##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI ApoI AscI Asp700I
Asp718I AvrII BciVI BclI BfrI
BlnI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
ClaI DraI EcoRI EcoRV EcoT22I
FbaI HpaI KpnI MfeI MluI
MunI NdeI NotI NruI NsiI
NspI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RcaI SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3895 GACGTC GACGT^C
AccIII 1380 TCCGGA T^CCGGA
Alw44I 1716 GTGCAC G^TGCAC
Aor51HI 198 AGCGCT AGC^GCT
ApaLI 1716 GTGCAC G^TGCAC
AseI 2504 ATTAAT AT^TAAT
AsnI 2504 ATTAAT AT^TAAT
AspI 524 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 759 TGGCCA TGG^CCA
BglII 2528 AGATCT A^GATCT
BseAI 1380 TCCGGA T^CCGGA
BsmI 1725 GCATTC GAATGC(1/-1)
BspEI 1380 TCCGGA T^CCGGA
BsrDI 978 GCAATG GCAATG(2/0)
BstEII 496 GGTGACC G^GT[ACGT]ACC
BstPI 496 GGTGACC G^GT[ACGT]ACC
CpoI 458 CGGACCG CG^G[AT]CCG
Eco47III 198 AGCGCT AGC^GCT
EcoNI 3825 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 496 GGTGACC G^GT[ACGT]ACC
HincII 3892 GTTGAC GT[CT]^[GA]AC
HindII 3892 GTTGAC GT[CT]^[GA]AC
KspI 3182 CCGCGG CCGC^GG
MluNI 759 TGGCCA TGG^CCA
MroI 1380 TCCGGA T^CCGGA
MscI 759 TGGCCA TGG^CCA
NheI 1040 GCTAGC G^CTAGC
PaeR7I 1760 CTCGAG C^TCGAG
PflFI 524 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2504 ATTAAT AT^TAAT
RsrII 458 CGGACCG CG^G[AT]CCG
SacII 3182 CCGCGG CCGC^GG
SapI 3165 GCTCTTC GCTCTTC(1/4)
SmlI 1760 CTCGAG C^T[CT][GA]AG
SspI 2468 AATATT AAT^ATT
Tth111I 524 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2120 TCTAGA T^CTAGA
XhoI 1760 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AhdI 2006 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3208 GACCTGGTGTC
AspEI 2006 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3208 GACCTGGTGTC
BamHI 1027 GGATCC G^GATCC
3938 GGATCC
BbrPI 1713 CACGTG CAC^GTG
1845 CACGTG
BbsI 517 GAAGAC GAAGAC(2/6)
2375 GTCTTC
BbvCI 2402 CCTCAGC CCTCAGC(-5/-2)
3377 CCTCAGC
BpuAI 517 GAAGAC GAAGAC(2/6)
2375 GTCTTC
BsaAI 1713 CACGTG [CT]AC^GT[GA]
1845 CACGTG
BsaBI 2529 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4024 GATGACGATC
BsaI 1449 GGTCTC GGTCTC(1/5)
3118 GGTCTC
BsaWI 1380 TCCGGA [AT]^CCGG[AT]
4012 ACCGGA
BsmBI 1906 CGTCTC CGTCTC(1/5)
2593 CGTCTC
BssHII 147 GCGCGC G^CGCGC
2954 GCGCGC
BssSI 1164 CTCGTG CACGAG(-5/-1)
2582 CTCGTG
BstXI 595 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4107 CCAGGTGCCTGG
DraIII 1365 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1842 CACCACGTG
DrdI 2265 GACTGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3741 GACACTGGGGTC
Eam1105I 2006 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3208 GACCTGGTGTC
FseI 3149 GGCCGGCC GGCCGG^CC
3706 GGCCGGCC
HindIII 1375 AAGCTT A^AGCTT
2832 AAGCTT
MamI 2529 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4024 GATGACGATC
MseI 2505 TTAA T^TAA
4145 TTAA
MslI 2016 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3496 CATTTGCATG
PmaCI 1713 CACGTG CAC^GTG
1845 CACGTG
PmlI 1713 CACGTG CAC^GTG
1845 CACGTG
PvuII 1277 CAGCTG CAG^CTG
3233 CAGCTG
SacI 752 GAGCTC GAGCT^C
1010 GAGCTC
SexAI 3209 ACCTGGT A^CC[AT]GGT
3443 ACCTGGT
SmaI 831 CCCGGG CCC^GGG
4040 CCCGGG
StuI 1764 AGGCCT AGG^CCT
3348 AGGCCT
TfiI 192 GAATC G^A[AT]TC
961 GATTC
Tru9I 2505 TTAA T^TAA
4145 TTAA
XmaCI 831 CCCGGG C^CCGGG
4040 CCCGGG
XmaI 831 CCCGGG C^CCGGG
4040 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AviII BanI BanII
BbeI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII