##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp718I
AvrII BamHI BbrPI BclI BfrI
BlnI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssSI Bst1107I BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV FbaI FseI
HindIII HpaI KpnI MfeI MroI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TthHB8I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1880 GTCTAC GT^[AC][GT]AC
AflIII 1632 ACGCGT A^C[GA][CT]GT
AgeI 211 ACCGGT A^CCGGT
AhdI 935 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1897 GTGCAC G^TGCAC
ApaLI 1897 GTGCAC G^TGCAC
AspEI 935 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2935 CGATCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1923 AGATCT A^GATCT
BsaAI 2531 TACGTG [CT]AC^GT[GA]
BsaBI 2088 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 44 GAATGC GAATGC(1/-1)
Bsp1407I 993 TGTACA T^GTACA
BspHI 522 TCATGA T^CATGA
BsrGI 993 TGTACA T^GTACA
BssHII 733 GCGCGC G^CGCGC
BstAPI 3126 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1276 GGTGACC G^GT[ACGT]ACC
BstPI 1276 GGTGACC G^GT[ACGT]ACC
DraI 3909 TTTAAA TTT^AAA
Eam1105I 935 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1276 GGTGACC G^GT[ACGT]ACC
EcoRI 3181 GAATTC G^AATTC
EcoT22I 3855 ATGCAT ATGCA^T
HincII 4 GTTGAC GT[CT]^[GA]AC
HindII 4 GTTGAC GT[CT]^[GA]AC
KspI 909 CCGCGG CCGC^GG
MamI 2088 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1632 ACGCGT A^CGCGT
NaeI 2052 GCCGGC GCC^GGC
NgoMIV 2052 GCCGGC G^CCGGC
NsiI 3855 ATGCAT ATGCA^T
PinAI 211 ACCGGT A^CCGGT
RcaI 522 TCATGA T^CATGA
SacII 909 CCGCGG CCGC^GG
SgrAI 2051 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 993 TGTACA T^GTACA
XcmI 2989 CCAGGCCCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspI 2640 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3108 GACCTGGTC
AviII 1409 TGCGCA TGC^GCA
3583 TGCGCA
BalI 2220 TGGCCA TGG^CCA
2308 TGGCCA
BciVI 2168 GGATAC GTATCC(6/5)
1368 GTATCC
BsaWI 211 ACCGGT [AT]^CCGG[AT]
483 ACCGGA
DraIII 1094 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2657 CACCCAGTG
DrdI 325 GACTCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1964 GACACCACAGTC
FspI 1409 TGCGCA TGC^GCA
3583 TGCGCA
MaeII 828 ACGT A^CGT
2532 ACGT
MluNI 2220 TGGCCA TGG^CCA
2308 TGGCCA
MscI 2220 TGGCCA TGG^CCA
2308 TGGCCA
NcoI 1374 CCATGG C^CATGG
3665 CCATGG
PflFI 2640 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3108 GACCTGGTC
SapI 574 GCTCTTC GCTCTTC(1/4)
2817 GCTCTTC
SexAI 1359 ACCTGGT A^CC[AT]GGT
3109 ACCTGGT
SmlI 363 CTCAAG C^T[CT][GA]AG
1847 CTCAAG
Tth111I 2640 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3108 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII XmaCI
XmaI XmnI