##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
BbeI BsaHI BsiEI BsiWI BsmI
BspDI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI Hin1I HpaI KasI KpnI
KspI MluI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 3606 GTCGAC GT^[AC][GT]AC
AccII 195 CGCG CG^CG
AccIII 598 TCCGGA T^CCGGA
AflII 3106 CTTAAG C^TTAAG
Asp700I 12 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1091 GAGCTC G[AT]GC[AT]^C
AspI 1876 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2582 CCCGAG C^[CT]CG[GA]G
AviII 121 TGCGCA TGC^GCA
AvrII 2087 CCTAGG C^CTAGG
BamHI 3209 GGATCC G^GATCC
BbsI 288 GAAGAC GAAGAC(2/6)
BcgI 298 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3821 GGATAC GTATCC(6/5)
BclI 3665 TGATCA T^GATCA
BcnI 1362 CCCGG CC^[GC]GG
BfrI 3106 CTTAAG C^TTAAG
BlnI 2087 CCTAGG C^CTAGG
BlpI 3053 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3053 GCTGAGC GC^T[ACGT]AGC
BpuAI 288 GAAGAC GAAGAC(2/6)
BsaBI 2105 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 598 TCCGGA [AT]^CCGG[AT]
BseAI 598 TCCGGA T^CCGGA
BsiHKAI 1091 GAGCTC G[AT]GC[AT]^C
BsmBI 798 GAGACG CGTCTC(1/5)
BsoBI 2582 CCCGAG C^[CT]CG[GA]G
BspEI 598 TCCGGA T^CCGGA
BsrFI 148 GCCGGC [GA]^CCGG[CT]
BstAPI 2176 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 195 CGCG CG^CG
CelII 3053 GCTGAGC GC^T[ACGT]AGC
Cfr10I 148 GCCGGC [GA]^CCGG[CT]
EcoRV 2252 GATATC GAT^ATC
FbaI 3665 TGATCA T^GATCA
FspI 121 TGCGCA TGC^GCA
HaeII 487 AGCGCC [GA]GCGC^[CT]
HgaI 511 GCGTC GACGC(5/10)
HindIII 815 AAGCTT A^AGCTT
MamI 2105 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3659 CAATTG C^AATTG
MroI 598 TCCGGA T^CCGGA
MunI 3659 CAATTG C^AATTG
MvnI 195 CGCG CG^CG
NaeI 148 GCCGGC GCC^GGC
NciI 1362 CCCGG CC^[GC]GG
NgoMIV 148 GCCGGC G^CCGGC
PflFI 1876 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4314 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1091 GAGCTC GAGCT^C
SalI 3606 GTCGAC G^TCGAC
SapI 563 GCTCTTC GCTCTTC(1/4)
SphI 1610 GCATGC GCATG^C
StuI 310 AGGCCT AGG^CCT
Tth111I 1876 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 12 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1066 ACGTGT A^C[GA][CT]GT
3398 ACGTGT
BalI 1549 TGGCCA TGG^CCA
3029 TGGCCA
BanII 1091 GAGCTC G[GA]GC[CT]^C
3056 GAGCCC
BbvCI 212 CCTCAGC CCTCAGC(-5/-2)
1639 GCTGAGG
BsaI 770 GAGACC GGTCTC(1/5)
2828 GGTCTC
BseRI 3433 CTCCTC GAGGAG(10/8)
795 GAGGAG
Bsp1407I 2134 TGTACA T^GTACA
2851 TGTACA
BspHI 805 TCATGA T^CATGA
2143 TCATGA
BsrBI 2207 CCGCTC CCGCTC(-3/-3)
2609 CCGCTC
BsrDI 1889 GCAATG GCAATG(2/0)
4411 GCAATG
BsrGI 2134 TGTACA T^GTACA
2851 TGTACA
DraI 2876 TTTAAA TTT^AAA
3254 TTTAAA
EaeI 1549 TGGCCA [CT]^GGCC[GA]
3029 TGGCCA
EcoT22I 1251 ATGCAT ATGCA^T
2442 ATGCAT
MluNI 1549 TGGCCA TGG^CCA
3029 TGGCCA
MscI 1549 TGGCCA TGG^CCA
3029 TGGCCA
NsiI 1251 ATGCAT ATGCA^T
2442 ATGCAT
PflMI 89 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1624 CCAGCTACTGG
RcaI 805 TCATGA T^CATGA
2143 TCATGA
SspBI 2134 TGTACA T^GTACA
2851 TGTACA
TaqI 1537 TCGA T^CGA
3607 TCGA
TthHB8I 1537 TCGA T^CGA
3607 TCGA
Van91I 89 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1624 CCAGCTACTGG
XbaI 2166 TCTAGA T^CTAGA
4011 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaII BanI
BbrPI BbvI BfaI BglI BglII
BmyI BpmI BsaAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII