##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII Alw44I Aor51HI ApaLI AscI
AspI AviII BamHI BbeI BclI
BfrI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
FbaI FseI FspI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SfoI
SfuI SgrAI SnaBI SspBI SspI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 4445 GGCGTC G[GA]^CG[CT]C
AgeI 2036 ACCGGT A^CCGGT
ApaI 4571 GGGCCC GGGCC^C
AseI 2857 ATTAAT AT^TAAT
AsnI 2857 ATTAAT AT^TAAT
BbrPI 3796 CACGTG CAC^GTG
BcgI 2507 CGACTGTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 451 AGATCT A^GATCT
BlpI 4227 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4227 GCTCAGC GC^T[ACGT]AGC
BsaAI 3796 CACGTG [CT]AC^GT[GA]
BsaHI 4445 GGCGTC G[GA]^CG[CT]C
BsaWI 2036 ACCGGT [AT]^CCGG[AT]
BspHI 3710 TCATGA T^CATGA
BsrBI 4702 GAGCGG CCGCTC(-3/-3)
BsrFI 2036 ACCGGT [GA]^CCGG[CT]
BssSI 1503 CTCGTG CACGAG(-5/-1)
BstAPI 3314 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4227 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2036 ACCGGT [GA]^CCGG[CT]
Hin1I 4445 GGCGTC G[GA]^CG[CT]C
HincII 2171 GTTAAC GT[CT]^[GA]AC
HindII 2171 GTTAAC GT[CT]^[GA]AC
HindIII 3005 AAGCTT A^AGCTT
HpaI 2171 GTTAAC GTT^AAC
PinAI 2036 ACCGGT A^CCGGT
PmaCI 3796 CACGTG CAC^GTG
PmlI 3796 CACGTG CAC^GTG
PshBI 2857 ATTAAT AT^TAAT
RcaI 3710 TCATGA T^CATGA
SacI 4339 GAGCTC GAGCT^C
SbfI 1993 CCTGCAGG CCTGCA^GG
ScaI 2592 AGTACT AGT^ACT
SexAI 1097 ACCAGGT A^CC[AT]GGT
SfiI 3184 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2465 ACTAGT A^CTAGT
SphI 4546 GCATGC GCATG^C
Sse8387I 1993 CCTGCAGG CCTGCA^GG
SwaI 2832 ATTTAAAT ATTT^AAAT
TaqI 726 TCGA T^CGA
TthHB8I 726 TCGA T^CGA
XbaI 4492 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 1078 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3738 GACCTATAGTC
Asp700I 2581 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4136 GAAGAGGTTC
AspEI 1078 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3738 GACCTATAGTC
AvaI 1590 CCCGGG C^[CT]CG[GA]G
2344 CCCGGG
BciVI 2487 GGATAC GTATCC(6/5)
2180 GTATCC
BglI 874 GCCCCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3185 GCCGAGCTGGC
BsmBI 4119 CGTCTC CGTCTC(1/5)
1076 GAGACG
BsoBI 1590 CCCGGG C^[CT]CG[GA]G
2344 CCCGGG
BspLU11I 626 ACATGT A^CATGT
1082 ACATGT
BstYI 451 AGATCT [GA]^GATC[CT]
3334 AGATCC
DraI 2833 TTTAAA TTT^AAA
2953 TTTAAA
Eam1105I 1078 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3738 GACCTATAGTC
EarI 332 GAAGAG CTCTTC(1/4)
4136 GAAGAG
EcoT22I 441 ATGCAT ATGCA^T
2532 ATGCAT
HaeII 1976 GGCGCT [GA]GCGC^[CT]
4224 GGCGCT
Ksp632I 332 GAAGAG CTCTTC(1/4)
4136 GAAGAG
MflI 451 AGATCT [GA]^GATC[CT]
3334 AGATCC
NsiI 441 ATGCAT ATGCA^T
2532 ATGCAT
PshAI 503 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2603 GACCCATGTC
SmaI 1590 CCCGGG CCC^GGG
2344 CCCGGG
XhoII 451 AGATCT [GA]^GATC[CT]
3334 AGATCC
XmaCI 1590 CCCGGG C^CCGGG
2344 CCCGGG
XmaI 1590 CCCGGG C^CCGGG
2344 CCCGGG
XmnI 2581 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4136 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp718I AspHI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KpnI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI