##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AviII BbeI BbvCI
BclI BfrI BglII BsaBI BsaI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraIII Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
KasI KpnI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AseI 3977 ATTAAT AT^TAAT
AsnI 3977 ATTAAT AT^TAAT
AspI 2018 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3921 CCTAGG C^CTAGG
BamHI 3065 GGATCC G^GATCC
BbrPI 2446 CACGTG CAC^GTG
BcgI 1708 CGAGCTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3087 GTATCC GTATCC(6/5)
BlnI 3921 CCTAGG C^CTAGG
BsaAI 2446 CACGTG [CT]AC^GT[GA]
BsaWI 1791 ACCGGA [AT]^CCGG[AT]
BsiEI 2226 CGGCCG CG[GA][CT]^CG
BsmI 1179 GCATTC GAATGC(1/-1)
BspLU11I 2690 ACATGT A^CATGT
BssSI 3485 CTCGTG CACGAG(-5/-1)
BstEII 1187 GGTGACC G^GT[ACGT]ACC
BstPI 1187 GGTGACC G^GT[ACGT]ACC
BstYI 3065 GGATCC [GA]^GATC[CT]
CfoI 3527 GCGC GCG^C
DraI 4247 TTTAAA TTT^AAA
EagI 2226 CGGCCG C^GGCCG
EclXI 2226 CGGCCG C^GGCCG
Eco52I 2226 CGGCCG C^GGCCG
EcoO65I 1187 GGTGACC G^GT[ACGT]ACC
HhaI 3527 GCGC GCG^C
HinP1I 3527 GCGC G^CGC
HincII 4404 GTTAAC GT[CT]^[GA]AC
HindII 4404 GTTAAC GT[CT]^[GA]AC
HindIII 4292 AAGCTT A^AGCTT
HpaI 4404 GTTAAC GTT^AAC
MflI 3065 GGATCC [GA]^GATC[CT]
NaeI 1933 GCCGGC GCC^GGC
NgoMIV 1933 GCCGGC G^CCGGC
PflFI 2018 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2446 CACGTG CAC^GTG
PmlI 2446 CACGTG CAC^GTG
PshBI 3977 ATTAAT AT^TAAT
SapI 2816 GCTCTTC GCTCTTC(1/4)
SpeI 4261 ACTAGT A^CTAGT
Tth111I 2018 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2938 TCTAGA T^CTAGA
XhoII 3065 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 836 GTAGAC GT^[AC][GT]AC
1862 GTCTAC
AcsI 1784 AAATTT [GA]^AATT[CT]
4065 AAATTT
AflIII 1902 ACGTGT A^C[GA][CT]GT
2690 ACATGT
Alw44I 1883 GTGCAC G^TGCAC
3131 GTGCAC
AlwI 3066 GATCC GGATC(4/5)
3065 GGATC
ApaLI 1883 GTGCAC G^TGCAC
3131 GTGCAC
ApoI 1784 AAATTT [GA]^AATT[CT]
4065 AAATTT
BalI 1807 TGGCCA TGG^CCA
3497 TGGCCA
BbsI 3151 GAAGAC GAAGAC(2/6)
31 GTCTTC
BpuAI 3151 GAAGAC GAAGAC(2/6)
31 GTCTTC
Bsp1407I 1917 TGTACA T^GTACA
3206 TGTACA
BsrGI 1917 TGTACA T^GTACA
3206 TGTACA
BstXI 1978 CCAGGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2623 CCATCGAGGTGG
DrdI 22 GACACCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1404 GACTCCAGGGTC
KspI 77 CCGCGG CCGC^GG
2121 CCGCGG
MluNI 1807 TGGCCA TGG^CCA
3497 TGGCCA
MscI 1807 TGGCCA TGG^CCA
3497 TGGCCA
NdeI 2563 CATATG CA^TATG
3187 CATATG
SacI 2425 GAGCTC GAGCT^C
3377 GAGCTC
SacII 77 CCGCGG CCGC^GG
2121 CCGCGG
SphI 1773 GCATGC GCATG^C
2269 GCATGC
SspBI 1917 TGTACA T^GTACA
3206 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CelII Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfiI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI