##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AflII AgeI AocI
AscI AseI AsnI Asp718I AviII
AvrII BclI BfrI BglII BlnI
BsaBI BsiWI BssHII BstBI Bsu36I
CpoI DrdI Eco81I EcoRI EcoRV
FbaI FseI FspI KpnI MamI
MluI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 991 GACGTC GACGT^C
AccI 103 GTATAC GT^[AC][GT]AC
AcsI 3482 AAATTC [GA]^AATT[CT]
AhdI 2820 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3482 AAATTC [GA]^AATT[CT]
Asp700I 2942 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2820 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 586 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 564 GGATCC G^GATCC
BbeI 4195 GGCGCC GGCGC^C
BbrPI 7 CACGTG CAC^GTG
BcgI 2774 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 4071 CGGCCG CG[GA][CT]^CG
Bsp1407I 1557 TGTACA T^GTACA
BspDI 1963 ATCGAT AT^CGAT
BspHI 1439 TCATGA T^CATGA
BsrGI 1557 TGTACA T^GTACA
Bst1107I 103 GTATAC GTA^TAC
BstEII 1621 GGTGACC G^GT[ACGT]ACC
BstPI 1621 GGTGACC G^GT[ACGT]ACC
BstZ17I 103 GTATAC GTA^TAC
ClaI 1963 ATCGAT AT^CGAT
EagI 4071 CGGCCG C^GGCCG
Eam1105I 2820 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4071 CGGCCG C^GGCCG
Eco52I 4071 CGGCCG C^GGCCG
EcoO65I 1621 GGTGACC G^GT[ACGT]ACC
EcoT22I 2038 ATGCAT ATGCA^T
HindIII 3791 AAGCTT A^AGCTT
HpaI 1198 GTTAAC GTT^AAC
KasI 4195 GGCGCC G^GCGCC
KspI 4031 CCGCGG CCGC^GG
MfeI 40 CAATTG C^AATTG
MunI 40 CAATTG C^AATTG
NaeI 3617 GCCGGC GCC^GGC
NarI 4195 GGCGCC GG^CGCC
NdeI 2120 CATATG CA^TATG
NgoMIV 3617 GCCGGC G^CCGGC
NsiI 2038 ATGCAT ATGCA^T
PflFI 586 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 7 CACGTG CAC^GTG
PmlI 7 CACGTG CAC^GTG
PshAI 2399 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1439 TCATGA T^CATGA
SacI 3434 GAGCTC GAGCT^C
SacII 4031 CCGCGG CCGC^GG
SapI 1167 GAAGAGC GCTCTTC(1/4)
SexAI 1776 ACCAGGT A^CC[AT]GGT
SfiI 2747 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4195 GGCGCC GGC^GCC
SspBI 1557 TGTACA T^GTACA
Tth111I 586 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2942 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 991 GACGTC G[GA]^CG[CT]C
4195 GGCGCC
Alw44I 145 GTGCAC G^TGCAC
2125 GTGCAC
ApaLI 145 GTGCAC G^TGCAC
2125 GTGCAC
BalI 1102 TGGCCA TGG^CCA
2366 TGGCCA
BbvCI 460 GCTGAGG CCTCAGC(-5/-2)
2504 GCTGAGG
BciVI 3400 GGATAC GTATCC(6/5)
2162 GTATCC
BsaAI 7 CACGTG [CT]AC^GT[GA]
996 CACGTA
BsaHI 991 GACGTC G[GA]^CG[CT]C
4195 GGCGCC
BsaI 4006 GAGACC GGTCTC(1/5)
3534 GGTCTC
BsmI 1211 GCATTC GAATGC(1/-1)
3353 GCATTC
BsrDI 668 GCAATG GCAATG(2/0)
3058 GCAATG
BsrFI 3013 ACCGGC [GA]^CCGG[CT]
3617 GCCGGC
BssSI 385 CTCGTG CACGAG(-5/-1)
4047 CTCGTG
BstAPI 72 GCATGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2339 GCACGGGCTGC
BstXI 776 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCACATCCCTGG
Cfr10I 3013 ACCGGC [GA]^CCGG[CT]
3617 GCCGGC
DraI 1228 TTTAAA TTT^AAA
3630 TTTAAA
DraIII 2544 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3225 CACAGAGTG
HgaI 3780 GCGTC GACGC(5/10)
4156 GCGTC
Hin1I 991 GACGTC G[GA]^CG[CT]C
4195 GGCGCC
HincII 634 GTCAAC GT[CT]^[GA]AC
1198 GTTAAC
HindII 634 GTCAAC GT[CT]^[GA]AC
1198 GTTAAC
MluNI 1102 TGGCCA TGG^CCA
2366 TGGCCA
MscI 1102 TGGCCA TGG^CCA
2366 TGGCCA
PflMI 2291 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4010 CCAGCTGGTGG
SmlI 1996 CTCAAG C^T[CT][GA]AG
3045 CTTGAG
Van91I 2291 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4010 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AccIII AciI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BspLU11I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI