##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BclI BlnI
BlpI Bpu1102I BsaAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2620 TCCGGA T^CCGGA
AclI 2310 AACGTT AA^CGTT
AflII 1746 CTTAAG C^TTAAG
AflIII 220 ACATGT A^C[GA][CT]GT
AlwNI 3070 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3216 CCTGAGG CC^T[ACGT]AGG
BamHI 3210 GGATCC G^GATCC
BanI 179 GGTGCC G^G[CT][GA]CC
BcgI 3339 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1746 CTTAAG C^TTAAG
BsaI 408 GGTCTC GGTCTC(1/5)
BseAI 2620 TCCGGA T^CCGGA
BspEI 2620 TCCGGA T^CCGGA
BspLU11I 220 ACATGT A^CATGT
BspMI 23 ACCTGC ACCTGC(4/8)
BsrDI 2323 GCAATG GCAATG(2/0)
BssSI 4265 CTCGTG CACGAG(-5/-1)
BstAPI 3101 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1276 GGTGACC G^GT[ACGT]ACC
BstPI 1276 GGTGACC G^GT[ACGT]ACC
Bsu36I 3216 CCTGAGG CC^T[ACGT]AGG
DraIII 1671 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1742 GACTCTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3216 CCTGAGG CC^T[ACGT]AGG
EcoNI 3098 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1276 GGTGACC G^GT[ACGT]ACC
HincII 1574 GTCAAC GT[CT]^[GA]AC
HindII 1574 GTCAAC GT[CT]^[GA]AC
MroI 2620 TCCGGA T^CCGGA
NcoI 4190 CCATGG C^CATGG
NruI 2925 TCGCGA TCG^CGA
PflMI 4190 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2310 AACGTT AA^CGTT
SapI 2732 GAAGAGC GCTCTTC(1/4)
SexAI 1712 ACCTGGT A^CC[AT]GGT
SspI 2446 AATATT AAT^ATT
Van91I 4190 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 130 GACGCC G[GA]^CG[CT]C
1834 GACGCC
Alw44I 3908 GTGCAC G^TGCAC
4365 GTGCAC
ApaLI 3908 GTGCAC G^TGCAC
4365 GTGCAC
AspI 963 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3728 GACCTTGTC
BalI 5 TGGCCA TGG^CCA
3243 TGGCCA
BciVI 3428 GTATCC GTATCC(6/5)
3559 GTATCC
BglI 3245 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4207 GCCTCCGAGGC
BsaBI 1339 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1366 GATCGGGATC
BsaHI 130 GACGCC G[GA]^CG[CT]C
1834 GACGCC
BsaWI 2620 TCCGGA [AT]^CCGG[AT]
4134 ACCGGA
DraI 688 TTTAAA TTT^AAA
4221 TTTAAA
EaeI 5 TGGCCA [CT]^GGCC[GA]
3243 TGGCCA
EcoT14I 1126 CCTTGG C^C[AT][AT]GG
4190 CCATGG
FauI 2535 GCGGG CCCGC(4/6)
3723 GCGGG
HgaI 130 GACGC GACGC(5/10)
1834 GACGC
Hin1I 130 GACGCC G[GA]^CG[CT]C
1834 GACGCC
MamI 1339 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1366 GATCGGGATC
MluNI 5 TGGCCA TGG^CCA
3243 TGGCCA
MscI 5 TGGCCA TGG^CCA
3243 TGGCCA
PflFI 963 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3728 GACCTTGTC
SacI 139 GAGCTC GAGCT^C
2735 GAGCTC
SmaI 175 CCCGGG CCC^GGG
3357 CCCGGG
SmlI 1746 CTTAAG C^T[CT][GA]AG
1829 CTCAAG
StuI 2830 AGGCCT AGG^CCT
3171 AGGCCT
StyI 1126 CCTTGG C^C[AT][AT]GG
4190 CCATGG
Tth111I 963 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3728 GACCTTGTC
XbaI 2237 TCTAGA T^CTAGA
3130 TCTAGA
XmaCI 175 CCCGGG C^CCGGG
3357 CCCGGG
XmaI 175 CCCGGG C^CCGGG
3357 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI