##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AcsI AflII AhdI
ApoI AscI AseI AsnI Asp718I
AspEI BamHI BciVI BfrI BseAI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI Bst1107I BstBI BstXI BstZ17I
ClaI Eam1105I EcoRI EcoRV EcoT22I
HindIII HpaI KpnI MluI MroI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI PvuI SacI ScaI SfuI
SgrAI SpeI SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 299 GACGTC GACGT^C
AclI 3894 AACGTT AA^CGTT
Alw44I 1470 GTGCAC G^TGCAC
Aor51HI 1432 AGCGCT AGC^GCT
ApaLI 1470 GTGCAC G^TGCAC
Asp700I 2857 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2330 CCTAGG C^CTAGG
BbeI 3255 GGCGCC GGCGC^C
BbvCI 2777 GCTGAGG CCTCAGC(-5/-2)
BclI 2149 TGATCA T^GATCA
BglII 2440 AGATCT A^GATCT
BlnI 2330 CCTAGG C^CTAGG
BsiWI 61 CGTACG C^GTACG
BsmBI 2065 GAGACG CGTCTC(1/5)
BspHI 2771 TCATGA T^CATGA
BssHII 3068 GCGCGC G^CGCGC
BssSI 2734 CTCGTG CACGAG(-5/-1)
BstEII 2640 GGTCACC G^GT[ACGT]ACC
BstPI 2640 GGTCACC G^GT[ACGT]ACC
BstYI 2440 AGATCT [GA]^GATC[CT]
CpoI 234 CGGTCCG CG^G[AT]CCG
DraI 3478 TTTAAA TTT^AAA
Eco47III 1432 AGCGCT AGC^GCT
EcoO65I 2640 GGTCACC G^GT[ACGT]ACC
FbaI 2149 TGATCA T^GATCA
FseI 1256 GGCCGGCC GGCCGG^CC
KasI 3255 GGCGCC G^GCGCC
MfeI 4069 CAATTG C^AATTG
MflI 2440 AGATCT [GA]^GATC[CT]
MunI 4069 CAATTG C^AATTG
NarI 3255 GGCGCC GG^CGCC
Psp1406I 3894 AACGTT AA^CGTT
RcaI 2771 TCATGA T^CATGA
RsrII 234 CGGTCCG CG^G[AT]CCG
SalI 1407 GTCGAC G^TCGAC
SapI 2561 GAAGAGC GCTCTTC(1/4)
SexAI 394 ACCTGGT A^CC[AT]GGT
SfiI 1260 GGCCTGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3255 GGCGCC GGC^GCC
StuI 3559 AGGCCT AGG^CCT
TfiI 3598 GAATC G^A[AT]TC
Tsp509I 4070 AATT ^AATT
XhoII 2440 AGATCT [GA]^GATC[CT]
XmnI 2857 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1350 GTAGAC GT^[AC][GT]AC
1407 GTCGAC
AgeI 210 ACCGGT A^CCGGT
232 ACCGGT
AocI 3241 CCTCAGG CC^T[ACGT]AGG
3963 CCTGAGG
AvaI 17 CCCGGG C^[CT]CG[GA]G
3286 CCCGGG
AviII 1621 TGCGCA TGC^GCA
2015 TGCGCA
BbrPI 578 CACGTG CAC^GTG
3181 CACGTG
BbsI 1184 GAAGAC GAAGAC(2/6)
36 GTCTTC
BglI 1261 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2292 GCCCATGGGGC
BpuAI 1184 GAAGAC GAAGAC(2/6)
36 GTCTTC
BsaI 1211 GGTCTC GGTCTC(1/5)
3586 GGTCTC
BsiEI 1255 CGGCCG CG[GA][CT]^CG
2138 CGGCCG
BsoBI 17 CCCGGG C^[CT]CG[GA]G
3286 CCCGGG
BsrBI 2626 CCGCTC CCGCTC(-3/-3)
3935 CCGCTC
BsrDI 512 CATTGC GCAATG(2/0)
3684 CATTGC
Bsu36I 3241 CCTCAGG CC^T[ACGT]AGG
3963 CCTGAGG
DraIII 1518 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3876 CACATGGTG
DrdI 522 GACGGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2258 GACACACCTGTC
EagI 1255 CGGCCG C^GGCCG
2138 CGGCCG
EclXI 1255 CGGCCG C^GGCCG
2138 CGGCCG
Eco52I 1255 CGGCCG C^GGCCG
2138 CGGCCG
Eco81I 3241 CCTCAGG CC^T[ACGT]AGG
3963 CCTGAGG
FspI 1621 TGCGCA TGC^GCA
2015 TGCGCA
HincII 1407 GTCGAC GT[CT]^[GA]AC
4034 GTCAAC
HindII 1407 GTCGAC GT[CT]^[GA]AC
4034 GTCAAC
KspI 878 CCGCGG CCGC^GG
1801 CCGCGG
PinAI 210 ACCGGT A^CCGGT
232 ACCGGT
PmaCI 578 CACGTG CAC^GTG
3181 CACGTG
PmlI 578 CACGTG CAC^GTG
3181 CACGTG
SacII 878 CCGCGG CCGC^GG
1801 CCGCGG
SbfI 383 CCTGCAGG CCTGCA^GG
539 CCTGCAGG
SmaI 17 CCCGGG CCC^GGG
3286 CCCGGG
SmlI 246 CTCAAG C^T[CT][GA]AG
2689 CTCAAG
SnaBI 59 TACGTA TAC^GTA
721 TACGTA
SphI 406 GCATGC GCATG^C
1483 GCATGC
Sse8387I 383 CCTGCAGG CCTGCA^GG
539 CCTGCAGG
XmaCI 17 CCCGGG C^CCGGG
3286 CCCGGG
XmaI 17 CCCGGG C^CCGGG
3286 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AspI AvaII BalI BanI BanII
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI