##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
AspEI AviII AvrII BbeI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI KasI MamI MfeI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SapI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SwaI TfiI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 1080 GGTACC G^GTACC
AflIII 28 ACGCGT A^C[GA][CT]GT
AocI 485 CCTGAGG CC^T[ACGT]AGG
Aor51HI 181 AGCGCT AGC^GCT
AseI 882 ATTAAT AT^TAAT
AsnI 882 ATTAAT AT^TAAT
Asp700I 1202 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1080 GGTACC G^GTACC
AspHI 668 GAGCAC G[AT]GC[AT]^C
AspI 316 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1090 GGATCC G^GATCC
BbrPI 740 CACGTG CAC^GTG
BbvCI 468 GCTGAGG CCTCAGC(-5/-2)
BglI 134 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 740 CACGTG [CT]AC^GT[GA]
BsgI 784 GTGCAG GTGCAG(16/14)
BsiEI 675 CGGCCG CG[GA][CT]^CG
BsiHKAI 668 GAGCAC G[AT]GC[AT]^C
BsmAI 216 GAGAC GTCTC(1/5)
BsmI 158 GAATGC GAATGC(1/-1)
BspMI 305 ACCTGC ACCTGC(4/8)
BsrBI 1216 CCGCTC CCGCTC(-3/-3)
BstAPI 79 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1274 GGTCACC G^GT[ACGT]ACC
BstPI 1274 GGTCACC G^GT[ACGT]ACC
Bsu36I 485 CCTGAGG CC^T[ACGT]AGG
DraIII 1039 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 675 CGGCCG C^GGCCG
EclXI 675 CGGCCG C^GGCCG
Eco47III 181 AGCGCT AGC^GCT
Eco52I 675 CGGCCG C^GGCCG
Eco81I 485 CCTGAGG CC^T[ACGT]AGG
EcoNI 223 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1274 GGTCACC G^GT[ACGT]ACC
EcoRV 20 GATATC GAT^ATC
HincII 867 GTCGAC GT[CT]^[GA]AC
HindII 867 GTCGAC GT[CT]^[GA]AC
KpnI 1080 GGTACC GGTAC^C
KspI 678 CCGCGG CCGC^GG
MluI 28 ACGCGT A^CGCGT
MspA1I 678 CCGCGG C[AC]G^C[GT]G
NcoI 351 CCATGG C^CATGG
PflFI 316 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 740 CACGTG CAC^GTG
PmlI 740 CACGTG CAC^GTG
PpuMI 1241 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 882 ATTAAT AT^TAAT
PstI 438 CTGCAG CTGCA^G
SacII 678 CCGCGG CCGC^GG
SalI 867 GTCGAC G^TCGAC
SbfI 437 CCTGCAGG CCTGCA^GG
SfiI 133 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 135 CCCGGG CCC^GGG
SmlI 1222 CTTGAG C^T[CT][GA]AG
Sse8387I 437 CCTGCAGG CCTGCA^GG
SspI 1363 AATATT AAT^ATT
StuI 482 AGGCCT AGG^CCT
Tsp509I 1033 AATT ^AATT
Tth111I 316 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 135 CCCGGG C^CCGGG
XmaI 135 CCCGGG C^CCGGG
XmnI 1202 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 751 GTAGAC GT^[AC][GT]AC
867 GTCGAC
AfaI 624 GTAC GT^AC
1081 GTAC
AvaI 135 CCCGGG C^[CT]CG[GA]G
1249 CTCGGG
BanI 984 GGCACC G^G[CT][GA]CC
1080 GGTACC
BsmFI 362 GTCCC GGGAC(10/14)
424 GTCCC
BsoBI 135 CCCGGG C^[CT]CG[GA]G
1249 CTCGGG
BstXI 228 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1139 CCAGCTTCCTGG
BstYI 414 AGATCC [GA]^GATC[CT]
1090 GGATCC
HinfI 422 GAGTC G^A[ACGT]TC
507 GACTC
MflI 414 AGATCC [GA]^GATC[CT]
1090 GGATCC
MseI 883 TTAA T^TAA
1357 TTAA
NheI 178 GCTAGC G^CTAGC
389 GCTAGC
PleI 507 GACTC GAGTC(4/5)
422 GAGTC
RsaI 624 GTAC GT^AC
1081 GTAC
SphI 609 GCATGC GCATG^C
1004 GCATGC
Tru9I 883 TTAA T^TAA
1357 TTAA
XhoII 414 AGATCC [GA]^GATC[CT]
1090 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AatII AccII AciI AcyI AluI
AlwI AlwNI ApaI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I