##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbeI BcgI BciVI BclI BfrI
BlnI BsaBI BsaHI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BssHII BstBI BstUI ClaI CpoI
DrdI EagI EclXI Eco52I EcoRI
EcoRV FbaI FseI FspI Hin1I
HpaI KasI KspI MamI MluI
MroI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2738 GGTACC G^GTACC
AccI 2544 GTATAC GT^[AC][GT]AC
AclI 1630 AACGTT AA^CGTT
AflIII 948 ACACGT A^C[GA][CT]GT
Aor51HI 695 AGCGCT AGC^GCT
Asp718I 2738 GGTACC G^GTACC
BbrPI 949 CACGTG CAC^GTG
BsaAI 949 CACGTG [CT]AC^GT[GA]
BsiEI 1009 CGATCG CG[GA][CT]^CG
BsmBI 588 CGTCTC CGTCTC(1/5)
Bst1107I 2544 GTATAC GTA^TAC
BstAPI 155 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 290 GGTGACC G^GT[ACGT]ACC
BstPI 290 GGTGACC G^GT[ACGT]ACC
BstZ17I 2544 GTATAC GTA^TAC
DraI 4102 TTTAAA TTT^AAA
Eco47III 695 AGCGCT AGC^GCT
EcoO65I 290 GGTGACC G^GT[ACGT]ACC
HaeII 695 AGCGCT [GA]GCGC^[CT]
HindIII 514 AAGCTT A^AGCTT
KpnI 2738 GGTACC GGTAC^C
NaeI 3227 GCCGGC GCC^GGC
NcoI 251 CCATGG C^CATGG
NdeI 3578 CATATG CA^TATG
NgoMIV 3227 GCCGGC G^CCGGC
PmaCI 949 CACGTG CAC^GTG
PmlI 949 CACGTG CAC^GTG
Psp1406I 1630 AACGTT AA^CGTT
PvuI 1009 CGATCG CGAT^CG
SspI 4152 AATATT AAT^ATT
XbaI 4047 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 1819 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3688 GACTAGCTGTC
AspEI 1819 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3688 GACTAGCTGTC
BbsI 2773 GAAGAC GAAGAC(2/6)
3935 GAAGAC
BglII 3856 AGATCT A^GATCT
4233 AGATCT
BlpI 34 GCTGAGC GC^T[ACGT]AGC
2308 GCTGAGC
Bpu1102I 34 GCTGAGC GC^T[ACGT]AGC
2308 GCTGAGC
BpuAI 2773 GAAGAC GAAGAC(2/6)
3935 GAAGAC
Bsp1407I 2676 TGTACA T^GTACA
4228 TGTACA
BspHI 764 TCATGA T^CATGA
3981 TCATGA
BsrDI 1382 CATTGC GCAATG(2/0)
3175 GCAATG
BsrGI 2676 TGTACA T^GTACA
4228 TGTACA
BssSI 71 CACGAG CACGAG(-5/-1)
1487 CACGAG
CelII 34 GCTGAGC GC^T[ACGT]AGC
2308 GCTGAGC
Eam1105I 1819 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3688 GACTAGCTGTC
EcoT22I 811 ATGCAT ATGCA^T
3581 ATGCAT
MfeI 195 CAATTG C^AATTG
682 CAATTG
MunI 195 CAATTG C^AATTG
682 CAATTG
NsiI 811 ATGCAT ATGCA^T
3581 ATGCAT
RcaI 764 TCATGA T^CATGA
3981 TCATGA
SapI 2269 GAAGAGC GCTCTTC(1/4)
4131 GAAGAGC
SmaI 1025 CCCGGG CCC^GGG
3332 CCCGGG
SspBI 2676 TGTACA T^GTACA
4228 TGTACA
TaqI 1139 TCGA T^CGA
3276 TCGA
TthHB8I 1139 TCGA T^CGA
3276 TCGA
XcmI 232 CCAGCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2604 CCAGGTAGAAGCTGG
XmaCI 1025 CCCGGG C^CCGGG
3332 CCCGGG
XmaI 1025 CCCGGG C^CCGGG
3332 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII