##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspHI
AvrII BamHI BbrPI BbsI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SspI
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 4089 ACACGT A^C[GA][CT]GT
AhdI 3795 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1727 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3795 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3361 TGCGCA TGC^GCA
BbeI 2197 GGCGCC GGCGC^C
BsmI 2828 GCATTC GAATGC(1/-1)
BspHI 1178 TCATGA T^CATGA
BsrBI 3643 GAGCGG CCGCTC(-3/-3)
BsrDI 3184 GCAATG GCAATG(2/0)
BstEII 3901 GGTGACC G^GT[ACGT]ACC
BstPI 3901 GGTGACC G^GT[ACGT]ACC
DraI 159 TTTAAA TTT^AAA
DraIII 2283 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3432 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3795 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1784 CTCTTC CTCTTC(1/4)
EcoNI 1651 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3901 GGTGACC G^GT[ACGT]ACC
EcoRI 3453 GAATTC G^AATTC
FspI 3361 TGCGCA TGC^GCA
HindIII 1927 AAGCTT A^AGCTT
KasI 2197 GGCGCC G^GCGCC
Ksp632I 1784 CTCTTC CTCTTC(1/4)
NarI 2197 GGCGCC GG^CGCC
NcoI 2992 CCATGG C^CATGG
NspI 469 GCATGC [GA]CATG^[CT]
PaeR7I 13 CTCGAG C^TCGAG
PshAI 3724 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1178 TCATGA T^CATGA
SapI 1783 GCTCTTC GCTCTTC(1/4)
SbfI 2670 CCTGCAGG CCTGCA^GG
SexAI 3690 ACCAGGT A^CC[AT]GGT
SfoI 2197 GGCGCC GGC^GCC
SphI 469 GCATGC GCATG^C
Sse8387I 2670 CCTGCAGG CCTGCA^GG
SwaI 158 ATTTAAAT ATTT^AAAT
XhoI 13 CTCGAG C^TCGAG
XmnI 1727 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 24 AAATTC [GA]^AATT[CT]
3453 GAATTC
AfaI 2375 GTAC GT^AC
3335 GTAC
ApoI 24 AAATTC [GA]^AATT[CT]
3453 GAATTC
BcgI 908 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2157 CGATTCTCCTGC
BlpI 1961 GCTCAGC GC^T[ACGT]AGC
3552 GCTCAGC
Bpu1102I 1961 GCTCAGC GC^T[ACGT]AGC
3552 GCTCAGC
Bsp1407I 2374 TGTACA T^GTACA
3334 TGTACA
BsrFI 2491 GCCGGT [GA]^CCGG[CT]
3701 GCCGGT
BsrGI 2374 TGTACA T^GTACA
3334 TGTACA
CelII 1961 GCTCAGC GC^T[ACGT]AGC
3552 GCTCAGC
Cfr10I 2491 GCCGGT [GA]^CCGG[CT]
3701 GCCGGT
HaeII 2197 GGCGCC [GA]GCGC^[CT]
3224 GGCGCT
KspI 3023 CCGCGG CCGC^GG
3326 CCGCGG
RsaI 2375 GTAC GT^AC
3335 GTAC
SacII 3023 CCGCGG CCGC^GG
3326 CCGCGG
SspBI 2374 TGTACA T^GTACA
3334 TGTACA
StuI 1729 AGGCCT AGG^CCT
1853 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AocI ApaI AspI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI