##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AflIII Alw44I Aor51HI
ApaLI AscI Asp718I AviII AvrII
BbsI BlnI BpuAI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI EcoRV
FspI KpnI KspI MamI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NspV PacI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SfiI SfuI SnaBI SpeI
SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 3272 GACGTC GACGT^C
AccI 2297 GTAGAC GT^[AC][GT]AC
AccIII 815 TCCGGA T^CCGGA
AcsI 2739 GAATTT [GA]^AATT[CT]
AgeI 1807 ACCGGT A^CCGGT
AhdI 2047 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2739 GAATTT [GA]^AATT[CT]
AseI 1096 ATTAAT AT^TAAT
AsnI 1096 ATTAAT AT^TAAT
Asp700I 2903 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2047 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1116 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 310 GGATCC G^GATCC
BbeI 3024 GGCGCC GGCGC^C
BbrPI 2029 CACGTG CAC^GTG
BbvCI 1209 CCTCAGC CCTCAGC(-5/-2)
BcgI 1198 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2591 GTATCC GTATCC(6/5)
BclI 1570 TGATCA T^GATCA
BsaAI 2029 CACGTG [CT]AC^GT[GA]
BseAI 815 TCCGGA T^CCGGA
BsiEI 3133 CGGCCG CG[GA][CT]^CG
BspEI 815 TCCGGA T^CCGGA
BsrDI 1149 GCAATG GCAATG(2/0)
BssHII 1643 GCGCGC G^CGCGC
BssSI 3690 CTCGTG CACGAG(-5/-1)
BstEII 2044 GGTGACC G^GT[ACGT]ACC
BstPI 2044 GGTGACC G^GT[ACGT]ACC
DraIII 637 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3054 GACGGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3133 CGGCCG C^GGCCG
Eam1105I 2047 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3133 CGGCCG C^GGCCG
Eco52I 3133 CGGCCG C^GGCCG
EcoO65I 2044 GGTGACC G^GT[ACGT]ACC
FbaI 1570 TGATCA T^GATCA
HincII 1001 GTTAAC GT[CT]^[GA]AC
HindII 1001 GTTAAC GT[CT]^[GA]AC
HindIII 98 AAGCTT A^AGCTT
HpaI 1001 GTTAAC GTT^AAC
KasI 3024 GGCGCC G^GCGCC
MroI 815 TCCGGA T^CCGGA
NarI 3024 GGCGCC GG^CGCC
PaeR7I 4105 CTCGAG C^TCGAG
PflFI 1116 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1807 ACCGGT A^CCGGT
PmaCI 2029 CACGTG CAC^GTG
PmlI 2029 CACGTG CAC^GTG
PshAI 3268 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1096 ATTAAT AT^TAAT
SapI 204 GAAGAGC GCTCTTC(1/4)
SbfI 841 CCTGCAGG CCTGCA^GG
ScaI 4424 AGTACT AGT^ACT
SexAI 40 ACCTGGT A^CC[AT]GGT
SfoI 3024 GGCGCC GGC^GCC
SgrAI 1806 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 841 CCTGCAGG CCTGCA^GG
SspI 4522 AATATT AAT^ATT
StuI 1959 AGGCCT AGG^CCT
Tth111I 1116 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4105 CTCGAG C^TCGAG
XmnI 2903 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 3024 GGCGCC G[GA]^CG[CT]C
3272 GACGTC
AflII 1262 CTTAAG C^TTAAG
2323 CTTAAG
BfrI 1262 CTTAAG C^TTAAG
2323 CTTAAG
BglII 791 AGATCT A^GATCT
2004 AGATCT
BsaHI 3024 GGCGCC G[GA]^CG[CT]C
3272 GACGTC
BsmI 1029 GAATGC GAATGC(1/-1)
2907 GCATTC
DsaI 3618 CCACGG C^C[GA][CT]GG
4251 CCACGG
EcoT22I 1015 ATGCAT ATGCA^T
1031 ATGCAT
FseI 2249 GGCCGGCC GGCCGG^CC
2849 GGCCGGCC
Hin1I 3024 GGCGCC G[GA]^CG[CT]C
3272 GACGTC
NsiI 1015 ATGCAT ATGCA^T
1031 ATGCAT
PflMI 416 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3176 CCAAGGGCTGG
SmaI 1952 CCCGGG CCC^GGG
3465 CCCGGG
SphI 1597 GCATGC GCATG^C
4135 GCATGC
Van91I 416 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3176 CCAAGGGCTGG
XmaCI 1952 CCCGGG C^CCGGG
3465 CCCGGG
XmaI 1952 CCCGGG C^CCGGG
3465 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII