##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AocI AscI
Asp718I AspI BamHI BfrI BglII
BsaBI BseAI BsiWI BspEI BspHI
BspLU11I BsrBI Bst1107I BstEII BstPI
BstZ17I Bsu36I CpoI DraI Eco81I
EcoO65I EcoT22I FseI KpnI KspI
MamI MfeI MluI MroI MunI
NheI NotI NruI NsiI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AviII 3596 TGCGCA TGC^GCA
AvrII 5569 CCTAGG C^CTAGG
BbeI 3949 GGCGCC GGCGC^C
BbsI 5368 GTCTTC GAAGAC(2/6)
BciVI 1255 GTATCC GTATCC(6/5)
BclI 2681 TGATCA T^GATCA
BlnI 5569 CCTAGG C^CTAGG
BlpI 1938 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1938 GCTCAGC GC^T[ACGT]AGC
BpuAI 5368 GTCTTC GAAGAC(2/6)
BsaWI 89 TCCGGT [AT]^CCGG[AT]
BsmBI 1860 CGTCTC CGTCTC(1/5)
BsrFI 3824 GCCGGC [GA]^CCGG[CT]
BssHII 3932 GCGCGC G^CGCGC
BstBI 3679 TTCGAA TT^CGAA
BstYI 4889 GGATCT [GA]^GATC[CT]
CelII 1938 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3824 GCCGGC [GA]^CCGG[CT]
DrdI 4359 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 151 GAATTC G^AATTC
EcoRV 1436 GATATC GAT^ATC
FbaI 2681 TGATCA T^GATCA
FspI 3596 TGCGCA TGC^GCA
HpaI 2236 GTTAAC GTT^AAC
KasI 3949 GGCGCC G^GCGCC
MflI 4889 GGATCT [GA]^GATC[CT]
NaeI 3824 GCCGGC GCC^GGC
NarI 3949 GGCGCC GG^CGCC
NdeI 5176 CATATG CA^TATG
NgoMIV 3824 GCCGGC G^CCGGC
NspV 3679 TTCGAA TT^CGAA
PacI 1010 TTAATTAA TTAAT^TAA
PshAI 4266 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3724 GAGCTC GAGCT^C
ScaI 3257 AGTACT AGT^ACT
SexAI 4575 ACCTGGT A^CC[AT]GGT
SfoI 3949 GGCGCC GGC^GCC
SfuI 3679 TTCGAA TT^CGAA
SpeI 2138 ACTAGT A^CTAGT
SphI 4905 GCATGC GCATG^C
XbaI 5634 TCTAGA T^CTAGA
XhoII 4889 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 3501 GGCGTC G[GA]^CG[CT]C
3949 GGCGCC
AhdI 3385 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5083 GACCAGCGGTC
ApaI 36 GGGCCC GGGCC^C
4405 GGGCCC
Asp700I 873 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1062 GAACCTTTTC
AspEI 3385 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5083 GACCAGCGGTC
AvaI 1769 CCCGAG C^[CT]CG[GA]G
3559 CTCGGG
BbrPI 3904 CACGTG CAC^GTG
4053 CACGTG
BbvCI 3140 CCTCAGC CCTCAGC(-5/-2)
3376 GCTGAGG
BsaHI 3501 GGCGTC G[GA]^CG[CT]C
3949 GGCGCC
BsmI 3062 GAATGC GAATGC(1/-1)
2301 GCATTC
BsoBI 1769 CCCGAG C^[CT]CG[GA]G
3559 CTCGGG
BspDI 325 ATCGAT AT^CGAT
4858 ATCGAT
BstAPI 3481 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4905 GCATGCCGTGC
ClaI 325 ATCGAT AT^CGAT
4858 ATCGAT
Eam1105I 3385 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5083 GACCAGCGGTC
EcoNI 306 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4452 CCTGGAGAAGG
Hin1I 3501 GGCGTC G[GA]^CG[CT]C
3949 GGCGCC
PmaCI 3904 CACGTG CAC^GTG
4053 CACGTG
PmlI 3904 CACGTG CAC^GTG
4053 CACGTG
XmnI 873 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1062 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AseI AsnI AspHI AvaII
BalI BanI BanII BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshBI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI