##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AclI AgeI AocI Aor51HI
AscI Asp718I AviII BamHI BbeI
BcgI BglII BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrFI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HpaI
KasI KpnI KspI MfeI MluI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4182 GACGTC GACGT^C
AccI 1509 GTCTAC GT^[AC][GT]AC
AccIII 2019 TCCGGA T^CCGGA
AcyI 4182 GACGTC G[GA]^CG[CT]C
AflII 3533 CTTAAG C^TTAAG
AhdI 4588 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 628 GTGCAC G^TGCAC
ApaLI 628 GTGCAC G^TGCAC
Asp700I 423 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4588 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1001 CCTAGG C^CTAGG
BbrPI 2950 CACGTG CAC^GTG
BbvCI 1911 CCTCAGC CCTCAGC(-5/-2)
BfrI 3533 CTTAAG C^TTAAG
BglI 1671 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1001 CCTAGG C^CTAGG
BsaAI 2950 CACGTG [CT]AC^GT[GA]
BsaBI 900 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4182 GACGTC G[GA]^CG[CT]C
BseAI 2019 TCCGGA T^CCGGA
BsmI 1682 GAATGC GAATGC(1/-1)
BspEI 2019 TCCGGA T^CCGGA
BssHII 1621 GCGCGC G^CGCGC
BstAPI 1875 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 3101 GGATCT [GA]^GATC[CT]
Eam1105I 4588 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 591 GAATTC G^AATTC
HaeII 1856 GGCGCT [GA]GCGC^[CT]
HgaI 1591 GACGC GACGC(5/10)
Hin1I 4182 GACGTC G[GA]^CG[CT]C
HincII 2787 GTCAAC GT[CT]^[GA]AC
HindII 2787 GTCAAC GT[CT]^[GA]AC
MamI 900 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3101 GGATCT [GA]^GATC[CT]
MroI 2019 TCCGGA T^CCGGA
PmaCI 2950 CACGTG CAC^GTG
PmlI 2950 CACGTG CAC^GTG
TaqI 2013 TCGA T^CGA
TthHB8I 2013 TCGA T^CGA
XhoII 3101 GGATCT [GA]^GATC[CT]
XmnI 423 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1622 CGCG CG^CG
2082 CGCG
AlwI 2031 GATCC GGATC(4/5)
3101 GGATC
AlwNI 2617 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
4247 CAGGGTCTG
AvaI 1920 CCCGAG C^[CT]CG[GA]G
2823 CTCGGG
BalI 2433 TGGCCA TGG^CCA
2459 TGGCCA
BciVI 3397 GGATAC GTATCC(6/5)
3128 GTATCC
BclI 1007 TGATCA T^GATCA
3267 TGATCA
BcnI 2086 CCCGG CC^[GC]GG
2660 CCCGG
BlpI 3315 GCTTAGC GC^T[ACGT]AGC
4529 GCTCAGC
Bpu1102I 3315 GCTTAGC GC^T[ACGT]AGC
4529 GCTCAGC
BsaI 914 GAGACC GGTCTC(1/5)
352 GGTCTC
BsaWI 2019 TCCGGA [AT]^CCGG[AT]
2179 TCCGGT
BsoBI 1920 CCCGAG C^[CT]CG[GA]G
2823 CTCGGG
BspLU11I 2406 ACATGT A^CATGT
4427 ACATGT
BsrDI 1267 CATTGC GCAATG(2/0)
4215 GCAATG
BstUI 1622 CGCG CG^CG
2082 CGCG
CelII 3315 GCTTAGC GC^T[ACGT]AGC
4529 GCTCAGC
DraI 690 TTTAAA TTT^AAA
1452 TTTAAA
EaeI 2433 TGGCCA [CT]^GGCC[GA]
2459 TGGCCA
FauI 1307 CCCGC CCCGC(4/6)
1602 GCGGG
FbaI 1007 TGATCA T^GATCA
3267 TGATCA
HindIII 1069 AAGCTT A^AGCTT
3820 AAGCTT
MluNI 2433 TGGCCA TGG^CCA
2459 TGGCCA
MscI 2433 TGGCCA TGG^CCA
2459 TGGCCA
MvnI 1622 CGCG CG^CG
2082 CGCG
NciI 2086 CCCGG CC^[GC]GG
2660 CCCGG
SapI 1492 GAAGAGC GCTCTTC(1/4)
2143 GAAGAGC
SspI 3483 AATATT AAT^ATT
3653 AATATT
StuI 813 AGGCCT AGG^CCT
1348 AGGCCT
XcmI 648 CCATCCCTTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2389 CCATTTGAGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApaI ApoI AseI AsnI AspHI
AspI AvaII BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspHI BspMI BsrI BssKI BssSI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PshBI RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XbaI