##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI Asp718I AspI AvrII
BbeI BcgI BglI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspMI
BsrGI BssHII BstBI BstEII BstPI
CelII ClaI CpoI DraI DrdI
Eco47III EcoO65I EcoRV HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI Psp1406I PvuI PvuII RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SspBI SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 6 GGCGTC G[GA]^CG[CT]C
AflII 2011 CTTAAG C^TTAAG
AhdI 620 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 406 GGGCCC GGGCC^C
AseI 3319 ATTAAT AT^TAAT
AsnI 3319 ATTAAT AT^TAAT
AspEI 620 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3487 TGCGCA TGC^GCA
BbrPI 2120 CACGTG CAC^GTG
BbvCI 4312 GCTGAGG CCTCAGC(-5/-2)
BciVI 2185 GGATAC GTATCC(6/5)
BfrI 2011 CTTAAG C^TTAAG
BsaBI 2475 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6 GGCGTC G[GA]^CG[CT]C
BsiEI 43 CGGCCG CG[GA][CT]^CG
BspLU11I 308 ACATGT A^CATGT
BsrFI 45 GCCGGC [GA]^CCGG[CT]
Bst1107I 2682 GTATAC GTA^TAC
BstAPI 4344 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2682 GTATAC GTA^TAC
Cfr10I 45 GCCGGC [GA]^CCGG[CT]
DraIII 1758 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 43 CGGCCG C^GGCCG
Eam1105I 620 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
FseI 44 GGCCGGCC GGCCGG^CC
FspI 3487 TGCGCA TGC^GCA
HgaI 7 GCGTC GACGC(5/10)
Hin1I 6 GGCGTC G[GA]^CG[CT]C
MamI 2475 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 860 CAGCGG C[AC]G^C[GT]G
NaeI 45 GCCGGC GCC^GGC
NcoI 3047 CCATGG C^CATGG
NgoMIV 45 GCCGGC G^CCGGC
PmaCI 2120 CACGTG CAC^GTG
PmlI 2120 CACGTG CAC^GTG
PshAI 3586 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3319 ATTAAT AT^TAAT
SapI 781 GCTCTTC GCTCTTC(1/4)
SbfI 2088 CCTGCAGG CCTGCA^GG
ScaI 2808 AGTACT AGT^ACT
SmaI 101 CCCGGG CCC^GGG
SpeI 3003 ACTAGT A^CTAGT
SphI 2947 GCATGC GCATG^C
Sse8387I 2088 CCTGCAGG CCTGCA^GG
XmaCI 101 CCCGGG C^CCGGG
XmaI 101 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2514 GTCTAC GT^[AC][GT]AC
2682 GTATAC
AccII 1069 CGCG CG^CG
1869 CGCG
AocI 1156 CCTGAGG CC^T[ACGT]AGG
4194 CCTGAGG
BalI 2307 TGGCCA TGG^CCA
4222 TGGCCA
BbsI 301 GAAGAC GAAGAC(2/6)
595 GAAGAC
BclI 2411 TGATCA T^GATCA
3756 TGATCA
BpuAI 301 GAAGAC GAAGAC(2/6)
595 GAAGAC
BsaAI 2120 CACGTG [CT]AC^GT[GA]
2859 TACGTG
BsmBI 749 CGTCTC CGTCTC(1/5)
4242 CGTCTC
BspHI 813 TCATGA T^CATGA
1497 TCATGA
BsrBI 96 CCGCTC CCGCTC(-3/-3)
4184 GAGCGG
BsrDI 874 GCAATG GCAATG(2/0)
4065 GCAATG
BstUI 1069 CGCG CG^CG
1869 CGCG
Bsu36I 1156 CCTGAGG CC^T[ACGT]AGG
4194 CCTGAGG
Eco81I 1156 CCTGAGG CC^T[ACGT]AGG
4194 CCTGAGG
EcoNI 522 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3595 CCTACCCAAGG
EcoT22I 1859 ATGCAT ATGCA^T
2652 ATGCAT
FbaI 2411 TGATCA T^GATCA
3756 TGATCA
HaeII 84 AGCGCC [GA]GCGC^[CT]
380 GGCGCT
HincII 1029 GTCAAC GT[CT]^[GA]AC
3935 GTCAAC
HindII 1029 GTCAAC GT[CT]^[GA]AC
3935 GTCAAC
HindIII 1269 AAGCTT A^AGCTT
1688 AAGCTT
MluNI 2307 TGGCCA TGG^CCA
4222 TGGCCA
MscI 2307 TGGCCA TGG^CCA
4222 TGGCCA
MvnI 1069 CGCG CG^CG
1869 CGCG
NsiI 1859 ATGCAT ATGCA^T
2652 ATGCAT
PaeR7I 699 CTCGAG C^TCGAG
1736 CTCGAG
PflMI 834 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGGCAATGG
PstI 547 CTGCAG CTGCA^G
2089 CTGCAG
RcaI 813 TCATGA T^CATGA
1497 TCATGA
Van91I 834 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGGCAATGG
XbaI 591 TCTAGA T^CTAGA
4115 TCTAGA
XhoI 699 CTCGAG C^TCGAG
1736 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI