##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccI AclI AgeI AscI
AseI AsnI Asp718I AspI BbrPI
BglI BglII BsaBI BsiWI Bsp1407I
BspDI BspHI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
KspI MamI MluI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1307 GACGTC GACGT^C
AccIII 1622 TCCGGA T^CCGGA
AhdI 3394 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4050 GTGCAC G^TGCAC
Aor51HI 2158 AGCGCT AGC^GCT
ApaLI 4050 GTGCAC G^TGCAC
Asp700I 2028 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3394 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1522 TGCGCA TGC^GCA
AvrII 2377 CCTAGG C^CTAGG
BamHI 1516 GGATCC G^GATCC
BbeI 69 GGCGCC GGCGC^C
BcgI 1919 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2377 CCTAGG C^CTAGG
BsaWI 1622 TCCGGA [AT]^CCGG[AT]
BseAI 1622 TCCGGA T^CCGGA
BsmBI 1974 GAGACG CGTCTC(1/5)
BsmI 732 GAATGC GAATGC(1/-1)
BspEI 1622 TCCGGA T^CCGGA
BsrDI 624 GCAATG GCAATG(2/0)
BssHII 1 GCGCGC G^CGCGC
BstEII 3781 GGTGACC G^GT[ACGT]ACC
BstPI 3781 GGTGACC G^GT[ACGT]ACC
DraI 3021 TTTAAA TTT^AAA
DraIII 720 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1851 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3394 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2158 AGCGCT AGC^GCT
EcoNI 2870 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3781 GGTGACC G^GT[ACGT]ACC
FspI 1522 TGCGCA TGC^GCA
HincII 2277 GTCAAC GT[CT]^[GA]AC
HindII 2277 GTCAAC GT[CT]^[GA]AC
KasI 69 GGCGCC G^GCGCC
MfeI 3847 CAATTG C^AATTG
MroI 1622 TCCGGA T^CCGGA
MunI 3847 CAATTG C^AATTG
NarI 69 GGCGCC GG^CGCC
NcoI 73 CCATGG C^CATGG
NdeI 2971 CATATG CA^TATG
NheI 374 GCTAGC G^CTAGC
PshAI 1569 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 721 ACCTGGT A^CC[AT]GGT
SfoI 69 GGCGCC GGC^GCC
SpeI 3273 ACTAGT A^CTAGT
StuI 709 AGGCCT AGG^CCT
TaqI 531 TCGA T^CGA
TthHB8I 531 TCGA T^CGA
XcmI 3546 CCAGAATAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2028 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 297 GAATTT [GA]^AATT[CT]
2952 AAATTC
AcyI 69 GGCGCC G[GA]^CG[CT]C
1307 GACGTC
AflIII 2706 ACATGT A^C[GA][CT]GT
2922 ACATGT
ApoI 297 GAATTT [GA]^AATT[CT]
2952 AAATTC
BciVI 869 GGATAC GTATCC(6/5)
2624 GGATAC
BlpI 125 GCTCAGC GC^T[ACGT]AGC
3517 GCTTAGC
Bpu1102I 125 GCTCAGC GC^T[ACGT]AGC
3517 GCTTAGC
BsaAI 888 TACGTG [CT]AC^GT[GA]
1644 TACGTG
BsaHI 69 GGCGCC G[GA]^CG[CT]C
1307 GACGTC
BsiEI 7 CGGCCG CG[GA][CT]^CG
403 CGGCCG
BspLU11I 2706 ACATGT A^CATGT
2922 ACATGT
BsrBI 48 CCGCTC CCGCTC(-3/-3)
420 CCGCTC
BssSI 141 CACGAG CACGAG(-5/-1)
918 CACGAG
BstAPI 197 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
917 GCACGAGCTGC
BstXI 271 CCAGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
715 CCATGCACCTGG
CelII 125 GCTCAGC GC^T[ACGT]AGC
3517 GCTTAGC
EagI 7 CGGCCG C^GGCCG
403 CGGCCG
EclXI 7 CGGCCG C^GGCCG
403 CGGCCG
Eco52I 7 CGGCCG C^GGCCG
403 CGGCCG
Hin1I 69 GGCGCC G[GA]^CG[CT]C
1307 GACGTC
SapI 671 GAAGAGC GCTCTTC(1/4)
1199 GAAGAGC
SmaI 54 CCCGGG CCC^GGG
1481 CCCGGG
XmaCI 54 CCCGGG C^CCGGG
1481 CCCGGG
XmaI 54 CCCGGG C^CCGGG
1481 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AflII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII