##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BcgI BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 392 GACGTC GACGT^C
AccI 2541 GTAGAC GT^[AC][GT]AC
AcsI 1914 AAATTC [GA]^AATT[CT]
AflIII 2039 ACATGT A^C[GA][CT]GT
AgeI 4016 ACCGGT A^CCGGT
Alw44I 3034 GTGCAC G^TGCAC
Aor51HI 91 AGCGCT AGC^GCT
ApaLI 3034 GTGCAC G^TGCAC
ApoI 1914 AAATTC [GA]^AATT[CT]
AviII 489 TGCGCA TGC^GCA
BamHI 2579 GGATCC G^GATCC
BbrPI 1638 CACGTG CAC^GTG
BsaAI 1638 CACGTG [CT]AC^GT[GA]
BsaWI 4016 ACCGGT [AT]^CCGG[AT]
BsiEI 3666 CGGCCG CG[GA][CT]^CG
BsmI 1703 GCATTC GAATGC(1/-1)
Bsp1407I 1889 TGTACA T^GTACA
BspHI 3693 TCATGA T^CATGA
BspLU11I 2039 ACATGT A^CATGT
BsrDI 2389 CATTGC GCAATG(2/0)
BsrFI 4016 ACCGGT [GA]^CCGG[CT]
BsrGI 1889 TGTACA T^GTACA
BstEII 3530 GGTCACC G^GT[ACGT]ACC
BstPI 3530 GGTCACC G^GT[ACGT]ACC
Cfr10I 4016 ACCGGT [GA]^CCGG[CT]
DraIII 2698 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3666 CGGCCG C^GGCCG
EclXI 3666 CGGCCG C^GGCCG
Eco47III 91 AGCGCT AGC^GCT
Eco52I 3666 CGGCCG C^GGCCG
EcoNI 2098 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3530 GGTCACC G^GT[ACGT]ACC
FspI 489 TGCGCA TGC^GCA
HgaI 1680 GACGC GACGC(5/10)
MseI 1922 TTAA T^TAA
NcoI 3375 CCATGG C^CATGG
PinAI 4016 ACCGGT A^CCGGT
PmaCI 1638 CACGTG CAC^GTG
PmlI 1638 CACGTG CAC^GTG
PshAI 1680 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3693 TCATGA T^CATGA
SacI 3252 GAGCTC GAGCT^C
SspBI 1889 TGTACA T^GTACA
Tru9I 1922 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
Asp700I 1895 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2006 GAACATTTTC
BstAPI 3036 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3426 GCATGGGATGC
HincII 173 GTTGAC GT[CT]^[GA]AC
347 GTTGAC
HindII 173 GTTGAC GT[CT]^[GA]AC
347 GTTGAC
NheI 1213 GCTAGC G^CTAGC
1723 GCTAGC
NspI 2039 ACATGT [GA]CATG^[CT]
2299 ACATGC
PaeR7I 359 CTCGAG C^TCGAG
4055 CTCGAG
PstI 1533 CTGCAG CTGCA^G
1562 CTGCAG
SexAI 405 ACCAGGT A^CC[AT]GGT
756 ACCAGGT
XcmI 1127 CCAGGAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3792 CCAGCTTAGAGATGG
XhoI 359 CTCGAG C^TCGAG
4055 CTCGAG
XmnI 1895 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2006 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI