##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AgeI AscI AseI AsnI
Asp700I Asp718I AvrII BciVI BglII
BlnI BspDI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EcoT22I
FseI KpnI KspI MluI NdeI
NotI NruI NsiI NspV PacI
PflMI PinAI PmeI PshBI PvuI
RsrII SacII SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI Van91I XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3305 GACGTC GACGT^C
AclI 1675 AACGTT AA^CGTT
AflII 4194 CTTAAG C^TTAAG
ApaI 1139 GGGCCC GGGCC^C
AviII 4936 TGCGCA TGC^GCA
BamHI 1921 GGATCC G^GATCC
BbeI 671 GGCGCC GGCGC^C
BclI 5370 TGATCA T^GATCA
BfrI 4194 CTTAAG C^TTAAG
BglI 391 GCCACGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2051 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2051 GCTTAGC GC^T[ACGT]AGC
BsaBI 2477 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 385 CGTACG C^GTACG
BsrDI 4625 GCAATG GCAATG(2/0)
BsrFI 366 GCCGGC [GA]^CCGG[CT]
BssHII 528 GCGCGC G^CGCGC
BstAPI 5792 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2051 GCTTAGC GC^T[ACGT]AGC
Cfr10I 366 GCCGGC [GA]^CCGG[CT]
DraIII 3526 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 293 CGGCCG C^GGCCG
EclXI 293 CGGCCG C^GGCCG
Eco52I 293 CGGCCG C^GGCCG
EcoRV 1074 GATATC GAT^ATC
FbaI 5370 TGATCA T^GATCA
FspI 4936 TGCGCA TGC^GCA
HpaI 2527 GTTAAC GTT^AAC
KasI 671 GGCGCC G^GCGCC
MamI 2477 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4322 CAATTG C^AATTG
MunI 4322 CAATTG C^AATTG
NaeI 366 GCCGGC GCC^GGC
NarI 671 GGCGCC GG^CGCC
NgoMIV 366 GCCGGC G^CCGGC
NheI 2718 GCTAGC G^CTAGC
PaeR7I 2469 CTCGAG C^TCGAG
PshAI 5383 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1675 AACGTT AA^CGTT
SalI 819 GTCGAC G^TCGAC
SapI 5748 GCTCTTC GCTCTTC(1/4)
SfoI 671 GGCGCC GGC^GCC
SpeI 1427 ACTAGT A^CTAGT
StuI 634 AGGCCT AGG^CCT
XcmI 9 CCATCCCACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2469 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 1012 TCCGGA T^CCGGA
5753 TCCGGA
AhdI 329 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
348 GACTCCTGGTC
Alw44I 1504 GTGCAC G^TGCAC
5305 GTGCAC
Aor51HI 311 AGCGCT AGC^GCT
610 AGCGCT
ApaLI 1504 GTGCAC G^TGCAC
5305 GTGCAC
AspEI 329 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
348 GACTCCTGGTC
AspI 3815 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4926 GACCCTGTC
BalI 283 TGGCCA TGG^CCA
3883 TGGCCA
BbrPI 1239 CACGTG CAC^GTG
4399 CACGTG
BcgI 811 GCAGGGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5766 GCAGGAGAATCG
BseAI 1012 TCCGGA T^CCGGA
5753 TCCGGA
BsiEI 293 CGGCCG CG[GA][CT]^CG
788 CGACCG
Bsp1407I 421 TGTACA T^GTACA
1025 TGTACA
BspEI 1012 TCCGGA T^CCGGA
5753 TCCGGA
BsrBI 1358 CCGCTC CCGCTC(-3/-3)
507 GAGCGG
BsrGI 421 TGTACA T^GTACA
1025 TGTACA
BstEII 1684 GGTAACC G^GT[ACGT]ACC
5838 GGTGACC
BstPI 1684 GGTAACC G^GT[ACGT]ACC
5838 GGTGACC
BstXI 2798 CCAAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5617 CCAGCACTCTGG
Eam1105I 329 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
348 GACTCCTGGTC
Eco47III 311 AGCGCT AGC^GCT
610 AGCGCT
EcoO65I 1684 GGTAACC G^GT[ACGT]ACC
5838 GGTGACC
EcoRI 1438 GAATTC G^AATTC
1666 GAATTC
HgaI 3 GCGTC GACGC(5/10)
817 GCGTC
HindIII 1269 AAGCTT A^AGCTT
2657 AAGCTT
MluNI 283 TGGCCA TGG^CCA
3883 TGGCCA
MroI 1012 TCCGGA T^CCGGA
5753 TCCGGA
MscI 283 TGGCCA TGG^CCA
3883 TGGCCA
NcoI 604 CCATGG C^CATGG
4709 CCATGG
PflFI 3815 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4926 GACCCTGTC
PmaCI 1239 CACGTG CAC^GTG
4399 CACGTG
PmlI 1239 CACGTG CAC^GTG
4399 CACGTG
SspBI 421 TGTACA T^GTACA
1025 TGTACA
SspI 3383 AATATT AAT^ATT
3453 AATATT
Tth111I 3815 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4926 GACCCTGTC
XbaI 5163 TCTAGA T^CTAGA
5282 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI