##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII ApoI AscI AseI
AsnI AviII AvrII BamHI BbrPI
BcgI BciVI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoRI FseI FspI
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 874 CGCG CG^CG
AhdI 2812 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2858 GTGCAC G^TGCAC
Aor51HI 3136 AGCGCT AGC^GCT
ApaI 4268 GGGCCC GGGCC^C
ApaLI 2858 GTGCAC G^TGCAC
Asp700I 3843 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2812 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4168 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 4052 TGATCA T^GATCA
BglI 168 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 1259 CGTCTC CGTCTC(1/5)
BsrBI 570 GAGCGG CCGCTC(-3/-3)
BstAPI 2352 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 874 CGCG CG^CG
DraI 1013 TTTAAA TTT^AAA
Eam1105I 2812 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3136 AGCGCT AGC^GCT
EcoRV 101 GATATC GAT^ATC
FbaI 4052 TGATCA T^GATCA
HgaI 3804 GCGTC GACGC(5/10)
MaeII 1258 ACGT A^CGT
MvnI 874 CGCG CG^CG
NcoI 3668 CCATGG C^CATGG
PflFI 4168 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 2646 AGTACT AGT^ACT
SexAI 4169 ACCAGGT A^CC[AT]GGT
SphI 3407 GCATGC GCATG^C
Tth111I 4168 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3843 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1243 GGTACC G^GTACC
2244 GGTACC
AgeI 665 ACCGGT A^CCGGT
1759 ACCGGT
Asp718I 1243 GGTACC G^GTACC
2244 GGTACC
BbeI 1986 GGCGCC GGCGC^C
3947 GGCGCC
BspHI 1365 TCATGA T^CATGA
4049 TCATGA
BspMI 3017 ACCTGC ACCTGC(4/8)
3345 GCAGGT
BsrDI 1810 CATTGC GCAATG(2/0)
335 GCAATG
BstEII 692 GGTGACC G^GT[ACGT]ACC
1865 GGTGACC
BstPI 692 GGTGACC G^GT[ACGT]ACC
1865 GGTGACC
DsaI 1779 CCGTGG C^C[GA][CT]GG
3668 CCATGG
EcoO65I 692 GGTGACC G^GT[ACGT]ACC
1865 GGTGACC
EcoT22I 328 ATGCAT ATGCA^T
1455 ATGCAT
KasI 1986 GGCGCC G^GCGCC
3947 GGCGCC
KpnI 1243 GGTACC GGTAC^C
2244 GGTACC
NarI 1986 GGCGCC GG^CGCC
3947 GGCGCC
NheI 607 GCTAGC G^CTAGC
1292 GCTAGC
NsiI 328 ATGCAT ATGCA^T
1455 ATGCAT
PinAI 665 ACCGGT A^CCGGT
1759 ACCGGT
RcaI 1365 TCATGA T^CATGA
4049 TCATGA
SfoI 1986 GGCGCC GGC^GCC
3947 GGCGCC
SgrAI 664 CACCGGTG C[GA]^CCGG[CT]G
1758 CACCGGTG
XcmI 902 CCATAGTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3134 CCAGCGCTTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII XmaCI
XmaI