##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI BbeI BcgI
BfrI BglII BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRV
FseI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NarI NdeI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2402 GTAGAC GT^[AC][GT]AC
AccIII 1971 TCCGGA T^CCGGA
AcsI 1419 GAATTC [GA]^AATT[CT]
ApoI 1419 GAATTC [GA]^AATT[CT]
AviII 187 TGCGCA TGC^GCA
BbvCI 2699 GCTGAGG CCTCAGC(-5/-2)
BciVI 2753 GGATAC GTATCC(6/5)
BclI 787 TGATCA T^GATCA
BsaBI 2638 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1971 TCCGGA [AT]^CCGG[AT]
BseAI 1971 TCCGGA T^CCGGA
BsiEI 1927 CGGCCG CG[GA][CT]^CG
BspEI 1971 TCCGGA T^CCGGA
BspHI 1462 TCATGA T^CATGA
BsrDI 1284 GCAATG GCAATG(2/0)
BssSI 2253 CTCGTG CACGAG(-5/-1)
BstEII 2705 GGTCACC G^GT[ACGT]ACC
BstPI 2705 GGTCACC G^GT[ACGT]ACC
EagI 1927 CGGCCG C^GGCCG
EclXI 1927 CGGCCG C^GGCCG
Eco52I 1927 CGGCCG C^GGCCG
EcoO65I 2705 GGTCACC G^GT[ACGT]ACC
EcoRI 1419 GAATTC G^AATTC
EcoT22I 4199 ATGCAT ATGCA^T
FbaI 787 TGATCA T^GATCA
FspI 187 TGCGCA TGC^GCA
HaeII 2988 AGCGCC [GA]GCGC^[CT]
HindIII 3685 AAGCTT A^AGCTT
MamI 2638 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1971 TCCGGA T^CCGGA
NheI 2 GCTAGC G^CTAGC
NsiI 4199 ATGCAT ATGCA^T
RcaI 1462 TCATGA T^CATGA
SbfI 1847 CCTGCAGG CCTGCA^GG
SexAI 3190 ACCTGGT A^CC[AT]GGT
SphI 2982 GCATGC GCATG^C
Sse8387I 1847 CCTGCAGG CCTGCA^GG
XbaI 3325 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 2840 GACGCC G[GA]^CG[CT]C
3070 GGCGTC
AflIII 1467 ACACGT A^C[GA][CT]GT
1469 ACGTGT
Alw44I 2414 GTGCAC G^TGCAC
2960 GTGCAC
ApaLI 2414 GTGCAC G^TGCAC
2960 GTGCAC
Asp700I 2266 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3394 GAACAGATTC
AspI 1865 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2666 GACCTAGTC
BamHI 2602 GGATCC G^GATCC
2884 GGATCC
BbrPI 1468 CACGTG CAC^GTG
2045 CACGTG
BlpI 125 GCTCAGC GC^T[ACGT]AGC
2071 GCTCAGC
Bpu1102I 125 GCTCAGC GC^T[ACGT]AGC
2071 GCTCAGC
BsaAI 1468 CACGTG [CT]AC^GT[GA]
2045 CACGTG
BsaHI 2840 GACGCC G[GA]^CG[CT]C
3070 GGCGTC
BsaI 312 GAGACC GGTCTC(1/5)
1155 GAGACC
BsmI 70 GCATTC GAATGC(1/-1)
1207 GCATTC
BspMI 1097 ACCTGC ACCTGC(4/8)
3661 ACCTGC
CelII 125 GCTCAGC GC^T[ACGT]AGC
2071 GCTCAGC
DraIII 1219 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2718 CACTGGGTG
EcoNI 3566 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3987 CCTGACTGAGG
Hin1I 2840 GACGCC G[GA]^CG[CT]C
3070 GGCGTC
MseI 639 TTAA T^TAA
3490 TTAA
NaeI 1925 GCCGGC GCC^GGC
2358 GCCGGC
NgoMIV 1925 GCCGGC G^CCGGC
2358 GCCGGC
PaeR7I 1538 CTCGAG C^TCGAG
3389 CTCGAG
PflFI 1865 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2666 GACCTAGTC
PmaCI 1468 CACGTG CAC^GTG
2045 CACGTG
PmlI 1468 CACGTG CAC^GTG
2045 CACGTG
PvuII 2363 CAGCTG CAG^CTG
3116 CAGCTG
SapI 2015 GAAGAGC GCTCTTC(1/4)
2864 GCTCTTC
TaqI 1539 TCGA T^CGA
3390 TCGA
Tru9I 639 TTAA T^TAA
3490 TTAA
Tsp509I 1267 AATT ^AATT
1420 AATT
Tth111I 1865 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2666 GACCTAGTC
TthHB8I 1539 TCGA T^CGA
3390 TCGA
XhoI 1538 CTCGAG C^TCGAG
3389 CTCGAG
XmnI 2266 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3394 GAACAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TfiI
TseI Tsp45I TspRI Van91I XcmI
XhoII XmaCI XmaI