##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AviII BamHI BcgI BclI BfrI
BglII BlpI Bpu1102I BsiWI BspDI
BspLU11I BsrDI Bst1107I BstBI BstYI
BstZ17I CelII ClaI DraI DrdI
Eco47III EcoRV FbaI FspI HindIII
HpaI MflI MluI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 3572 TCCGGA T^CCGGA
AclI 2740 AACGTT AA^CGTT
AflIII 1116 ACACGT A^C[GA][CT]GT
AhdI 3863 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3863 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3838 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1035 TGGCCA TGG^CCA
BbeI 21 GGCGCC GGCGC^C
BbrPI 2992 CACGTG CAC^GTG
BbvCI 236 CCTCAGC CCTCAGC(-5/-2)
BglI 70 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2992 CACGTG [CT]AC^GT[GA]
BsaBI 457 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3572 TCCGGA [AT]^CCGG[AT]
BseAI 3572 TCCGGA T^CCGGA
BspEI 3572 TCCGGA T^CCGGA
BspHI 1575 TCATGA T^CATGA
BssHII 3489 GCGCGC G^CGCGC
BssSI 1418 CACGAG CACGAG(-5/-1)
BstAPI 3763 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4298 GGTGACC G^GT[ACGT]ACC
BstPI 4298 GGTGACC G^GT[ACGT]ACC
CpoI 3861 CGGACCG CG^G[AT]CCG
DraIII 3439 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3863 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4298 GGTGACC G^GT[ACGT]ACC
FseI 100 GGCCGGCC GGCCGG^CC
HaeII 21 GGCGCC [GA]GCGC^[CT]
HincII 946 GTCAAC GT[CT]^[GA]AC
HindII 946 GTCAAC GT[CT]^[GA]AC
KasI 21 GGCGCC G^GCGCC
KspI 106 CCGCGG CCGC^GG
MamI 457 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2295 CAATTG C^AATTG
MluNI 1035 TGGCCA TGG^CCA
MroI 3572 TCCGGA T^CCGGA
MscI 1035 TGGCCA TGG^CCA
MunI 2295 CAATTG C^AATTG
NarI 21 GGCGCC GG^CGCC
PflFI 3838 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2614 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2992 CACGTG CAC^GTG
PmlI 2992 CACGTG CAC^GTG
Psp1406I 2740 AACGTT AA^CGTT
RcaI 1575 TCATGA T^CATGA
RsrII 3861 CGGACCG CG^G[AT]CCG
SacI 4242 GAGCTC GAGCT^C
SacII 106 CCGCGG CCGC^GG
ScaI 1428 AGTACT AGT^ACT
SfoI 21 GGCGCC GGC^GCC
Tth111I 3838 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2614 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3376 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 4160 GGTACC G^GTACC
4391 GGTACC
AccII 107 CGCG CG^CG
3490 CGCG
AcyI 21 GGCGCC G[GA]^CG[CT]C
1215 GGCGTC
Asp700I 616 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3470 GAACTCTTTC
Asp718I 4160 GGTACC G^GTACC
4391 GGTACC
AvaI 2643 CTCGGG C^[CT]CG[GA]G
3957 CTCGGG
AvrII 2215 CCTAGG C^CTAGG
4120 CCTAGG
BbsI 616 GAAGAC GAAGAC(2/6)
2428 GAAGAC
BlnI 2215 CCTAGG C^CTAGG
4120 CCTAGG
BpuAI 616 GAAGAC GAAGAC(2/6)
2428 GAAGAC
BsaHI 21 GGCGCC G[GA]^CG[CT]C
1215 GGCGTC
BsaI 2011 GAGACC GGTCTC(1/5)
3119 GAGACC
BsmBI 3111 CGTCTC CGTCTC(1/5)
57 GAGACG
BsoBI 2643 CTCGGG C^[CT]CG[GA]G
3957 CTCGGG
BstUI 107 CGCG CG^CG
3490 CGCG
EcoRI 231 GAATTC G^AATTC
1885 GAATTC
EcoT22I 1157 ATGCAT ATGCA^T
2747 ATGCAT
Hin1I 21 GGCGCC G[GA]^CG[CT]C
1215 GGCGTC
KpnI 4160 GGTACC GGTAC^C
4391 GGTACC
MvnI 107 CGCG CG^CG
3490 CGCG
NaeI 46 GCCGGC GCC^GGC
101 GCCGGC
NcoI 330 CCATGG C^CATGG
1330 CCATGG
NgoMIV 46 GCCGGC G^CCGGC
101 GCCGGC
NsiI 1157 ATGCAT ATGCA^T
2747 ATGCAT
NspI 2255 GCATGT [GA]CATG^[CT]
2837 ACATGC
PpuMI 2024 AGGTCCT [GA]G^G[AT]CC[CT]
2184 GGGTCCC
SapI 835 GAAGAGC GCTCTTC(1/4)
3127 GCTCTTC
XcmI 330 CCATGGAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
768 CCATTTCAGAGATGG
XmnI 616 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3470 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaII BanI BanII BbvI BciVI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I