################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI AscI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BcgI BclI BglII BlnI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI FspI HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AclI 2770 AACGTT AA^CGTT AcyI 4157 GGCGTC G[GA]^CG[CT]C AflII 4075 CTTAAG C^TTAAG Alw44I 2536 GTGCAC G^TGCAC Aor51HI 1223 AGCGCT AGC^GCT ApaLI 2536 GTGCAC G^TGCAC AseI 1309 ATTAAT AT^TAAT AsnI 1309 ATTAAT AT^TAAT AspI 4488 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 2300 GTCTTC GAAGAC(2/6) BciVI 97 GGATAC GTATCC(6/5) BfrI 4075 CTTAAG C^TTAAG BglI 1662 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 2300 GTCTTC GAAGAC(2/6) BsaAI 3788 TACGTG [CT]AC^GT[GA] BsaBI 4184 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4157 GGCGTC G[GA]^CG[CT]C BsaI 4463 GAGACC GGTCTC(1/5) Bsp1407I 4363 TGTACA T^GTACA BsrFI 201 GCCGGT [GA]^CCGG[CT] BsrGI 4363 TGTACA T^GTACA Cfr10I 201 GCCGGT [GA]^CCGG[CT] DraI 2849 TTTAAA TTT^AAA DraIII 3405 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 47 GACTTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1223 AGCGCT AGC^GCT EcoT22I 3758 ATGCAT ATGCA^T Hin1I 4157 GGCGTC G[GA]^CG[CT]C HindIII 2858 AAGCTT A^AGCTT MamI 4184 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 3734 GCTAGC G^CTAGC NsiI 3758 ATGCAT ATGCA^T PaeR7I 1613 CTCGAG C^TCGAG PflFI 4488 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1309 ATTAAT AT^TAAT Psp1406I 2770 AACGTT AA^CGTT SbfI 132 CCTGCAGG CCTGCA^GG Sse8387I 132 CCTGCAGG CCTGCA^GG SspBI 4363 TGTACA T^GTACA StuI 2081 AGGCCT AGG^CCT Tth111I 4488 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1613 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 1895 CGCG CG^CG 3270 CGCG AcsI 1775 GAATTC [GA]^AATT[CT] 2623 GAATTC AflIII 1376 ACATGT A^C[GA][CT]GT 3703 ACATGT ApoI 1775 GAATTC [GA]^AATT[CT] 2623 GAATTC BamHI 1301 GGATCC G^GATCC 1965 GGATCC BlpI 423 GCTGAGC GC^T[ACGT]AGC 1817 GCTCAGC Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC 1817 GCTCAGC BspLU11I 1376 ACATGT A^CATGT 3703 ACATGT BspMI 478 ACCTGC ACCTGC(4/8) 3530 ACCTGC BstEII 2403 GGTCACC G^GT[ACGT]ACC 2467 GGTTACC BstPI 2403 GGTCACC G^GT[ACGT]ACC 2467 GGTTACC BstUI 1895 CGCG CG^CG 3270 CGCG CelII 423 GCTGAGC GC^T[ACGT]AGC 1817 GCTCAGC EcoO65I 2403 GGTCACC G^GT[ACGT]ACC 2467 GGTTACC EcoRI 1775 GAATTC G^AATTC 2623 GAATTC HaeII 1223 AGCGCT [GA]GCGC^[CT] 1451 AGCGCC MvnI 1895 CGCG CG^CG 3270 CGCG NcoI 1092 CCATGG C^CATGG 2181 CCATGG SapI 2867 GAAGAGC GCTCTTC(1/4) 3251 GAAGAGC SmaI 3066 CCCGGG CCC^GGG 4017 CCCGGG TaqI 1614 TCGA T^CGA 2488 TCGA TthHB8I 1614 TCGA T^CGA 2488 TCGA XmaCI 3066 CCCGGG C^CCGGG 4017 CCCGGG XmaI 3066 CCCGGG C^CCGGG 4017 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII