##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AviII BcgI
BclI BfrI BglII BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2037 GGTACC G^GTACC
AccII 1479 CGCG CG^CG
AcyI 158 GGCGCC G[GA]^CG[CT]C
Asp718I 2037 GGTACC G^GTACC
AspI 1369 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3895 GGATCC G^GATCC
BbeI 158 GGCGCC GGCGC^C
BbrPI 2029 CACGTG CAC^GTG
BbsI 80 GAAGAC GAAGAC(2/6)
BciVI 2222 GTATCC GTATCC(6/5)
BpuAI 80 GAAGAC GAAGAC(2/6)
BsaAI 2029 CACGTG [CT]AC^GT[GA]
BsaBI 2851 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 158 GGCGCC G[GA]^CG[CT]C
BsmBI 3020 GAGACG CGTCTC(1/5)
BsmI 2024 GAATGC GAATGC(1/-1)
BsrBI 4187 CCGCTC CCGCTC(-3/-3)
BssHII 1478 GCGCGC G^CGCGC
BssSI 822 CACGAG CACGAG(-5/-1)
BstUI 1479 CGCG CG^CG
DraIII 7 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
HgaI 502 GCGTC GACGC(5/10)
Hin1I 158 GGCGCC G[GA]^CG[CT]C
KasI 158 GGCGCC G^GCGCC
KpnI 2037 GGTACC GGTAC^C
MamI 2851 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1479 CGCG CG^CG
NaeI 114 GCCGGC GCC^GGC
NarI 158 GGCGCC GG^CGCC
NgoMIV 114 GCCGGC G^CCGGC
NspI 1295 GCATGT [GA]CATG^[CT]
PaeR7I 3521 CTCGAG C^TCGAG
PflFI 1369 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1744 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2029 CACGTG CAC^GTG
PmlI 2029 CACGTG CAC^GTG
PshAI 3578 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 253 GAGCTC GAGCT^C
SfiI 2751 GGCCTGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 158 GGCGCC GGC^GCC
SmaI 2990 CCCGGG CCC^GGG
Tth111I 1369 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1744 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3521 CTCGAG C^TCGAG
XmaCI 2990 CCCGGG C^CCGGG
XmaI 2990 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvrII 779 CCTAGG C^CTAGG
807 CCTAGG
BlnI 779 CCTAGG C^CTAGG
807 CCTAGG
BlpI 1856 GCTTAGC GC^T[ACGT]AGC
3724 GCTGAGC
Bpu1102I 1856 GCTTAGC GC^T[ACGT]AGC
3724 GCTGAGC
BsrDI 2453 CATTGC GCAATG(2/0)
387 GCAATG
BsrFI 114 GCCGGC [GA]^CCGG[CT]
1809 GCCGGT
BstAPI 464 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1722 GCATCCCATGC
CelII 1856 GCTTAGC GC^T[ACGT]AGC
3724 GCTGAGC
Cfr10I 114 GCCGGC [GA]^CCGG[CT]
1809 GCCGGT
HaeII 158 GGCGCC [GA]GCGC^[CT]
3728 AGCGCC
TaqI 3099 TCGA T^CGA
3522 TCGA
TthHB8I 3099 TCGA T^CGA
3522 TCGA
XcmI 1163 CCATGAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3624 CCAGCTGGGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XhoII