##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccIII AclI AflIII Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AviII AvrII BalI BbeI
BbrPI BclI BlnI BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HpaI KasI
KpnI MamI MluI MluNI MroI
MscI NarI NdeI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2452 GACGTC GACGT^C
AccI 3344 GTCGAC GT^[AC][GT]AC
AcyI 2452 GACGTC G[GA]^CG[CT]C
AgeI 2205 ACCGGT A^CCGGT
AhdI 3881 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 553 CCTTAGG CC^T[ACGT]AGG
AspEI 3881 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1128 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 182 GGATCC G^GATCC
BciVI 386 GGATAC GTATCC(6/5)
BglI 1539 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2611 CACGTA [CT]AC^GT[GA]
BsaHI 2452 GACGTC G[GA]^CG[CT]C
BsaWI 2205 ACCGGT [AT]^CCGG[AT]
BspHI 1843 TCATGA T^CATGA
BsrBI 1160 CCGCTC CCGCTC(-3/-3)
BstEII 2201 GGTAACC G^GT[ACGT]ACC
BstPI 2201 GGTAACC G^GT[ACGT]ACC
Bsu36I 553 CCTTAGG CC^T[ACGT]AGG
DraIII 1049 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 469 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3881 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 553 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2201 GGTAACC G^GT[ACGT]ACC
HaeII 26 AGCGCC [GA]GCGC^[CT]
Hin1I 2452 GACGTC G[GA]^CG[CT]C
HindIII 3801 AAGCTT A^AGCTT
KspI 2715 CCGCGG CCGC^GG
MfeI 1439 CAATTG C^AATTG
MunI 1439 CAATTG C^AATTG
NaeI 2530 GCCGGC GCC^GGC
NgoMIV 2530 GCCGGC G^CCGGC
PaeR7I 1634 CTCGAG C^TCGAG
PflFI 1128 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1853 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2205 ACCGGT A^CCGGT
RcaI 1843 TCATGA T^CATGA
SacI 1316 GAGCTC GAGCT^C
SacII 2715 CCGCGG CCGC^GG
SalI 3344 GTCGAC G^TCGAC
SapI 408 GCTCTTC GCTCTTC(1/4)
SphI 1886 GCATGC GCATG^C
Tth111I 1128 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1853 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1634 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 2253 ATTAAT AT^TAAT
4090 ATTAAT
AsnI 2253 ATTAAT AT^TAAT
4090 ATTAAT
AvaI 852 CCCGAG C^[CT]CG[GA]G
1634 CTCGAG
BbsI 112 GAAGAC GAAGAC(2/6)
3104 GTCTTC
BcgI 3346 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3307 GCACTGCGCTCG
BcnI 1675 CCCGG CC^[GC]GG
3303 CCCGG
BglII 1764 AGATCT A^GATCT
2817 AGATCT
BlpI 2671 GCTAAGC GC^T[ACGT]AGC
3989 GCTCAGC
Bpu1102I 2671 GCTAAGC GC^T[ACGT]AGC
3989 GCTCAGC
BpuAI 112 GAAGAC GAAGAC(2/6)
3104 GTCTTC
BsoBI 852 CCCGAG C^[CT]CG[GA]G
1634 CTCGAG
CelII 2671 GCTAAGC GC^T[ACGT]AGC
3989 GCTCAGC
EaeI 1446 TGGCCG [CT]^GGCC[GA]
3261 CGGCCA
EarI 409 CTCTTC CTCTTC(1/4)
2345 CTCTTC
Ksp632I 409 CTCTTC CTCTTC(1/4)
2345 CTCTTC
NciI 1675 CCCGG CC^[GC]GG
3303 CCCGG
PpuMI 476 AGGTCCT [GA]G^G[AT]CC[CT]
1516 AGGTCCT
PshBI 2253 ATTAAT AT^TAAT
4090 ATTAAT
ScaI 1217 AGTACT AGT^ACT
1405 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbvCI BbvI
BfaI BfrI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII