##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbsI BciVI BclI BfrI BglII
BpuAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfuI SgrAI SpeI SphI
SspBI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 809 GTAGAC GT^[AC][GT]AC
AflIII 3486 ACACGT A^C[GA][CT]GT
Alw44I 2876 GTGCAC G^TGCAC
ApaLI 2876 GTGCAC G^TGCAC
AvrII 3374 CCTAGG C^CTAGG
BbeI 1251 GGCGCC GGCGC^C
BbvCI 2660 CCTCAGC CCTCAGC(-5/-2)
BcgI 2822 GCAGGGTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3374 CCTAGG C^CTAGG
BsiEI 3833 CGGCCG CG[GA][CT]^CG
BsmBI 2199 GAGACG CGTCTC(1/5)
BsrFI 3918 GCCGGC [GA]^CCGG[CT]
BssSI 1990 CTCGTG CACGAG(-5/-1)
Cfr10I 3918 GCCGGC [GA]^CCGG[CT]
DraI 3059 TTTAAA TTT^AAA
EagI 3833 CGGCCG C^GGCCG
EclXI 3833 CGGCCG C^GGCCG
Eco52I 3833 CGGCCG C^GGCCG
KasI 1251 GGCGCC G^GCGCC
NaeI 3918 GCCGGC GCC^GGC
NarI 1251 GGCGCC GG^CGCC
NgoMIV 3918 GCCGGC G^CCGGC
NspI 2324 GCATGT [GA]CATG^[CT]
SbfI 4007 CCTGCAGG CCTGCA^GG
SexAI 4137 ACCTGGT A^CC[AT]GGT
SfiI 257 GGCCGCTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1251 GGCGCC GGC^GCC
SnaBI 3349 TACGTA TAC^GTA
Sse8387I 4007 CCTGCAGG CCTGCA^GG
StuI 2422 AGGCCT AGG^CCT
TaqI 3715 TCGA T^CGA
TthHB8I 3715 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 912 AAATTC [GA]^AATT[CT]
3062 AAATTT
AcyI 156 GACGCC G[GA]^CG[CT]C
1251 GGCGCC
AlwI 2575 GGATC GGATC(4/5)
4251 GGATC
ApoI 912 AAATTC [GA]^AATT[CT]
3062 AAATTT
AspI 527 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1087 GACTCTGTC
AviII 52 TGCGCA TGC^GCA
249 TGCGCA
BbrPI 2064 CACGTG CAC^GTG
4115 CACGTG
BsaHI 156 GACGCC G[GA]^CG[CT]C
1251 GGCGCC
BsmI 3480 GAATGC GAATGC(1/-1)
1286 GCATTC
BsrDI 1416 CATTGC GCAATG(2/0)
2511 CATTGC
BstEII 221 GGTGACC G^GT[ACGT]ACC
1116 GGTGACC
BstPI 221 GGTGACC G^GT[ACGT]ACC
1116 GGTGACC
BstYI 2575 GGATCT [GA]^GATC[CT]
4251 GGATCT
DpnII 2576 GATC ^GATC
4252 GATC
DraIII 1171 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3297 CACGATGTG
EcoO65I 221 GGTGACC G^GT[ACGT]ACC
1116 GGTGACC
FspI 52 TGCGCA TGC^GCA
249 TGCGCA
Hin1I 156 GACGCC G[GA]^CG[CT]C
1251 GGCGCC
HindIII 1144 AAGCTT A^AGCTT
1687 AAGCTT
MboI 2576 GATC ^GATC
4252 GATC
MflI 2575 GGATCT [GA]^GATC[CT]
4251 GGATCT
MseI 3034 TTAA T^TAA
3060 TTAA
MslI 1886 CACGCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3487 CACGTTGGTG
NcoI 1557 CCATGG C^CATGG
3791 CCATGG
NdeII 2576 GATC ^GATC
4252 GATC
PflFI 527 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1087 GACTCTGTC
PmaCI 2064 CACGTG CAC^GTG
4115 CACGTG
PmlI 2064 CACGTG CAC^GTG
4115 CACGTG
PshAI 445 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1653 GACAGATGTC
SacI 2160 GAGCTC GAGCT^C
3711 GAGCTC
SapI 1981 GAAGAGC GCTCTTC(1/4)
1297 GCTCTTC
Sau3AI 2576 GATC ^GATC
4252 GATC
Tru9I 3034 TTAA T^TAA
3060 TTAA
Tth111I 527 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1087 GACTCTGTC
XhoII 2575 GGATCT [GA]^GATC[CT]
4251 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XcmI XmaCI XmaI