##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccIII AgeI Aor51HI
AseI AsnI Asp718I AviII BbrPI
BcgI BclI BglII BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 4701 GACGTC GACGT^C
AflII 3314 CTTAAG C^TTAAG
AhdI 1309 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3470 GTGCAC G^TGCAC
ApaLI 3470 GTGCAC G^TGCAC
AscI 1769 GGCGCGCC GG^CGCGCC
Asp700I 1536 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1309 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 3314 CTTAAG C^TTAAG
BsaAI 3958 CACGTA [CT]AC^GT[GA]
BsiEI 4234 CGGCCG CG[GA][CT]^CG
BspLU11I 4597 ACATGT A^CATGT
BsrBI 1404 CCGCTC CCGCTC(-3/-3)
BsrDI 838 GCAATG GCAATG(2/0)
BssHII 1770 GCGCGC G^CGCGC
DraI 337 TTTAAA TTT^AAA
EagI 4234 CGGCCG C^GGCCG
Eam1105I 1309 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4234 CGGCCG C^GGCCG
Eco52I 4234 CGGCCG C^GGCCG
HincII 1721 GTTGAC GT[CT]^[GA]AC
HindII 1721 GTTGAC GT[CT]^[GA]AC
NheI 2347 GCTAGC G^CTAGC
PaeR7I 15 CTCGAG C^TCGAG
PflMI 2220 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1988 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 3583 AGTACT AGT^ACT
SexAI 1012 ACCAGGT A^CC[AT]GGT
SphI 3168 GCATGC GCATG^C
SwaI 336 ATTTAAAT ATTT^AAAT
Van91I 2220 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 15 CTCGAG C^TCGAG
XmnI 1536 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 3294 AACGTT AA^CGTT
4798 AACGTT
AcsI 526 AAATTT [GA]^AATT[CT]
2001 AAATTT
ApoI 526 AAATTT [GA]^AATT[CT]
2001 AAATTT
AspI 2635 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4663 GACTGTGTC
BamHI 222 GGATCC G^GATCC
1737 GGATCC
BbeI 25 GGCGCC GGCGC^C
2062 GGCGCC
BciVI 1894 GGATAC GTATCC(6/5)
2521 GGATAC
BsaI 1064 GAGACC GGTCTC(1/5)
2572 GAGACC
BspHI 672 TCATGA T^CATGA
4468 TCATGA
BssSI 3595 CACGAG CACGAG(-5/-1)
3510 CTCGTG
BstAPI 1092 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2269 GCACTACCTGC
BstXI 1647 CCAGTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3496 CCACCTGAGTGG
KasI 25 GGCGCC G^GCGCC
2062 GGCGCC
NaeI 28 GCCGGC GCC^GGC
1475 GCCGGC
NarI 25 GGCGCC GG^CGCC
2062 GGCGCC
NcoI 1632 CCATGG C^CATGG
1765 CCATGG
NgoMIV 28 GCCGGC G^CCGGC
1475 GCCGGC
PflFI 2635 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4663 GACTGTGTC
Psp1406I 3294 AACGTT AA^CGTT
4798 AACGTT
RcaI 672 TCATGA T^CATGA
4468 TCATGA
SacI 2857 GAGCTC GAGCT^C
4464 GAGCTC
SbfI 4342 CCTGCAGG CCTGCA^GG
4369 CCTGCAGG
SfoI 25 GGCGCC GGC^GCC
2062 GGCGCC
Sse8387I 4342 CCTGCAGG CCTGCA^GG
4369 CCTGCAGG
Tth111I 2635 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4663 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI