##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AgeI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BcgI BglII BlnI BsaBI BsiWI
Bsp1407I BspDI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI EcoRI EcoRV FspI HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI RsrII SacI SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3568 GTCTAC GT^[AC][GT]AC
AccIII 285 TCCGGA T^CCGGA
AclI 497 AACGTT AA^CGTT
AcsI 3965 AAATTT [GA]^AATT[CT]
AhdI 1292 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2284 GTGCAC G^TGCAC
ApaLI 2284 GTGCAC G^TGCAC
ApoI 3965 AAATTT [GA]^AATT[CT]
Asp700I 4091 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1292 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2484 GGATCC G^GATCC
BciVI 2234 GGATAC GTATCC(6/5)
BsaWI 285 TCCGGA [AT]^CCGG[AT]
BseAI 285 TCCGGA T^CCGGA
BspEI 285 TCCGGA T^CCGGA
BspHI 1344 TCATGA T^CATGA
BspLU11I 4032 ACATGT A^CATGT
BstEII 1480 GGTGACC G^GT[ACGT]ACC
BstPI 1480 GGTGACC G^GT[ACGT]ACC
DrdI 407 GACCCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 515 CGGCCG C^GGCCG
Eam1105I 1292 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 515 CGGCCG C^GGCCG
Eco52I 515 CGGCCG C^GGCCG
EcoO65I 1480 GGTGACC G^GT[ACGT]ACC
FseI 512 GGCCGGCC GGCCGG^CC
HindIII 2553 AAGCTT A^AGCTT
KspI 3880 CCGCGG CCGC^GG
MroI 285 TCCGGA T^CCGGA
NdeI 3913 CATATG CA^TATG
Psp1406I 497 AACGTT AA^CGTT
PvuI 1754 CGATCG CGAT^CG
RcaI 1344 TCATGA T^CATGA
SacII 3880 CCGCGG CCGC^GG
SapI 3197 GCTCTTC GCTCTTC(1/4)
SexAI 267 ACCTGGT A^CC[AT]GGT
SphI 3491 GCATGC GCATG^C
SspI 3920 AATATT AAT^ATT
SwaI 4275 ATTTAAAT ATTT^AAAT
XbaI 2330 TCTAGA T^CTAGA
XmnI 4091 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 2291 CGCG CG^CG
3881 CGCG
AflII 2045 CTTAAG C^TTAAG
2466 CTTAAG
AflIII 1846 ACACGT A^C[GA][CT]GT
4032 ACATGT
Aor51HI 354 AGCGCT AGC^GCT
2792 AGCGCT
AspI 1613 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3276 GACCCTGTC
BbrPI 44 CACGTG CAC^GTG
467 CACGTG
BbsI 1160 GAAGAC GAAGAC(2/6)
1289 GAAGAC
BclI 123 TGATCA T^GATCA
1707 TGATCA
BfrI 2045 CTTAAG C^TTAAG
2466 CTTAAG
BpuAI 1160 GAAGAC GAAGAC(2/6)
1289 GAAGAC
BsiEI 515 CGGCCG CG[GA][CT]^CG
1754 CGATCG
BsmBI 975 CGTCTC CGTCTC(1/5)
1525 CGTCTC
BsmI 480 GCATTC GAATGC(1/-1)
2269 GCATTC
BstAPI 244 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2059 GCACAGGCTGC
BstUI 2291 CGCG CG^CG
3881 CGCG
DraI 4261 TTTAAA TTT^AAA
4276 TTTAAA
Eco47III 354 AGCGCT AGC^GCT
2792 AGCGCT
EcoT22I 2252 ATGCAT ATGCA^T
4007 ATGCAT
FbaI 123 TGATCA T^GATCA
1707 TGATCA
HincII 1202 GTTGAC GT[CT]^[GA]AC
1628 GTCAAC
HindII 1202 GTTGAC GT[CT]^[GA]AC
1628 GTCAAC
MvnI 2291 CGCG CG^CG
3881 CGCG
NsiI 2252 ATGCAT ATGCA^T
4007 ATGCAT
PaeR7I 1130 CTCGAG C^TCGAG
1373 CTCGAG
PflFI 1613 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3276 GACCCTGTC
PmaCI 44 CACGTG CAC^GTG
467 CACGTG
PmlI 44 CACGTG CAC^GTG
467 CACGTG
PvuII 891 CAGCTG CAG^CTG
1993 CAGCTG
SbfI 541 CCTGCAGG CCTGCA^GG
1621 CCTGCAGG
SmaI 798 CCCGGG CCC^GGG
1065 CCCGGG
Sse8387I 541 CCTGCAGG CCTGCA^GG
1621 CCTGCAGG
Tth111I 1613 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3276 GACCCTGTC
XhoI 1130 CTCGAG C^TCGAG
1373 CTCGAG
XmaCI 798 CCCGGG C^CCGGG
1065 CCCGGG
XmaI 798 CCCGGG C^CCGGG
1065 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII