##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AclI AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BcgI BglII BsiWI
BspDI BsrDI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfuI SgrAI
SnaBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2774 GACGTC GACGT^C
AccI 1802 GTATAC GT^[AC][GT]AC
AcsI 1215 GAATTT [GA]^AATT[CT]
AflII 2873 CTTAAG C^TTAAG
AocI 826 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1367 AGCGCT AGC^GCT
ApoI 1215 GAATTT [GA]^AATT[CT]
BbrPI 2789 CACGTG CAC^GTG
BciVI 2171 GGATAC GTATCC(6/5)
BclI 17 TGATCA T^GATCA
BfrI 2873 CTTAAG C^TTAAG
BsaAI 2789 CACGTG [CT]AC^GT[GA]
BsaBI 1349 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2353 GCATTC GAATGC(1/-1)
BspLU11I 1277 ACATGT A^CATGT
Bst1107I 1802 GTATAC GTA^TAC
BstZ17I 1802 GTATAC GTA^TAC
Bsu36I 826 CCTTAGG CC^T[ACGT]AGG
Eco47III 1367 AGCGCT AGC^GCT
Eco81I 826 CCTTAGG CC^T[ACGT]AGG
FbaI 17 TGATCA T^GATCA
MamI 1349 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 4289 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 976 GCCGGC GCC^GGC
NgoMIV 976 GCCGGC G^CCGGC
PmaCI 2789 CACGTG CAC^GTG
PmlI 2789 CACGTG CAC^GTG
SacI 1385 GAGCTC GAGCT^C
SbfI 327 CCTGCAGG CCTGCA^GG
SpeI 1806 ACTAGT A^CTAGT
SphI 657 GCATGC GCATG^C
Sse8387I 327 CCTGCAGG CCTGCA^GG
TaqI 2922 TCGA T^CGA
TthHB8I 2922 TCGA T^CGA
XcmI 3471 CCATCTTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 2778 TCCGGA T^CCGGA
3510 TCCGGA
AvrII 1087 CCTAGG C^CTAGG
3097 CCTAGG
BamHI 3591 GGATCC G^GATCC
4094 GGATCC
BbeI 2840 GGCGCC GGCGC^C
3541 GGCGCC
BlnI 1087 CCTAGG C^CTAGG
3097 CCTAGG
BsaWI 2778 TCCGGA [AT]^CCGG[AT]
3510 TCCGGA
BseAI 2778 TCCGGA T^CCGGA
3510 TCCGGA
BsiEI 3552 CGGCCG CG[GA][CT]^CG
3836 CGGCCG
BsmBI 96 CGTCTC CGTCTC(1/5)
3690 GAGACG
Bsp1407I 1252 TGTACA T^GTACA
2944 TGTACA
BspEI 2778 TCCGGA T^CCGGA
3510 TCCGGA
BsrBI 584 CCGCTC CCGCTC(-3/-3)
3338 GAGCGG
BsrGI 1252 TGTACA T^GTACA
2944 TGTACA
BstAPI 593 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3046 GCATCTGCTGC
EagI 3552 CGGCCG C^GGCCG
3836 CGGCCG
EarI 3449 CTCTTC CTCTTC(1/4)
4028 GAAGAG
EclXI 3552 CGGCCG C^GGCCG
3836 CGGCCG
Eco52I 3552 CGGCCG C^GGCCG
3836 CGGCCG
KasI 2840 GGCGCC G^GCGCC
3541 GGCGCC
Ksp632I 3449 CTCTTC CTCTTC(1/4)
4028 GAAGAG
MroI 2778 TCCGGA T^CCGGA
3510 TCCGGA
NarI 2840 GGCGCC GG^CGCC
3541 GGCGCC
NspI 657 GCATGC [GA]CATG^[CT]
1277 ACATGT
SexAI 2487 ACCTGGT A^CC[AT]GGT
2745 ACCAGGT
SfoI 2840 GGCGCC GGC^GCC
3541 GGCGCC
SspBI 1252 TGTACA T^GTACA
2944 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmaCI XmaI