##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AviII AvrII BbsI BcgI BclI
BfrI BlnI BpuAI BseAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI EcoT22I
FbaI FspI HindIII KpnI KspI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 1276 CGCG CG^CG
AhdI 3059 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 644 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3059 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2287 GGATCC G^GATCC
BbeI 2592 GGCGCC GGCGC^C
BbvCI 3754 GCTGAGG CCTCAGC(-5/-2)
BglI 4446 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 25 CGGCCG CG[GA][CT]^CG
BsrBI 537 GAGCGG CCGCTC(-3/-3)
BsrDI 614 GCAATG GCAATG(2/0)
BstAPI 2354 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 521 GGTAACC G^GT[ACGT]ACC
BstPI 521 GGTAACC G^GT[ACGT]ACC
BstUI 1276 CGCG CG^CG
DraI 577 TTTAAA TTT^AAA
DsaI 1590 CCATGG C^C[GA][CT]GG
EagI 25 CGGCCG C^GGCCG
Eam1105I 3059 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 25 CGGCCG C^GGCCG
Eco52I 25 CGGCCG C^GGCCG
EcoO65I 521 GGTAACC G^GT[ACGT]ACC
EcoRV 3568 GATATC GAT^ATC
FseI 22 GGCCGGCC GGCCGG^CC
HaeII 2592 GGCGCC [GA]GCGC^[CT]
HincII 3517 GTTAAC GT[CT]^[GA]AC
HindII 3517 GTTAAC GT[CT]^[GA]AC
HpaI 3517 GTTAAC GTT^AAC
KasI 2592 GGCGCC G^GCGCC
MvnI 1276 CGCG CG^CG
NarI 2592 GGCGCC GG^CGCC
NcoI 1590 CCATGG C^CATGG
NdeI 4325 CATATG CA^TATG
PaeR7I 986 CTCGAG C^TCGAG
PshAI 1193 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 2592 GGCGCC GGC^GCC
SmaI 3466 CCCGGG CCC^GGG
SpeI 4085 ACTAGT A^CTAGT
SspI 4188 AATATT AAT^ATT
XhoI 986 CTCGAG C^TCGAG
XmaCI 3466 CCCGGG C^CCGGG
XmaI 3466 CCCGGG C^CCGGG
XmnI 644 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 2030 GAATTT [GA]^AATT[CT]
4098 GAATTT
ApoI 2030 GAATTT [GA]^AATT[CT]
4098 GAATTT
AspI 1525 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3146 GACAGGGTC
BciVI 700 GGATAC GTATCC(6/5)
813 GTATCC
BglII 1132 AGATCT A^GATCT
1561 AGATCT
BsaBI 1138 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1160 GATATTGATC
BsaWI 2922 ACCGGA [AT]^CCGG[AT]
3810 TCCGGT
BsgI 1332 CTGCAC GTGCAG(16/14)
2006 GTGCAG
BsmI 1420 GAATGC GAATGC(1/-1)
4156 GAATGC
Bsp1407I 1623 TGTACA T^GTACA
3072 TGTACA
BsrFI 23 GCCGGC [GA]^CCGG[CT]
27 GCCGGC
BsrGI 1623 TGTACA T^GTACA
3072 TGTACA
Cfr10I 23 GCCGGC [GA]^CCGG[CT]
27 GCCGGC
HgaI 89 GACGC GACGC(5/10)
1712 GCGTC
MamI 1138 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1160 GATATTGATC
NaeI 23 GCCGGC GCC^GGC
27 GCCGGC
NgoMIV 23 GCCGGC G^CCGGC
27 GCCGGC
NspI 3698 GCATGT [GA]CATG^[CT]
3785 GCATGT
PflFI 1525 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3146 GACAGGGTC
PflMI 1100 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4022 CCAACAACTGG
SspBI 1623 TGTACA T^GTACA
3072 TGTACA
Tth111I 1525 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3146 GACAGGGTC
Van91I 1100 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4022 CCAACAACTGG
XbaI 4152 TCTAGA T^CTAGA
4306 TCTAGA
XcmI 2649 CCATCCTGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2770 CCAGGAGGTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DrdI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII