##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AgeI Aor51HI AseI
AsnI AviII BbeI BciVI BglII
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eco47III
EcoT22I FseI FspI KasI KspI
MamI MroI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI PvuI RsrII
SacII SbfI ScaI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1016 GGTACC G^GTACC
AccI 2146 GTCGAC GT^[AC][GT]AC
AclI 2249 AACGTT AA^CGTT
AhdI 2267 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 1176 GGCGCGCC GG^CGCGCC
Asp718I 1016 GGTACC G^GTACC
AspEI 2267 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4290 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 596 GGATCC G^GATCC
BbvCI 3345 CCTCAGC CCTCAGC(-5/-2)
BcgI 1046 GCACGGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3900 TGATCA T^GATCA
BspHI 5362 TCATGA T^CATGA
BspLU11I 5459 ACATGT A^CATGT
BsrDI 4105 CATTGC GCAATG(2/0)
BssHII 1177 GCGCGC G^CGCGC
BstXI 665 CCACCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 422 CGGCCG C^GGCCG
Eam1105I 2267 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 422 CGGCCG C^GGCCG
Eco52I 422 CGGCCG C^GGCCG
EcoRI 1971 GAATTC G^AATTC
EcoRV 3279 GATATC GAT^ATC
FbaI 3900 TGATCA T^GATCA
HpaI 2170 GTTAAC GTT^AAC
KpnI 1016 GGTACC GGTAC^C
MfeI 4409 CAATTG C^AATTG
MluI 3190 ACGCGT A^CGCGT
MunI 4409 CAATTG C^AATTG
NaeI 424 GCCGGC GCC^GGC
NcoI 2384 CCATGG C^CATGG
NgoMIV 424 GCCGGC G^CCGGC
NheI 562 GCTAGC G^CTAGC
PaeR7I 3138 CTCGAG C^TCGAG
PflFI 4290 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2249 AACGTT AA^CGTT
RcaI 5362 TCATGA T^CATGA
SalI 2146 GTCGAC G^TCGAC
SexAI 2061 ACCAGGT A^CC[AT]GGT
SpeI 2166 ACTAGT A^CTAGT
SphI 4414 GCATGC GCATG^C
Tth111I 4290 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1717 TCTAGA T^CTAGA
XhoI 3138 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1565 CTTAAG C^TTAAG
5331 CTTAAG
Asp700I 1967 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4232 GAACTCCTTC
BbsI 448 GTCTTC GAAGAC(2/6)
2768 GTCTTC
BfrI 1565 CTTAAG C^TTAAG
5331 CTTAAG
BlpI 3143 GCTCAGC GC^T[ACGT]AGC
4598 GCTCAGC
Bpu1102I 3143 GCTCAGC GC^T[ACGT]AGC
4598 GCTCAGC
BpuAI 448 GTCTTC GAAGAC(2/6)
2768 GTCTTC
BsiEI 422 CGGCCG CG[GA][CT]^CG
2831 CGACCG
BsmBI 345 CGTCTC CGTCTC(1/5)
2032 GAGACG
BsmI 4736 GAATGC GAATGC(1/-1)
2894 GCATTC
BsrBI 393 CCGCTC CCGCTC(-3/-3)
45 GAGCGG
BsrFI 35 GCCGGT [GA]^CCGG[CT]
424 GCCGGC
BstAPI 1427 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1811 GCAGCCTGTGC
BstEII 336 GGTCACC G^GT[ACGT]ACC
546 GGTCACC
BstPI 336 GGTCACC G^GT[ACGT]ACC
546 GGTCACC
CelII 3143 GCTCAGC GC^T[ACGT]AGC
4598 GCTCAGC
Cfr10I 35 GCCGGT [GA]^CCGG[CT]
424 GCCGGC
DraI 3735 TTTAAA TTT^AAA
4495 TTTAAA
DraIII 1525 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
3157 CACTGGGTG
DsaI 144 CCGTGG C^C[GA][CT]GG
2384 CCATGG
EcoO65I 336 GGTCACC G^GT[ACGT]ACC
546 GGTCACC
HindIII 3426 AAGCTT A^AGCTT
3511 AAGCTT
SapI 225 GCTCTTC GCTCTTC(1/4)
5081 GCTCTTC
XmnI 1967 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4232 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbrPI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII