##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI AscI AseI AsnI Asp718I
AspEI AspI BbeI BcgI BciVI
BfrI BglII BsiWI BspDI BspLU11I
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRV HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1592 GTCTAC GT^[AC][GT]AC
AccIII 1854 TCCGGA T^CCGGA
AcyI 2642 GACGCC G[GA]^CG[CT]C
AgeI 33 ACCGGT A^CCGGT
Aor51HI 1230 AGCGCT AGC^GCT
AviII 816 TGCGCA TGC^GCA
AvrII 2661 CCTAGG C^CTAGG
BbrPI 273 CACGTG CAC^GTG
BclI 3043 TGATCA T^GATCA
BlnI 2661 CCTAGG C^CTAGG
BsaBI 2840 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2642 GACGCC G[GA]^CG[CT]C
BseAI 1854 TCCGGA T^CCGGA
BsiEI 517 CGGTCG CG[GA][CT]^CG
BsmBI 3409 GAGACG CGTCTC(1/5)
BspEI 1854 TCCGGA T^CCGGA
BsrDI 3594 CATTGC GCAATG(2/0)
BssHII 3900 GCGCGC G^CGCGC
Eco47III 1230 AGCGCT AGC^GCT
EcoRI 3908 GAATTC G^AATTC
FbaI 3043 TGATCA T^GATCA
FseI 885 GGCCGGCC GGCCGG^CC
FspI 816 TGCGCA TGC^GCA
Hin1I 2642 GACGCC G[GA]^CG[CT]C
HincII 2462 GTTGAC GT[CT]^[GA]AC
HindII 2462 GTTGAC GT[CT]^[GA]AC
MamI 2840 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1854 TCCGGA T^CCGGA
MseI 4322 TTAA T^TAA
NcoI 3860 CCATGG C^CATGG
PaeR7I 3631 CTCGAG C^TCGAG
PinAI 33 ACCGGT A^CCGGT
PmaCI 273 CACGTG CAC^GTG
PmlI 273 CACGTG CAC^GTG
ScaI 163 AGTACT AGT^ACT
SpeI 2015 ACTAGT A^CTAGT
Tru9I 4322 TTAA T^TAA
XbaI 3023 TCTAGA T^CTAGA
XhoI 3631 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 3899 CGCG CG^CG
3901 CGCG
Alw44I 638 GTGCAC G^TGCAC
2221 GTGCAC
ApaI 1336 GGGCCC GGGCC^C
3210 GGGCCC
ApaLI 638 GTGCAC G^TGCAC
2221 GTGCAC
Asp700I 758 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3032 GAAAGTGTTC
BamHI 620 GGATCC G^GATCC
3239 GGATCC
BbvCI 2169 CCTCAGC CCTCAGC(-5/-2)
452 GCTGAGG
BglI 417 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
913 GCCGGATGGGC
Bsp1407I 1080 TGTACA T^GTACA
4428 TGTACA
BsrBI 1192 GAGCGG CCGCTC(-3/-3)
3565 GAGCGG
BsrGI 1080 TGTACA T^GTACA
4428 TGTACA
BstAPI 1530 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2810 GCATTGAATGC
BstUI 3899 CGCG CG^CG
3901 CGCG
DraIII 1488 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3735 CACCGAGTG
EcoT22I 2808 ATGCAT ATGCA^T
2817 ATGCAT
HgaI 2642 GACGC GACGC(5/10)
2652 GCGTC
MvnI 3899 CGCG CG^CG
3901 CGCG
NsiI 2808 ATGCAT ATGCA^T
2817 ATGCAT
SapI 4109 GAAGAGC GCTCTTC(1/4)
3537 GCTCTTC
SmaI 337 CCCGGG CCC^GGG
1339 CCCGGG
SphI 1945 GCATGC GCATG^C
2859 GCATGC
SspBI 1080 TGTACA T^GTACA
4428 TGTACA
XmaCI 337 CCCGGG C^CCGGG
1339 CCCGGG
XmaI 337 CCCGGG C^CCGGG
1339 CCCGGG
XmnI 758 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3032 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII