##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BcgI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstBI BstUI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT14I EcoT22I FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StyI SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 2938 AACGTT AA^CGTT
AcyI 247 GGCGTC G[GA]^CG[CT]C
AflII 3011 CTTAAG C^TTAAG
AvaI 4670 CCCGGG C^[CT]CG[GA]G
BalI 4879 TGGCCA TGG^CCA
BamHI 1908 GGATCC G^GATCC
BclI 297 TGATCA T^GATCA
BfrI 3011 CTTAAG C^TTAAG
BglII 2301 AGATCT A^GATCT
BsaHI 247 GGCGTC G[GA]^CG[CT]C
BsmBI 283 GAGACG CGTCTC(1/5)
BsoBI 4670 CCCGGG C^[CT]CG[GA]G
Bst1107I 1570 GTATAC GTA^TAC
BstEII 2414 GGTAACC G^GT[ACGT]ACC
BstPI 2414 GGTAACC G^GT[ACGT]ACC
BstZ17I 1570 GTATAC GTA^TAC
DraIII 4817 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4784 CCGTGG C^C[GA][CT]GG
EcoO65I 2414 GGTAACC G^GT[ACGT]ACC
EcoRV 3758 GATATC GAT^ATC
FauI 4851 GCGGG CCCGC(4/6)
FbaI 297 TGATCA T^GATCA
Hin1I 247 GGCGTC G[GA]^CG[CT]C
MluNI 4879 TGGCCA TGG^CCA
MscI 4879 TGGCCA TGG^CCA
MspA1I 3259 CAGCGG C[AC]G^C[GT]G
NdeI 203 CATATG CA^TATG
Psp1406I 2938 AACGTT AA^CGTT
SacI 1699 GAGCTC GAGCT^C
SalI 2213 GTCGAC G^TCGAC
SmaI 4670 CCCGGG CCC^GGG
SphI 642 GCATGC GCATG^C
SspI 1792 AATATT AAT^ATT
StuI 4205 AGGCCT AGG^CCT
XmaCI 4670 CCCGGG C^CCGGG
XmaI 4670 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1570 GTATAC GT^[AC][GT]AC
2213 GTCGAC
Alw44I 2507 GTGCAC G^TGCAC
2641 GTGCAC
ApaLI 2507 GTGCAC G^TGCAC
2641 GTGCAC
BbvCI 1672 CCTCAGC CCTCAGC(-5/-2)
4710 GCTGAGG
BcnI 4670 CCCGG CC^[GC]GG
4671 CCGGG
BglI 2390 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2471 GCCCAATGGGC
BsaAI 3125 CACGTA [CT]AC^GT[GA]
5403 TACGTG
BsaBI 2050 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4648 GATGTGGATC
BsmI 3327 GAATGC GAATGC(1/-1)
4873 GCATTC
BspLU11I 1283 ACATGT A^CATGT
3373 ACATGT
BstXI 717 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2220 CCAGAAGACTGG
EaeI 2397 TGGCCG [CT]^GGCC[GA]
4879 TGGCCA
EcoNI 2667 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3189 CCTCAGTGAGG
EcoRI 421 GAATTC G^AATTC
3955 GAATTC
MamI 2050 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4648 GATGTGGATC
NciI 4670 CCCGG CC^[GC]GG
4671 CCGGG
PpuMI 771 AGGACCT [GA]G^G[AT]CC[CT]
3212 AGGTCCC
SapI 4772 GAAGAGC GCTCTTC(1/4)
4953 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI