##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AscI
AseI AsnI Asp718I AspI BbrPI
BglII BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
HpaI KpnI MaeII MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2718 GTCGAC GT^[AC][GT]AC
AcsI 2121 AAATTC [GA]^AATT[CT]
AflII 4185 CTTAAG C^TTAAG
AflIII 1655 ACATGT A^C[GA][CT]GT
AgeI 3551 ACCGGT A^CCGGT
AhdI 3540 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3301 AGCGCT AGC^GCT
ApoI 2121 AAATTC [GA]^AATT[CT]
AspEI 3540 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2791 TGCGCA TGC^GCA
AvrII 2971 CCTAGG C^CTAGG
BamHI 776 GGATCC G^GATCC
BbvCI 3012 CCTCAGC CCTCAGC(-5/-2)
BciVI 863 GGATAC GTATCC(6/5)
BclI 3 TGATCA T^GATCA
BfrI 4185 CTTAAG C^TTAAG
BlnI 2971 CCTAGG C^CTAGG
BsiEI 3553 CGGTCG CG[GA][CT]^CG
BspLU11I 1655 ACATGT A^CATGT
BsrFI 3551 ACCGGT [GA]^CCGG[CT]
BstAPI 1278 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 237 GGTGACC G^GT[ACGT]ACC
BstPI 237 GGTGACC G^GT[ACGT]ACC
Cfr10I 3551 ACCGGT [GA]^CCGG[CT]
Eam1105I 3540 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3301 AGCGCT AGC^GCT
EcoO65I 237 GGTGACC G^GT[ACGT]ACC
FbaI 3 TGATCA T^GATCA
FspI 2791 TGCGCA TGC^GCA
HindIII 2630 AAGCTT A^AGCTT
KspI 1973 CCGCGG CCGC^GG
NheI 2428 GCTAGC G^CTAGC
PinAI 3551 ACCGGT A^CCGGT
PshAI 4125 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1973 CCGCGG CCGC^GG
SalI 2718 GTCGAC G^TCGAC
SapI 1105 GCTCTTC GCTCTTC(1/4)
SfiI 972 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 955 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 1687 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2137 GAATAGTTTC
BbsI 1068 GTCTTC GAAGAC(2/6)
1379 GTCTTC
BcgI 1039 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
212 GCACCAAGATCG
BpuAI 1068 GTCTTC GAAGAC(2/6)
1379 GTCTTC
BsaI 4071 GAGACC GGTCTC(1/5)
409 GGTCTC
BsaWI 2727 TCCGGT [AT]^CCGG[AT]
3551 ACCGGT
BsmBI 2024 CGTCTC CGTCTC(1/5)
759 GAGACG
Bsp1407I 103 TGTACA T^GTACA
1779 TGTACA
BspHI 6 TCATGA T^CATGA
3659 TCATGA
BsrGI 103 TGTACA T^GTACA
1779 TGTACA
BssSI 3612 CACGAG CACGAG(-5/-1)
2396 CTCGTG
DrdI 1059 GACTTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1344 GACCCCCATGTC
HgaI 761 GACGC GACGC(5/10)
2023 GCGTC
HincII 159 GTCAAC GT[CT]^[GA]AC
2718 GTCGAC
HindII 159 GTCAAC GT[CT]^[GA]AC
2718 GTCGAC
RcaI 6 TCATGA T^CATGA
3659 TCATGA
SspBI 103 TGTACA T^GTACA
1779 TGTACA
StuI 1259 AGGCCT AGG^CCT
3189 AGGCCT
TfiI 3106 GAATC G^A[AT]TC
3939 GAATC
XcmI 2056 CCACCTGATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2608 CCAGCAGGCTCTTGG
XmnI 1687 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2137 GAATAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmlI Sse8387I StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII