##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AviII AvrII BamHI BbrPI
BclI BcnI BlnI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FbaI FseI FspI HapII
HpaI HpaII KpnI KspI MfeI
MluI MroI MspI MunI NaeI
NciI NdeI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 4430 GTCTAC GT^[AC][GT]AC
AflII 1519 CTTAAG C^TTAAG
Aor51HI 3681 AGCGCT AGC^GCT
Asp700I 3329 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1734 CTCGAG C^[CT]CG[GA]G
BalI 2806 TGGCCA TGG^CCA
BbeI 185 GGCGCC GGCGC^C
BcgI 452 CGAAAGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3383 GTATCC GTATCC(6/5)
BfrI 1519 CTTAAG C^TTAAG
BglII 2212 AGATCT A^GATCT
BlpI 755 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 755 GCTGAGC GC^T[ACGT]AGC
BsaBI 2168 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1734 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1029 TGTACA T^GTACA
BsrBI 552 CCGCTC CCGCTC(-3/-3)
BsrDI 2722 GCAATG GCAATG(2/0)
BsrGI 1029 TGTACA T^GTACA
BstAPI 2790 GCAGTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 755 GCTGAGC GC^T[ACGT]AGC
Eco47III 3681 AGCGCT AGC^GCT
HincII 897 GTTGAC GT[CT]^[GA]AC
HindII 897 GTTGAC GT[CT]^[GA]AC
KasI 185 GGCGCC G^GCGCC
MamI 2168 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2806 TGGCCA TGG^CCA
MscI 2806 TGGCCA TGG^CCA
MspA1I 936 CCGCTG C[AC]G^C[GT]G
NarI 185 GGCGCC GG^CGCC
NheI 1003 GCTAGC G^CTAGC
PaeR7I 1734 CTCGAG C^TCGAG
PpuMI 312 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2435 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 456 AGTACT AGT^ACT
SfoI 185 GGCGCC GGC^GCC
SpeI 4264 ACTAGT A^CTAGT
SspBI 1029 TGTACA T^GTACA
XbaI 2651 TCTAGA T^CTAGA
XhoI 1734 CTCGAG C^TCGAG
XmnI 3329 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 22 CGCG CG^CG
1690 CGCG
AcyI 185 GGCGCC G[GA]^CG[CT]C
268 GACGCC
AocI 394 CCTCAGG CC^T[ACGT]AGG
1362 CCTCAGG
AseI 2974 ATTAAT AT^TAAT
3562 ATTAAT
AsnI 2974 ATTAAT AT^TAAT
3562 ATTAAT
AspI 268 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1643 GACCTTGTC
BbsI 1492 GAAGAC GAAGAC(2/6)
3914 GAAGAC
BbvCI 1718 GCTGAGG CCTCAGC(-5/-2)
3147 GCTGAGG
BpuAI 1492 GAAGAC GAAGAC(2/6)
3914 GAAGAC
BsaAI 1200 CACGTA [CT]AC^GT[GA]
3380 CACGTA
BsaHI 185 GGCGCC G[GA]^CG[CT]C
268 GACGCC
BsmI 4365 GAATGC GAATGC(1/-1)
3212 GCATTC
BspLU11I 3011 ACATGT A^CATGT
3472 ACATGT
BstUI 22 CGCG CG^CG
1690 CGCG
Bsu36I 394 CCTCAGG CC^T[ACGT]AGG
1362 CCTCAGG
DraIII 1245 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1299 CACCTAGTG
EaeI 2806 TGGCCA [CT]^GGCC[GA]
3602 TGGCCG
Eco81I 394 CCTCAGG CC^T[ACGT]AGG
1362 CCTCAGG
FauI 41 CCCGC CCCGC(4/6)
466 GCGGG
HaeII 185 GGCGCC [GA]GCGC^[CT]
3681 AGCGCT
HgaI 268 GACGC GACGC(5/10)
1691 GCGTC
Hin1I 185 GGCGCC G[GA]^CG[CT]C
268 GACGCC
HindIII 2023 AAGCTT A^AGCTT
3398 AAGCTT
MvnI 22 CGCG CG^CG
1690 CGCG
PflFI 268 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1643 GACCTTGTC
PshBI 2974 ATTAAT AT^TAAT
3562 ATTAAT
SapI 3731 GAAGAGC GCTCTTC(1/4)
2490 GCTCTTC
SspI 4072 AATATT AAT^ATT
4318 AATATT
StuI 846 AGGCCT AGG^CCT
3413 AGGCCT
Tth111I 268 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1643 GACCTTGTC
XcmI 584 CCACGGTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4361 CCATGAATGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BanI BanII BbvI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII