##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BclI BfrI
BlnI BlpI Bpu1102I BsiWI BsmI
BspDI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DrdI EcoNI EcoRI EcoRV FbaI
FspI HincII HindII HpaI KpnI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI ScaI SfuI SnaBI SpeI
SphI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 4247 AAATTT [GA]^AATT[CT]
AhdI 3671 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 692 GTGCAC G^TGCAC
Aor51HI 552 AGCGCT AGC^GCT
ApaLI 692 GTGCAC G^TGCAC
ApoI 4247 AAATTT [GA]^AATT[CT]
AspEI 3671 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 809 CACGTG CAC^GTG
BcgI 3505 CGACCTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 495 AGATCT A^GATCT
BsaBI 496 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4228 TGTACA T^GTACA
BsrDI 3775 CATTGC GCAATG(2/0)
BsrGI 4228 TGTACA T^GTACA
BstEII 1737 GGTCACC G^GT[ACGT]ACC
BstPI 1737 GGTCACC G^GT[ACGT]ACC
DraI 4244 TTTAAA TTT^AAA
DraIII 2515 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3671 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 552 AGCGCT AGC^GCT
EcoO65I 1737 GGTCACC G^GT[ACGT]ACC
EcoT22I 1273 ATGCAT ATGCA^T
FseI 161 GGCCGGCC GGCCGG^CC
HindIII 2347 AAGCTT A^AGCTT
KspI 3945 CCGCGG CCGC^GG
MamI 496 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 1273 ATGCAT ATGCA^T
PmaCI 809 CACGTG CAC^GTG
PmlI 809 CACGTG CAC^GTG
SacII 3945 CCGCGG CCGC^GG
SexAI 3960 ACCTGGT A^CC[AT]GGT
SspBI 4228 TGTACA T^GTACA
SspI 4240 AATATT AAT^ATT
SwaI 4243 ATTTAAAT ATTT^AAAT
TfiI 2215 GATTC G^A[AT]TC
Tsp509I 4248 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 304 TCCGGA T^CCGGA
1132 TCCGGA
AflIII 808 ACACGT A^C[GA][CT]GT
810 ACGTGT
AocI 284 CCTGAGG CC^T[ACGT]AGG
1574 CCTGAGG
AspI 1762 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3188 GACACTGTC
BalI 763 TGGCCA TGG^CCA
3142 TGGCCA
BbsI 545 GTCTTC GAAGAC(2/6)
3653 GTCTTC
BbvCI 1944 GCTGAGG CCTCAGC(-5/-2)
2750 GCTGAGG
BciVI 1440 GTATCC GTATCC(6/5)
1798 GTATCC
BpuAI 545 GTCTTC GAAGAC(2/6)
3653 GTCTTC
BsaAI 809 CACGTG [CT]AC^GT[GA]
2072 CACGTA
BsaI 124 GAGACC GGTCTC(1/5)
674 GAGACC
BsaWI 304 TCCGGA [AT]^CCGG[AT]
1132 TCCGGA
BseAI 304 TCCGGA T^CCGGA
1132 TCCGGA
BsiEI 160 CGGCCG CG[GA][CT]^CG
2900 CGGCCG
BsmBI 2163 CGTCTC CGTCTC(1/5)
3613 GAGACG
BspEI 304 TCCGGA T^CCGGA
1132 TCCGGA
BstAPI 2142 GCAGGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3811 GCAGAGACTGC
Bsu36I 284 CCTGAGG CC^T[ACGT]AGG
1574 CCTGAGG
EagI 160 CGGCCG C^GGCCG
2900 CGGCCG
EclXI 160 CGGCCG C^GGCCG
2900 CGGCCG
Eco52I 160 CGGCCG C^GGCCG
2900 CGGCCG
Eco81I 284 CCTGAGG CC^T[ACGT]AGG
1574 CCTGAGG
MluNI 763 TGGCCA TGG^CCA
3142 TGGCCA
MroI 304 TCCGGA T^CCGGA
1132 TCCGGA
MscI 763 TGGCCA TGG^CCA
3142 TGGCCA
MseI 4245 TTAA T^TAA
4276 TTAA
PflFI 1762 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3188 GACACTGTC
PflMI 2765 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3562 CCACAGCCTGG
SbfI 1584 CCTGCAGG CCTGCA^GG
1603 CCTGCAGG
SfiI 896 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3787 GGCCCGCCAGGCC
SgrAI 1300 CGCCGGCG C[GA]^CCGG[CT]G
2920 CGCCGGCG
SmlI 325 CTTGAG C^T[CT][GA]AG
1876 CTTGAG
Sse8387I 1584 CCTGCAGG CCTGCA^GG
1603 CCTGCAGG
Tru9I 4245 TTAA T^TAA
4276 TTAA
Tth111I 1762 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3188 GACACTGTC
Van91I 2765 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3562 CCACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BamHI BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI