##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AspEI AspI AviII AvrII BamHI
BclI BfrI BlnI BseAI BsiWI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshAI Psp1406I PvuI RsrII SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2914 GGTACC G^GTACC
Alw44I 1303 GTGCAC G^TGCAC
AocI 89 CCTCAGG CC^T[ACGT]AGG
ApaI 3953 GGGCCC GGGCC^C
ApaLI 1303 GTGCAC G^TGCAC
AseI 1220 ATTAAT AT^TAAT
AsnI 1220 ATTAAT AT^TAAT
Asp700I 2752 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2914 GGTACC G^GTACC
BalI 293 TGGCCA TGG^CCA
BbrPI 3915 CACGTG CAC^GTG
BcgI 3005 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1219 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 223 GAGACC GGTCTC(1/5)
BsmBI 3353 CGTCTC CGTCTC(1/5)
BsmI 4009 GAATGC GAATGC(1/-1)
Bsp1407I 4149 TGTACA T^GTACA
BspHI 1036 TCATGA T^CATGA
BspLU11I 4224 ACATGT A^CATGT
BsrGI 4149 TGTACA T^GTACA
BstAPI 99 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 89 CCTCAGG CC^T[ACGT]AGG
Eco81I 89 CCTCAGG CC^T[ACGT]AGG
HincII 3867 GTCGAC GT[CT]^[GA]AC
HindII 3867 GTCGAC GT[CT]^[GA]AC
KpnI 2914 GGTACC GGTAC^C
KspI 50 CCGCGG CCGC^GG
MamI 1219 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1986 ACGCGT A^CGCGT
MluNI 293 TGGCCA TGG^CCA
MscI 293 TGGCCA TGG^CCA
NaeI 61 GCCGGC GCC^GGC
NgoMIV 61 GCCGGC G^CCGGC
PmaCI 3915 CACGTG CAC^GTG
PmlI 3915 CACGTG CAC^GTG
PpuMI 3334 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1220 ATTAAT AT^TAAT
PvuII 460 CAGCTG CAG^CTG
RcaI 1036 TCATGA T^CATGA
SacI 250 GAGCTC GAGCT^C
SacII 50 CCGCGG CCGC^GG
SalI 3867 GTCGAC G^TCGAC
SexAI 644 ACCTGGT A^CC[AT]GGT
SspBI 4149 TGTACA T^GTACA
StuI 1942 AGGCCT AGG^CCT
XcmI 3221 CCAAGAGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2752 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 430 GACGTC GACGT^C
2629 GACGTC
AccI 2243 GTAGAC GT^[AC][GT]AC
3867 GTCGAC
AgeI 3907 ACCGGT A^CCGGT
3927 ACCGGT
BbsI 2546 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BbvCI 3016 CCTCAGC CCTCAGC(-5/-2)
3114 CCTCAGC
BciVI 1126 GGATAC GTATCC(6/5)
269 GTATCC
BglI 2135 GCCACACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3279 GCCAAAGGGGC
BglII 1688 AGATCT A^GATCT
2777 AGATCT
BlpI 2151 GCTCAGC GC^T[ACGT]AGC
3966 GCTCAGC
Bpu1102I 2151 GCTCAGC GC^T[ACGT]AGC
3966 GCTCAGC
BpuAI 2546 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BsaWI 3907 ACCGGT [AT]^CCGG[AT]
3927 ACCGGT
BsrDI 2570 CATTGC GCAATG(2/0)
2956 GCAATG
CelII 2151 GCTCAGC GC^T[ACGT]AGC
3966 GCTCAGC
PaeR7I 3330 CTCGAG C^TCGAG
3648 CTCGAG
PflMI 639 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3092 CCAGATTCTGG
PinAI 3907 ACCGGT A^CCGGT
3927 ACCGGT
PstI 32 CTGCAG CTGCA^G
2046 CTGCAG
SspI 1139 AATATT AAT^ATT
2432 AATATT
Van91I 639 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3092 CCAGATTCTGG
XhoI 3330 CTCGAG C^TCGAG
3648 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmaCI XmaI