##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AccII AclI AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII BbeI BclI BsiEI
BsiWI BspDI BspHI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 181 TCCGGA T^CCGGA
AflII 3113 CTTAAG C^TTAAG
Alw44I 2716 GTGCAC G^TGCAC
Aor51HI 2393 AGCGCT AGC^GCT
ApaLI 2716 GTGCAC G^TGCAC
AvrII 1866 CCTAGG C^CTAGG
BcgI 3540 CGAGGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3651 GTATCC GTATCC(6/5)
BfrI 3113 CTTAAG C^TTAAG
BglII 2252 AGATCT A^GATCT
BlnI 1866 CCTAGG C^CTAGG
BsaI 1187 GAGACC GGTCTC(1/5)
BsaWI 181 TCCGGA [AT]^CCGG[AT]
BseAI 181 TCCGGA T^CCGGA
BsmI 1343 GCATTC GAATGC(1/-1)
Bsp1407I 1159 TGTACA T^GTACA
BspEI 181 TCCGGA T^CCGGA
BsrBI 3628 GAGCGG CCGCTC(-3/-3)
BsrGI 1159 TGTACA T^GTACA
Eco47III 2393 AGCGCT AGC^GCT
HaeII 2393 AGCGCT [GA]GCGC^[CT]
HgaI 3602 GCGTC GACGC(5/10)
HincII 2756 GTCAAC GT[CT]^[GA]AC
HindII 2756 GTCAAC GT[CT]^[GA]AC
HindIII 227 AAGCTT A^AGCTT
MroI 181 TCCGGA T^CCGGA
PshAI 1038 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 263 GAAGAGC GCTCTTC(1/4)
SexAI 175 ACCTGGT A^CC[AT]GGT
SmaI 3001 CCCGGG CCC^GGG
SphI 379 GCATGC GCATG^C
SspBI 1159 TGTACA T^GTACA
SspI 4162 AATATT AAT^ATT
XmaCI 3001 CCCGGG C^CCGGG
XmaI 3001 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 303 GACGTC GACGT^C
3734 GACGTC
AcsI 72 AAATTT [GA]^AATT[CT]
444 AAATTC
AcyI 303 GACGTC G[GA]^CG[CT]C
3734 GACGTC
AflIII 1156 ACATGT A^C[GA][CT]GT
1312 ACATGT
AocI 2285 CCTGAGG CC^T[ACGT]AGG
2335 CCTCAGG
ApoI 72 AAATTT [GA]^AATT[CT]
444 AAATTC
AspI 480 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3398 GACAAGGTC
AvaI 3001 CCCGGG C^[CT]CG[GA]G
3538 CCCGAG
BamHI 2116 GGATCC G^GATCC
2277 GGATCC
BsaBI 2247 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3889 GATCATCATC
BsaHI 303 GACGTC G[GA]^CG[CT]C
3734 GACGTC
BsoBI 3001 CCCGGG C^[CT]CG[GA]G
3538 CCCGAG
BspLU11I 1156 ACATGT A^CATGT
1312 ACATGT
BsrDI 4060 CATTGC GCAATG(2/0)
1638 GCAATG
Bsu36I 2285 CCTGAGG CC^T[ACGT]AGG
2335 CCTCAGG
DraI 87 TTTAAA TTT^AAA
4562 TTTAAA
DraIII 255 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1668 CACATAGTG
DrdI 114 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3911 GACGGCATGGTC
Eco81I 2285 CCTGAGG CC^T[ACGT]AGG
2335 CCTCAGG
EcoT22I 843 ATGCAT ATGCA^T
3186 ATGCAT
Hin1I 303 GACGTC G[GA]^CG[CT]C
3734 GACGTC
MamI 2247 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3889 GATCATCATC
NsiI 843 ATGCAT ATGCA^T
3186 ATGCAT
PflFI 480 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3398 GACAAGGTC
PflMI 2354 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3816 CCACAGCCTGG
StuI 202 AGGCCT AGG^CCT
3561 AGGCCT
Tth111I 480 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3398 GACAAGGTC
Van91I 2354 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3816 CCACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI Asp700I AspHI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI