##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
AscI AseI AsnI AspEI AviII
BglII BsaBI BsiEI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KspI
MamI MluI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SpeI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3658 GGTACC G^GTACC
AccIII 4214 TCCGGA T^CCGGA
Alw44I 2932 GTGCAC G^TGCAC
Aor51HI 2714 AGCGCT AGC^GCT
ApaLI 2932 GTGCAC G^TGCAC
Asp718I 3658 GGTACC G^GTACC
AspI 1660 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1290 CCTAGG C^CTAGG
BbrPI 1990 CACGTG CAC^GTG
BcgI 559 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 757 GGATAC GTATCC(6/5)
BclI 319 TGATCA T^GATCA
BlnI 1290 CCTAGG C^CTAGG
BseAI 4214 TCCGGA T^CCGGA
BsmBI 2836 GAGACG CGTCTC(1/5)
BsmI 1121 GCATTC GAATGC(1/-1)
Bsp1407I 1220 TGTACA T^GTACA
BspEI 4214 TCCGGA T^CCGGA
BspLU11I 1692 ACATGT A^CATGT
BsrDI 2381 GCAATG GCAATG(2/0)
BsrFI 3290 GCCGGC [GA]^CCGG[CT]
BsrGI 1220 TGTACA T^GTACA
Cfr10I 3290 GCCGGC [GA]^CCGG[CT]
DraIII 861 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2714 AGCGCT AGC^GCT
FbaI 319 TGATCA T^GATCA
KpnI 3658 GGTACC GGTAC^C
MfeI 820 CAATTG C^AATTG
MroI 4214 TCCGGA T^CCGGA
MunI 820 CAATTG C^AATTG
NaeI 3290 GCCGGC GCC^GGC
NcoI 1616 CCATGG C^CATGG
NgoMIV 3290 GCCGGC G^CCGGC
PflFI 1660 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1990 CACGTG CAC^GTG
PmlI 1990 CACGTG CAC^GTG
SnaBI 1722 TACGTA TAC^GTA
SphI 2553 GCATGC GCATG^C
SspBI 1220 TGTACA T^GTACA
Tth111I 1660 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1900 CGCG CG^CG
4317 CGCG
AcsI 3357 GAATTT [GA]^AATT[CT]
4489 AAATTC
AflII 3249 CTTAAG C^TTAAG
4151 CTTAAG
ApoI 3357 GAATTT [GA]^AATT[CT]
4489 AAATTC
Asp700I 2712 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4485 GAATAAATTC
BbeI 61 GGCGCC GGCGC^C
2808 GGCGCC
BbsI 4128 GTCTTC GAAGAC(2/6)
4370 GTCTTC
BfrI 3249 CTTAAG C^TTAAG
4151 CTTAAG
BpuAI 4128 GTCTTC GAAGAC(2/6)
4370 GTCTTC
BsaAI 1722 TACGTA [CT]AC^GT[GA]
1990 CACGTG
BsaWI 1717 TCCGGT [AT]^CCGG[AT]
4214 TCCGGA
BsgI 1434 GTGCAG GTGCAG(16/14)
3439 GTGCAG
BstUI 1900 CGCG CG^CG
4317 CGCG
DraI 1543 TTTAAA TTT^AAA
2590 TTTAAA
KasI 61 GGCGCC G^GCGCC
2808 GGCGCC
MslI 1988 CACACGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2027 CACCCCCATG
MvnI 1900 CGCG CG^CG
4317 CGCG
NarI 61 GGCGCC GG^CGCC
2808 GGCGCC
SacI 737 GAGCTC GAGCT^C
2635 GAGCTC
SapI 1767 GCTCTTC GCTCTTC(1/4)
2353 GCTCTTC
SfoI 61 GGCGCC GGC^GCC
2808 GGCGCC
SmaI 1739 CCCGGG CCC^GGG
3067 CCCGGG
XmaCI 1739 CCCGGG C^CCGGG
3067 CCCGGG
XmaI 1739 CCCGGG C^CCGGG
3067 CCCGGG
XmnI 2712 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4485 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII