##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I BbeI BciVI
BfrI BsiWI Bsp1407I BspDI BspHI
BsrDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
FseI HaeII HpaI KasI KpnI
MfeI MunI NarI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SapI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 9 TCCGGA T^CCGGA
AcyI 4410 GGCGTC G[GA]^CG[CT]C
AhdI 1110 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1110 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 587 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4089 TGCGCA TGC^GCA
AvrII 3602 CCTAGG C^CTAGG
BamHI 3352 GGATCC G^GATCC
BclI 3162 TGATCA T^GATCA
BglII 1554 AGATCT A^GATCT
BlnI 3602 CCTAGG C^CTAGG
BsaBI 1398 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4410 GGCGTC G[GA]^CG[CT]C
BsaI 2343 GGTCTC GGTCTC(1/5)
BsaWI 9 TCCGGA [AT]^CCGG[AT]
BseAI 9 TCCGGA T^CCGGA
BsmBI 4157 GAGACG CGTCTC(1/5)
BsmI 1304 GCATTC GAATGC(1/-1)
BspEI 9 TCCGGA T^CCGGA
BspLU11I 3622 ACATGT A^CATGT
BssHII 4181 GCGCGC G^CGCGC
BstEII 3388 GGTCACC G^GT[ACGT]ACC
BstPI 3388 GGTCACC G^GT[ACGT]ACC
EagI 3866 CGGCCG C^GGCCG
Eam1105I 1110 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3866 CGGCCG C^GGCCG
Eco52I 3866 CGGCCG C^GGCCG
EcoO65I 3388 GGTCACC G^GT[ACGT]ACC
EcoRI 448 GAATTC G^AATTC
EcoRV 3793 GATATC GAT^ATC
EcoT22I 3064 ATGCAT ATGCA^T
FbaI 3162 TGATCA T^GATCA
FspI 4089 TGCGCA TGC^GCA
Hin1I 4410 GGCGTC G[GA]^CG[CT]C
HincII 1511 GTCGAC GT[CT]^[GA]AC
HindII 1511 GTCGAC GT[CT]^[GA]AC
HindIII 55 AAGCTT A^AGCTT
KspI 3181 CCGCGG CCGC^GG
MamI 1398 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4 ACGCGT A^CGCGT
MroI 9 TCCGGA T^CCGGA
NaeI 4377 GCCGGC GCC^GGC
NgoMIV 4377 GCCGGC G^CCGGC
NheI 1961 GCTAGC G^CTAGC
NsiI 3064 ATGCAT ATGCA^T
PflFI 587 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 948 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3181 CCGCGG CCGC^GG
SalI 1511 GTCGAC G^TCGAC
ScaI 1896 AGTACT AGT^ACT
SexAI 4083 ACCAGGT A^CC[AT]GGT
SmlI 1298 CTCAAG C^T[CT][GA]AG
TfiI 1403 GAATC G^A[AT]TC
Tth111I 587 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 1039 GTAGAC GT^[AC][GT]AC
1511 GTCGAC
AcsI 448 GAATTC [GA]^AATT[CT]
3738 GAATTT
ApoI 448 GAATTC [GA]^AATT[CT]
3738 GAATTT
BbsI 1326 GAAGAC GAAGAC(2/6)
908 GTCTTC
BbvCI 1226 GCTGAGG CCTCAGC(-5/-2)
4381 GCTGAGG
BcgI 3956 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4095 GCAGCCAGTTCG
BpuAI 1326 GAAGAC GAAGAC(2/6)
908 GTCTTC
BsiEI 369 CGACCG CG[GA][CT]^CG
3866 CGGCCG
BssSI 842 CACGAG CACGAG(-5/-1)
2786 CTCGTG
BstAPI 787 GCACTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2397 GCAGCGACTGC
EcoNI 1407 CCTAATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2770 CCTGCATCAGG
NspI 3066 GCATGT [GA]CATG^[CT]
3622 ACATGT
SacI 436 GAGCTC GAGCT^C
3280 GAGCTC
SfiI 185 GGCCGGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4233 GGCCCGGGAGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmaI Sse8387I StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI