##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AviII AvrII BciVI BlnI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HpaI MluI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3181 GGTACC G^GTACC
AccIII 1313 TCCGGA T^CCGGA
AflII 1242 CTTAAG C^TTAAG
Asp700I 3035 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3181 GGTACC G^GTACC
BamHI 3500 GGATCC G^GATCC
BbeI 1186 GGCGCC GGCGC^C
BbsI 2982 GAAGAC GAAGAC(2/6)
BfrI 1242 CTTAAG C^TTAAG
BglII 3488 AGATCT A^GATCT
BpuAI 2982 GAAGAC GAAGAC(2/6)
BsaBI 3332 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2317 GGTCTC GGTCTC(1/5)
BseAI 1313 TCCGGA T^CCGGA
BsmI 302 GCATTC GAATGC(1/-1)
BspEI 1313 TCCGGA T^CCGGA
BspLU11I 2449 ACATGT A^CATGT
BsrBI 66 GAGCGG CCGCTC(-3/-3)
BsrFI 1012 GCCGGC [GA]^CCGG[CT]
BssSI 1610 CACGAG CACGAG(-5/-1)
Cfr10I 1012 GCCGGC [GA]^CCGG[CT]
DraI 1837 TTTAAA TTT^AAA
EarI 1670 CTCTTC CTCTTC(1/4)
EcoRI 1335 GAATTC G^AATTC
EcoT22I 1362 ATGCAT ATGCA^T
HindIII 1543 AAGCTT A^AGCTT
KasI 1186 GGCGCC G^GCGCC
KpnI 3181 GGTACC GGTAC^C
Ksp632I 1670 CTCTTC CTCTTC(1/4)
KspI 405 CCGCGG CCGC^GG
MamI 3332 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1172 CAATTG C^AATTG
MroI 1313 TCCGGA T^CCGGA
MunI 1172 CAATTG C^AATTG
NaeI 1012 GCCGGC GCC^GGC
NarI 1186 GGCGCC GG^CGCC
NgoMIV 1012 GCCGGC G^CCGGC
NheI 3935 GCTAGC G^CTAGC
NsiI 1362 ATGCAT ATGCA^T
SacII 405 CCGCGG CCGC^GG
SbfI 123 CCTGCAGG CCTGCA^GG
SexAI 1870 ACCTGGT A^CC[AT]GGT
SfoI 1186 GGCGCC GGC^GCC
Sse8387I 123 CCTGCAGG CCTGCA^GG
SspI 2973 AATATT AAT^ATT
XbaI 3650 TCTAGA T^CTAGA
XmnI 3035 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2640 GTCTAC GT^[AC][GT]AC
4018 GTAGAC
AcsI 1335 GAATTC [GA]^AATT[CT]
3975 GAATTT
AflIII 2449 ACATGT A^C[GA][CT]GT
4145 ACACGT
Alw44I 1121 GTGCAC G^TGCAC
1482 GTGCAC
ApaLI 1121 GTGCAC G^TGCAC
1482 GTGCAC
ApoI 1335 GAATTC [GA]^AATT[CT]
3975 GAATTT
BbrPI 1479 CACGTG CAC^GTG
4146 CACGTG
BcgI 135 CGACGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
927 CGACCAGGGTGC
BclI 2709 TGATCA T^GATCA
3864 TGATCA
BglI 145 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
485 GCCTCCAGGGC
BsaAI 1479 CACGTG [CT]AC^GT[GA]
4146 CACGTG
BsaWI 548 ACCGGA [AT]^CCGG[AT]
1313 TCCGGA
BsmBI 3296 CGTCTC CGTCTC(1/5)
2287 GAGACG
BspHI 3340 TCATGA T^CATGA
3422 TCATGA
BstAPI 6 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
15 GCATGAGCTGC
EcoRV 3117 GATATC GAT^ATC
3155 GATATC
FbaI 2709 TGATCA T^GATCA
3864 TGATCA
HincII 376 GTCAAC GT[CT]^[GA]AC
1132 GTCAAC
HindII 376 GTCAAC GT[CT]^[GA]AC
1132 GTCAAC
MaeII 1480 ACGT A^CGT
4147 ACGT
NspI 2449 ACATGT [GA]CATG^[CT]
3266 ACATGC
PmaCI 1479 CACGTG CAC^GTG
4146 CACGTG
PmlI 1479 CACGTG CAC^GTG
4146 CACGTG
PpuMI 251 AGGACCC [GA]G^G[AT]CC[CT]
1954 GGGACCC
RcaI 3340 TCATGA T^CATGA
3422 TCATGA
SacI 646 GAGCTC GAGCT^C
1664 GAGCTC
ScaI 2365 AGTACT AGT^ACT
3670 AGTACT
SmaI 938 CCCGGG CCC^GGG
2193 CCCGGG
XcmI 362 CCAGATCCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2847 CCACAGCTTCCATGG
XmaCI 938 CCCGGG C^CCGGG
2193 CCCGGG
XmaI 938 CCCGGG C^CCGGG
2193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII