[AC][GT]AC
3953 GTCGAC
AhdI 1700 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2426 GACCCCTCGTC
AocI 440 CCTGAGG CC^T[ACGT]AGG
2180 CCTCAGG
AspEI 1700 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2426 GACCCCTCGTC
BcgI 4333 CGAGACCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2941 GCAGGACAGTCG
BglII 1410 AGATCT A^GATCT
1587 AGATCT
BsmI 2101 GCATTC GAATGC(1/-1)
2346 GCATTC
BssHII 1470 GCGCGC G^CGCGC
2257 GCGCGC
Bsu36I 440 CCTGAGG CC^T[ACGT]AGG
2180 CCTCAGG
DraIII 50 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2066 CACACGGTG
DrdI 3944 GACCGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4078 GACGCACCGGTC
Eam1105I 1700 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2426 GACCCCTCGTC
Eco81I 440 CCTGAGG CC^T[ACGT]AGG
2180 CCTCAGG
PpuMI 2184 AGGACCC [GA]G^G[AT]CC[CT]
2636 GGGTCCC
SbfI 1231 CCTGCAGG CCTGCA^GG
4141 CCTGCAGG
SmaI 3622 CCCGGG CCC^GGG
3939 CCCGGG
Sse8387I 1231 CCTGCAGG CCTGCA^GG
4141 CCTGCAGG
XmaCI 3622 CCCGGG C^CCGGG
3939 CCCGGG
XmaI 3622 CCCGGG C^CCGGG
3939 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FseI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII